Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3590 and GFF3591 are separated by 99 nucleotides GFF3591 and GFF3592 are separated by 2 nucleotides GFF3592 and GFF3593 are separated by 57 nucleotides GFF3593 and GFF3594 are separated by 15 nucleotides
GFF3590 - Secretion system effector SseC, at 347,050 to 348,504
GFF3590
GFF3591 - Secretion system effector SseD, at 348,604 to 349,107
GFF3591
GFF3592 - Secretion system effector SseE, at 349,110 to 349,526
GFF3592
GFF3593 - Secretion system chaparone SscB, at 349,584 to 350,012
GFF3593
GFF3594 - Type III secretion effector SseF, at 350,028 to 350,810
GFF3594
Position (kb) 348
349
350 Strain fitness (log2 ratio) -2
-1
0
1 at 347.623 kb on + strand, within GFF3590 at 347.624 kb on - strand, within GFF3590 at 347.624 kb on - strand, within GFF3590 at 347.624 kb on - strand, within GFF3590 at 347.712 kb on + strand, within GFF3590 at 347.727 kb on + strand, within GFF3590 at 347.868 kb on + strand, within GFF3590 at 347.868 kb on + strand, within GFF3590 at 348.436 kb on - strand at 348.443 kb on + strand at 348.444 kb on - strand at 348.446 kb on + strand at 348.447 kb on - strand at 348.836 kb on + strand, within GFF3591 at 348.948 kb on + strand, within GFF3591 at 349.168 kb on - strand, within GFF3592 at 349.186 kb on + strand, within GFF3592 at 349.190 kb on - strand, within GFF3592 at 349.230 kb on + strand, within GFF3592 at 349.316 kb on - strand, within GFF3592 at 349.336 kb on + strand, within GFF3592 at 349.578 kb on - strand at 349.578 kb on - strand at 350.107 kb on + strand, within GFF3594
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 347,623 + GFF3590 0.39 -0.5 347,624 - GFF3590 0.39 +1.3 347,624 - GFF3590 0.39 -0.6 347,624 - GFF3590 0.39 -0.6 347,712 + GFF3590 0.45 -1.4 347,727 + GFF3590 0.47 +0.5 347,868 + GFF3590 0.56 +0.2 347,868 + GFF3590 0.56 -0.1 348,436 - -0.0 348,443 + -1.9 348,444 - -0.3 348,446 + -0.1 348,447 - +0.2 348,836 + GFF3591 0.46 +0.0 348,948 + GFF3591 0.68 +0.9 349,168 - GFF3592 0.14 +0.4 349,186 + GFF3592 0.18 -0.0 349,190 - GFF3592 0.19 -0.6 349,230 + GFF3592 0.29 +0.4 349,316 - GFF3592 0.49 +0.3 349,336 + GFF3592 0.54 +0.1 349,578 - -0.1 349,578 - +1.0 350,107 + GFF3594 0.10 +1.1
Or see this region's nucleotide sequence