Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3719 and GFF3720 are separated by 265 nucleotides GFF3720 and GFF3721 overlap by 1 nucleotides GFF3721 and GFF3722 are separated by 10 nucleotides GFF3722 and GFF3723 are separated by 94 nucleotides
GFF3719 - Glutathione-regulated potassium-efflux system ATP-binding protein, at 23,202 to 25,109
GFF3719
GFF3720 - Glutathione-regulated potassium-efflux system ancillary protein KefG, at 25,375 to 25,926
GFF3720
GFF3721 - Glutathione-regulated potassium-efflux system protein KefB, at 25,926 to 27,731
GFF3721
GFF3722 - Putative cytoplasmic protein ,probably associated with Glutathione-regulated potassium-efflux, at 27,742 to 27,942
GFF3722
GFF3723 - FKBP-type peptidyl-prolyl cis-trans isomerase SlyD (EC 5.2.1.8), at 28,037 to 28,627
GFF3723
Position (kb) 25
26
27
28 Strain fitness (log2 ratio) -2
-1
0
1 at 24.992 kb on + strand at 25.037 kb on + strand at 25.119 kb on + strand at 25.120 kb on - strand at 25.156 kb on + strand at 25.194 kb on - strand at 25.253 kb on + strand at 25.254 kb on - strand at 25.254 kb on - strand at 25.282 kb on + strand at 25.283 kb on - strand at 25.283 kb on - strand at 25.283 kb on - strand at 25.319 kb on + strand at 25.320 kb on - strand at 25.345 kb on - strand at 25.345 kb on - strand at 25.405 kb on + strand at 25.406 kb on - strand at 25.406 kb on - strand at 25.478 kb on + strand, within GFF3720 at 25.548 kb on - strand, within GFF3720 at 25.586 kb on - strand, within GFF3720 at 25.591 kb on + strand, within GFF3720 at 25.591 kb on + strand, within GFF3720 at 25.591 kb on + strand, within GFF3720 at 25.591 kb on + strand, within GFF3720 at 25.591 kb on + strand, within GFF3720 at 25.591 kb on + strand, within GFF3720 at 25.591 kb on + strand, within GFF3720 at 25.591 kb on + strand, within GFF3720 at 25.592 kb on - strand, within GFF3720 at 25.592 kb on - strand, within GFF3720 at 25.592 kb on - strand, within GFF3720 at 25.592 kb on - strand, within GFF3720 at 25.592 kb on - strand, within GFF3720 at 25.592 kb on - strand, within GFF3720 at 25.592 kb on - strand, within GFF3720 at 25.592 kb on - strand, within GFF3720 at 25.593 kb on + strand, within GFF3720 at 25.593 kb on + strand, within GFF3720 at 25.594 kb on - strand, within GFF3720 at 25.685 kb on - strand, within GFF3720 at 25.732 kb on + strand, within GFF3720 at 25.824 kb on + strand, within GFF3720 at 25.824 kb on + strand, within GFF3720 at 26.023 kb on + strand at 26.133 kb on - strand, within GFF3721 at 26.536 kb on + strand, within GFF3721 at 26.750 kb on + strand, within GFF3721 at 26.763 kb on - strand, within GFF3721 at 26.763 kb on - strand, within GFF3721 at 26.783 kb on - strand, within GFF3721 at 26.788 kb on - strand, within GFF3721 at 26.798 kb on - strand, within GFF3721 at 26.798 kb on - strand, within GFF3721 at 26.798 kb on - strand, within GFF3721 at 26.823 kb on - strand, within GFF3721 at 26.871 kb on + strand, within GFF3721 at 26.871 kb on + strand, within GFF3721 at 26.872 kb on - strand, within GFF3721 at 26.879 kb on - strand, within GFF3721 at 26.995 kb on + strand, within GFF3721 at 26.996 kb on - strand, within GFF3721 at 26.998 kb on + strand, within GFF3721 at 27.056 kb on + strand, within GFF3721 at 27.056 kb on + strand, within GFF3721 at 27.057 kb on - strand, within GFF3721 at 27.122 kb on + strand, within GFF3721 at 27.122 kb on + strand, within GFF3721 at 27.123 kb on - strand, within GFF3721 at 27.179 kb on - strand, within GFF3721 at 27.255 kb on + strand, within GFF3721 at 27.256 kb on - strand, within GFF3721 at 27.257 kb on + strand, within GFF3721 at 27.257 kb on + strand, within GFF3721 at 27.258 kb on - strand, within GFF3721 at 27.264 kb on + strand, within GFF3721 at 27.278 kb on + strand, within GFF3721 at 27.405 