Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4507 and GFF4508 are separated by 126 nucleotides GFF4508 and GFF4509 are separated by 0 nucleotides GFF4509 and GFF4510 overlap by 28 nucleotides
GFF4507 - Glycerol dehydrogenase (EC 1.1.1.6), at 293,890 to 294,978
GFF4507
GFF4508 - Aspartate aminotransferase (EC 2.6.1.1), at 295,105 to 296,265
GFF4508
GFF4509 - Co-activator of prophage gene expression IbrB, at 296,266 to 296,883
GFF4509
GFF4510 - Co-activator of prophage gene expression IbrA, at 296,856 to 297,965
GFF4510
Position (kb) 295
296
297 Strain fitness (log2 ratio) -1
0
1 at 294.230 kb on + strand, within GFF4507 at 294.460 kb on - strand, within GFF4507 at 294.482 kb on + strand, within GFF4507 at 294.482 kb on + strand, within GFF4507 at 294.483 kb on - strand, within GFF4507 at 294.582 kb on - strand, within GFF4507 at 294.582 kb on - strand, within GFF4507 at 294.707 kb on - strand, within GFF4507 at 294.825 kb on - strand, within GFF4507 at 295.352 kb on + strand, within GFF4508 at 295.353 kb on - strand, within GFF4508 at 295.417 kb on + strand, within GFF4508 at 295.486 kb on + strand, within GFF4508 at 295.529 kb on + strand, within GFF4508 at 295.624 kb on - strand, within GFF4508 at 295.725 kb on + strand, within GFF4508 at 295.725 kb on + strand, within GFF4508 at 295.725 kb on + strand, within GFF4508 at 295.726 kb on - strand, within GFF4508 at 295.821 kb on + strand, within GFF4508 at 295.894 kb on + strand, within GFF4508 at 295.895 kb on - strand, within GFF4508 at 296.131 kb on + strand, within GFF4508 at 296.197 kb on - strand at 296.199 kb on - strand at 296.199 kb on - strand at 296.199 kb on - strand at 296.263 kb on + strand at 296.271 kb on + strand at 296.361 kb on + strand, within GFF4509 at 296.398 kb on + strand, within GFF4509 at 296.665 kb on - strand, within GFF4509 at 296.715 kb on + strand, within GFF4509 at 296.724 kb on + strand, within GFF4509 at 296.788 kb on + strand, within GFF4509 at 296.788 kb on + strand, within GFF4509 at 296.789 kb on - strand, within GFF4509 at 296.883 kb on + strand at 296.916 kb on - strand at 296.916 kb on - strand at 297.116 kb on + strand, within GFF4510 at 297.146 kb on + strand, within GFF4510 at 297.147 kb on - strand, within GFF4510 at 297.153 kb on + strand, within GFF4510 at 297.242 kb on - strand, within GFF4510 at 297.242 kb on - strand, within GFF4510
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 294,230 + GFF4507 0.31 -0.1 294,460 - GFF4507 0.52 +0.0 294,482 + GFF4507 0.54 +0.1 294,482 + GFF4507 0.54 +0.2 294,483 - GFF4507 0.54 -1.4 294,582 - GFF4507 0.64 -0.2 294,582 - GFF4507 0.64 -0.9 294,707 - GFF4507 0.75 +0.3 294,825 - GFF4507 0.86 +0.6 295,352 + GFF4508 0.21 -0.1 295,353 - GFF4508 0.21 +1.0 295,417 + GFF4508 0.27 +0.2 295,486 + GFF4508 0.33 +0.1 295,529 + GFF4508 0.37 +0.1 295,624 - GFF4508 0.45 +0.5 295,725 + GFF4508 0.53 -0.0 295,725 + GFF4508 0.53 +1.2 295,725 + GFF4508 0.53 +0.2 295,726 - GFF4508 0.53 +0.1 295,821 + GFF4508 0.62 -0.2 295,894 + GFF4508 0.68 -0.2 295,895 - GFF4508 0.68 +0.2 296,131 + GFF4508 0.88 +0.3 296,197 - +0.2 296,199 - +0.1 296,199 - -0.3 296,199 - -0.8 296,263 + +0.1 296,271 + -0.1 296,361 + GFF4509 0.15 -0.4 296,398 + GFF4509 0.21 -0.6 296,665 - GFF4509 0.65 -0.1 296,715 + GFF4509 0.73 +0.2 296,724 + GFF4509 0.74 -0.1 296,788 + GFF4509 0.84 +0.2 296,788 + GFF4509 0.84 -0.5 296,789 - GFF4509 0.85 -0.0 296,883 + +0.0 296,916 - +0.0 296,916 - -0.1 297,116 + GFF4510 0.23 -0.1 297,146 + GFF4510 0.26 -0.3 297,147 - GFF4510 0.26 -0.1 297,153 + GFF4510 0.27 -0.3 297,242 - GFF4510 0.35 -0.8 297,242 - GFF4510 0.35 -0.1
Or see this region's nucleotide sequence