Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4412 and GFF4413 overlap by 30 nucleotides GFF4413 and GFF4414 overlap by 4 nucleotides GFF4414 and GFF4415 are separated by 185 nucleotides
GFF4412 - Arylesterase precursor (EC 3.1.1.2), at 196,068 to 196,682
GFF4412
GFF4413 - Uncharacterized metabolite ABC transporter in Enterobacteriaceae, ATP-binding protein EC-YbbA, at 196,653 to 197,339
GFF4413
GFF4414 - FIG00809136: hypothetical protein, at 197,336 to 199,750
GFF4414
GFF4415 - Putative outer membrane protein, at 199,936 to 201,069
GFF4415
Position (kb)
197
198
199
200 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 196.345 kb on - strand, within GFF4412 at 196.380 kb on + strand, within GFF4412 at 196.472 kb on + strand, within GFF4412 at 196.482 kb on + strand, within GFF4412 at 196.526 kb on + strand, within GFF4412 at 196.527 kb on - strand, within GFF4412 at 196.593 kb on + strand, within GFF4412 at 196.950 kb on - strand, within GFF4413 at 196.950 kb on - strand, within GFF4413 at 197.324 kb on - strand at 197.340 kb on + strand at 197.341 kb on - strand at 197.341 kb on - strand at 197.485 kb on + strand at 197.552 kb on - strand at 197.640 kb on + strand, within GFF4414 at 197.641 kb on - strand, within GFF4414 at 197.645 kb on - strand, within GFF4414 at 197.645 kb on - strand, within GFF4414 at 197.867 kb on - strand, within GFF4414 at 198.205 kb on + strand, within GFF4414 at 198.208 kb on + strand, within GFF4414 at 198.538 kb on + strand, within GFF4414 at 198.539 kb on - strand, within GFF4414 at 198.539 kb on - strand, within GFF4414 at 198.613 kb on + strand, within GFF4414 at 198.728 kb on - strand, within GFF4414 at 198.809 kb on + strand, within GFF4414 at 198.823 kb on + strand, within GFF4414 at 199.107 kb on + strand, within GFF4414 at 199.135 kb on + strand, within GFF4414 at 199.419 kb on + strand, within GFF4414 at 199.434 kb on + strand, within GFF4414 at 199.434 kb on + strand, within GFF4414 at 199.485 kb on + strand, within GFF4414 at 199.486 kb on - strand, within GFF4414 at 199.728 kb on + strand at 199.916 kb on + strand at 200.137 kb on - strand, within GFF4415 at 200.219 kb on - strand, within GFF4415 at 200.221 kb on + strand, within GFF4415 at 200.222 kb on - strand, within GFF4415 at 200.289 kb on + strand, within GFF4415 at 200.309 kb on - strand, within GFF4415 at 200.309 kb on - strand, within GFF4415 at 200.369 kb on - strand, within GFF4415 at 200.518 kb on + strand, within GFF4415 at 200.545 kb on + strand, within GFF4415 at 200.648 kb on - strand, within GFF4415 at 200.648 kb on - strand, within GFF4415 at 200.698 kb on - strand, within GFF4415
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 196,345 - GFF4412 0.45 +0.3 196,380 + GFF4412 0.51 -3.0 196,472 + GFF4412 0.66 +0.1 196,482 + GFF4412 0.67 -0.0 196,526 + GFF4412 0.74 +1.2 196,527 - GFF4412 0.75 +1.3 196,593 + GFF4412 0.85 +0.7 196,950 - GFF4413 0.43 +0.6 196,950 - GFF4413 0.43 +0.9 197,324 - +0.1 197,340 + +1.0 197,341 - +0.7 197,341 - +0.2 197,485 + -0.0 197,552 - +0.1 197,640 + GFF4414 0.13 +0.1 197,641 - GFF4414 0.13 +0.9 197,645 - GFF4414 0.13 +0.1 197,645 - GFF4414 0.13 -0.6 197,867 - GFF4414 0.22 -1.3 198,205 + GFF4414 0.36 -0.5 198,208 + GFF4414 0.36 +0.3 198,538 + GFF4414 0.50 +0.8 198,539 - GFF4414 0.50 -0.1 198,539 - GFF4414 0.50 -0.0 198,613 + GFF4414 0.53 +0.4 198,728 - GFF4414 0.58 -0.0 198,809 + GFF4414 0.61 -0.1 198,823 + GFF4414 0.62 -0.5 199,107 + GFF4414 0.73 -0.2 199,135 + GFF4414 0.74 -1.4 199,419 + GFF4414 0.86 +0.3 199,434 + GFF4414 0.87 +0.4 199,434 + GFF4414 0.87 -0.5 199,485 + GFF4414 0.89 -0.4 199,486 - GFF4414 0.89 -0.7 199,728 + -0.7 199,916 + -0.8 200,137 - GFF4415 0.18 +0.2 200,219 - GFF4415 0.25 +0.3 200,221 + GFF4415 0.25 -0.1 200,222 - GFF4415 0.25 +0.1 200,289 + GFF4415 0.31 +0.1 200,309 - GFF4415 0.33 -0.5 200,309 - GFF4415 0.33 -0.2 200,369 - GFF4415 0.38 +1.1 200,518 + GFF4415 0.51 +0.0 200,545 + GFF4415 0.54 -0.6 200,648 - GFF4415 0.63 -0.6 200,648 - GFF4415 0.63 -0.2 200,698 - GFF4415 0.67 +0.5
Or see this region's nucleotide sequence