Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4136 and GFF4137 are separated by 10 nucleotides GFF4137 and GFF4138 overlap by 4 nucleotides GFF4138 and GFF4139 overlap by 4 nucleotides GFF4139 and GFF4140 are separated by 112 nucleotides
GFF4136 - Oxidoreductase, Gfo/Idh/MocA family, at 19,075 to 20,088
GFF4136
GFF4137 - PTS system, glucitol/sorbitol-specific IIB component and second of two IIC components (EC 2.7.1.69), at 20,099 to 21,079
GFF4137
GFF4138 - PTS system, glucitol/sorbitol-specific IIA component (EC 2.7.1.69), at 21,076 to 21,450
GFF4138
GFF4139 - PTS system, glucitol/sorbitol-specific IIC component (EC 2.7.1.69), at 21,447 to 21,968
GFF4139
GFF4140 - putative cytoplasmic protein, at 22,081 to 22,365
GFF4140
Position (kb) 21
22 Strain fitness (log2 ratio) -1
0
1 at 20.106 kb on - strand at 20.662 kb on + strand, within GFF4137 at 20.663 kb on - strand, within GFF4137 at 20.742 kb on - strand, within GFF4137 at 20.760 kb on + strand, within GFF4137 at 20.760 kb on + strand, within GFF4137 at 20.760 kb on + strand, within GFF4137 at 20.761 kb on - strand, within GFF4137 at 20.761 kb on - strand, within GFF4137 at 20.875 kb on + strand, within GFF4137 at 21.038 kb on - strand at 21.064 kb on + strand at 21.065 kb on - strand at 21.255 kb on - strand, within GFF4138 at 21.429 kb on + strand at 21.488 kb on - strand at 21.519 kb on + strand, within GFF4139 at 21.520 kb on - strand, within GFF4139 at 21.703 kb on - strand, within GFF4139 at 22.083 kb on - strand at 22.158 kb on - strand, within GFF4140
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 20,106 - +0.2 20,662 + GFF4137 0.57 +0.6 20,663 - GFF4137 0.57 +0.2 20,742 - GFF4137 0.66 +0.0 20,760 + GFF4137 0.67 -0.4 20,760 + GFF4137 0.67 +0.2 20,760 + GFF4137 0.67 -0.1 20,761 - GFF4137 0.67 +0.4 20,761 - GFF4137 0.67 +1.2 20,875 + GFF4137 0.79 +0.1 21,038 - +0.4 21,064 + -0.0 21,065 - -0.4 21,255 - GFF4138 0.48 +0.5 21,429 + +0.3 21,488 - +0.0 21,519 + GFF4139 0.14 -0.6 21,520 - GFF4139 0.14 -0.0 21,703 - GFF4139 0.49 +0.8 22,083 - -1.3 22,158 - GFF4140 0.27 -0.4
Or see this region's nucleotide sequence