Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3377 and GFF3378 are separated by 123 nucleotides GFF3378 and GFF3379 are separated by 2 nucleotides GFF3379 and GFF3380 overlap by 11 nucleotides GFF3380 and GFF3381 overlap by 4 nucleotides
GFF3377 - 3-oxoacyl-[acyl-carrier-protein] synthase, KASII (EC 2.3.1.41), at 142,523 to 143,728
GFF3377
GFF3378 - Aminodeoxychorismate lyase (EC 4.1.3.38), at 143,852 to 144,661
GFF3378
GFF3379 - FIG004453: protein YceG like, at 144,664 to 145,686
GFF3379
GFF3380 - Thymidylate kinase (EC 2.7.4.9), at 145,676 to 146,317
GFF3380
GFF3381 - DNA polymerase III delta prime subunit (EC 2.7.7.7), at 146,314 to 147,318
GFF3381
Position (kb) 144
145
146 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 143.698 kb on + strand at 143.726 kb on + strand at 143.790 kb on - strand at 143.892 kb on + strand at 144.507 kb on + strand, within GFF3378 at 144.575 kb on - strand, within GFF3378 at 144.765 kb on - strand at 144.769 kb on - strand, within GFF3379 at 144.783 kb on + strand, within GFF3379 at 144.836 kb on - strand, within GFF3379 at 145.043 kb on + strand, within GFF3379 at 145.044 kb on - strand, within GFF3379 at 145.068 kb on + strand, within GFF3379 at 145.069 kb on - strand, within GFF3379 at 145.166 kb on - strand, within GFF3379 at 145.166 kb on - strand, within GFF3379 at 145.360 kb on - strand, within GFF3379 at 145.429 kb on + strand, within GFF3379 at 145.430 kb on - strand, within GFF3379 at 145.430 kb on - strand, within GFF3379 at 145.430 kb on - strand, within GFF3379 at 145.431 kb on + strand, within GFF3379 at 145.484 kb on - strand, within GFF3379 at 145.485 kb on + strand, within GFF3379
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 143,698 + -1.6 143,726 + -3.5 143,790 - +0.2 143,892 + +0.3 144,507 + GFF3378 0.81 -0.3 144,575 - GFF3378 0.89 -0.6 144,765 - -1.7 144,769 - GFF3379 0.10 +0.4 144,783 + GFF3379 0.12 +1.3 144,836 - GFF3379 0.17 -2.3 145,043 + GFF3379 0.37 +1.2 145,044 - GFF3379 0.37 +0.2 145,068 + GFF3379 0.39 +0.0 145,069 - GFF3379 0.40 -0.6 145,166 - GFF3379 0.49 +0.6 145,166 - GFF3379 0.49 +0.2 145,360 - GFF3379 0.68 -1.5 145,429 + GFF3379 0.75 +0.4 145,430 - GFF3379 0.75 +0.2 145,430 - GFF3379 0.75 +0.0 145,430 - GFF3379 0.75 +0.1 145,431 + GFF3379 0.75 +0.2 145,484 - GFF3379 0.80 +0.8 145,485 + GFF3379 0.80 -0.3
Or see this region's nucleotide sequence