Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4021 and GFF4022 are separated by 297 nucleotides GFF4022 and GFF4023 overlap by 4 nucleotides GFF4023 and GFF4024 are separated by 185 nucleotides GFF4024 and GFF4025 are separated by 39 nucleotides
GFF4021 - FIG01200701: possible membrane protein, at 288,965 to 289,318
GFF4021
GFF4022 - UPF0033 protein YedF, at 289,616 to 289,849
GFF4022
GFF4023 - Putative transport system permease protein, at 289,846 to 291,051
GFF4023
GFF4024 - FIG00640199: hypothetical protein, at 291,237 to 291,662
GFF4024
GFF4025 - Cytoplasmic alpha-amylase (EC 3.2.1.1), at 291,702 to 293,186
GFF4025
Position (kb) 289
290
291
292 Strain fitness (log2 ratio) -1
0
1
2 at 288.891 kb on + strand at 289.181 kb on - strand, within GFF4021 at 289.930 kb on + strand at 289.941 kb on - strand at 289.986 kb on + strand, within GFF4023 at 290.085 kb on - strand, within GFF4023 at 290.200 kb on - strand, within GFF4023 at 290.228 kb on + strand, within GFF4023 at 290.270 kb on + strand, within GFF4023 at 290.326 kb on - strand, within GFF4023 at 290.614 kb on + strand, within GFF4023 at 290.750 kb on - strand, within GFF4023 at 290.855 kb on + strand, within GFF4023 at 290.856 kb on - strand, within GFF4023 at 290.960 kb on + strand at 291.123 kb on - strand at 291.123 kb on - strand at 291.129 kb on - strand at 291.496 kb on + strand, within GFF4024 at 291.525 kb on + strand, within GFF4024 at 291.830 kb on - strand at 291.935 kb on + strand, within GFF4025 at 291.960 kb on + strand, within GFF4025 at 291.961 kb on - strand, within GFF4025
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 288,891 + +0.2 289,181 - GFF4021 0.61 +0.2 289,930 + -0.0 289,941 - -1.0 289,986 + GFF4023 0.12 +0.7 290,085 - GFF4023 0.20 +0.3 290,200 - GFF4023 0.29 -0.1 290,228 + GFF4023 0.32 -0.2 290,270 + GFF4023 0.35 +0.7 290,326 - GFF4023 0.40 +0.6 290,614 + GFF4023 0.64 +0.4 290,750 - GFF4023 0.75 +0.1 290,855 + GFF4023 0.84 +0.3 290,856 - GFF4023 0.84 +0.0 290,960 + +0.5 291,123 - +2.3 291,123 - -0.3 291,129 - -0.2 291,496 + GFF4024 0.61 +0.0 291,525 + GFF4024 0.68 -0.1 291,830 - -0.3 291,935 + GFF4025 0.16 -0.3 291,960 + GFF4025 0.17 +1.1 291,961 - GFF4025 0.17 -0.2
Or see this region's nucleotide sequence