kb on - strand, within GFF3721 at 27.445 kb on + strand, within GFF3721 at 27.445 kb on + strand, within GFF3721 at 27.445 kb on + strand, within GFF3721 at 27.446 kb on - strand, within GFF3721 at 27.509 kb on + strand, within GFF3721 at 27.527 kb on - strand, within GFF3721 at 27.527 kb on - strand, within GFF3721 at 27.530 kb on + strand, within GFF3721 at 27.530 kb on + strand, within GFF3721 at 27.531 kb on - strand, within GFF3721 at 27.582 kb on - strand at 27.615 kb on - strand at 27.625 kb on + strand at 27.850 kb on - strand, within GFF3722 at 27.969 kb on - strand at 28.034 kb on - strand at 28.044 kb on + strand at 28.080 kb on + strand at 28.080 kb on + strand at 28.277 kb on - strand, within GFF3723 at 28.437 kb on + strand, within GFF3723 at 28.662 kb on + strand at 28.686 kb on - strand at 28.686 kb on - strand at 28.686 kb on - strand at 28.686 kb on - strand at 28.686 kb on - strand at 28.686 kb on - strand at 28.686 kb on - strand at 28.689 kb on + strand at 28.689 kb on + strand at 28.690 kb on - strand at 28.690 kb on - strand at 28.693 kb on + strand at 28.693 kb on + strand at 28.694 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 24,992 + -0.3 25,037 + +1.0 25,119 + -0.1 25,120 - +0.1 25,156 + +0.2 25,194 - -0.8 25,253 + +0.9 25,254 - +1.5 25,254 - -0.1 25,282 + +0.9 25,283 - +0.1 25,283 - -0.4 25,283 - +0.2 25,319 + -0.2 25,320 - -0.4 25,345 - -0.3 25,345 - -0.5 25,405 + -0.9 25,406 - +1.6 25,406 - -0.1 25,478 + GFF3720 0.19 +0.1 25,548 - GFF3720 0.31 +0.3 25,586 - GFF3720 0.38 +1.3 25,591 + GFF3720 0.39 -0.1 25,591 + GFF3720 0.39 -0.0 25,591 + GFF3720 0.39 -0.1 25,591 + GFF3720 0.39 -0.1 25,591 + GFF3720 0.39 -0.2 25,591 + GFF3720 0.39 -1.0 25,591 + GFF3720 0.39 +0.3 25,591 + GFF3720 0.39 +0.9 25,592 - GFF3720 0.39 +0.5 25,592 - GFF3720 0.39 +0.3 25,592 - GFF3720 0.39 +0.2 25,592 - GFF3720 0.39 +0.1 25,592 - GFF3720 0.39 -0.2 25,592 - GFF3720 0.39 -0.3 25,592 - GFF3720 0.39 -0.1 25,592 - GFF3720 0.39 -0.1 25,593 + GFF3720 0.39 -0.5 25,593 + GFF3720 0.39 +0.2 25,594 - GFF3720 0.40 -0.4 25,685 - GFF3720 0.56 -0.3 25,732 + GFF3720 0.65 +0.1 25,824 + GFF3720 0.81 -0.1 25,824 + GFF3720 0.81 -0.2 26,023 + +0.2 26,133 - GFF3721 0.11 -0.4 26,536 + GFF3721 0.34 +0.8 26,750 + GFF3721 0.46 +0.2 26,763 - GFF3721 0.46 +0.1 26,763 - GFF3721 0.46 +0.8 26,783 - GFF3721 0.47 +0.6 26,788 - GFF3721 0.48 +0.5 26,798 - GFF3721 0.48 +0.1 26,798 - GFF3721 0.48 +0.2 26,798 - GFF3721 0.48 -0.1 26,823 - GFF3721 0.50 +0.4 26,871 + GFF3721 0.52 -0.2 26,871 + GFF3721 0.52 +0.9 26,872 - GFF3721 0.52 +0.9 26,879 - GFF3721 0.53 +0.4 26,995 + GFF3721 0.59 +0.1 26,996 - GFF3721 0.59 -0.1 26,998 + GFF3721 0.59 -0.4 27,056 + GFF3721 0.63 -0.0 27,056 + GFF3721 0.63 -0.2 27,057 - GFF3721 0.63 -0.6 27,122 + GFF3721 0.66 +0.5 27,122 + GFF3721 0.66 +0.2 27,123 - GFF3721 0.66 -0.0 27,179 - GFF3721 0.69 +1.2 27,255 + GFF3721 0.74 -0.1 27,256 - GFF3721 0.74 +0.4 27,257 + GFF3721 0.74 -0.5 27,257 + GFF3721 0.74 -0.2 27,258 - GFF3721 0.74 +0.2 27,264 + GFF3721 0.74 +0.5 27,278 + GFF3721 0.75 -0.2 27,405 - GFF3721 0.82 +0.3 27,445 + GFF3721 0.84 -1.3 27,445 + GFF3721 0.84 -0.4 27,445 + GFF3721 0.84 +0.3 27,446 - GFF3721 0.84 +0.0 27,509 + GFF3721 0.88 +0.6 27,527 - GFF3721 0.89 +0.3 27,527 - GFF3721 0.89 -0.0 27,530 + GFF3721 0.89 +0.0 27,530 + GFF3721 0.89 -0.1 27,531 - GFF3721 0.89 -0.6 27,582 - +0.0 27,615 - +0.6 27,625 + -0.8 27,850 - GFF3722 0.54 -0.5 27,969 - -1.8 28,034 - -0.2 28,044 + +0.2 28,080 + +0.1 28,080 + -0.6 28,277 - GFF3723 0.41 -0.7 28,437 + GFF3723 0.68 -0.3 28,662 + -0.5 28,686 - -1.3 28,686 - +0.0 28,686 - -0.6 28,686 - -0.3 28,686 - +0.5 28,686 - -0.4 28,686 - +0.1 28,689 + -0.3 28,689 + -1.0 28,690 - -0.2 28,690 - +0.3 28,693 + +0.3 28,693 + +0.1 28,694 - +0.5
Or see this region's nucleotide sequence