Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF332 and GFF333 are separated by 7 nucleotides GFF333 and GFF334 are separated by 98 nucleotides GFF334 and GFF335 are separated by 266 nucleotides
GFF332 - Mg(2+) transport ATPase protein C, at 47,913 to 48,590
GFF332
GFF333 - hypothetical protein, at 48,598 to 48,711
GFF333
GFF334 - Mg(2+) transport ATPase, P-type (EC 3.6.3.2), at 48,810 to 51,536
GFF334
GFF335 - Putative inner membrane protein, at 51,803 to 52,330
GFF335
Position (kb)
48
49
50
51
52 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 47.869 kb on - strand at 47.993 kb on + strand, within GFF332 at 47.993 kb on + strand, within GFF332 at 47.994 kb on - strand, within GFF332 at 48.302 kb on - strand, within GFF332 at 48.341 kb on + strand, within GFF332 at 48.436 kb on + strand, within GFF332 at 48.437 kb on - strand, within GFF332 at 48.626 kb on + strand, within GFF333 at 48.964 kb on + strand at 49.075 kb on - strand at 49.199 kb on - strand, within GFF334 at 49.226 kb on + strand, within GFF334 at 49.437 kb on - strand, within GFF334 at 49.472 kb on + strand, within GFF334 at 49.490 kb on + strand, within GFF334 at 49.567 kb on + strand, within GFF334 at 49.713 kb on - strand, within GFF334 at 49.960 kb on + strand, within GFF334 at 49.974 kb on - strand, within GFF334 at 50.008 kb on + strand, within GFF334 at 50.048 kb on + strand, within GFF334 at 50.048 kb on + strand, within GFF334 at 50.049 kb on - strand, within GFF334 at 50.170 kb on + strand, within GFF334 at 50.261 kb on - strand, within GFF334 at 50.261 kb on - strand, within GFF334 at 50.344 kb on - strand, within GFF334 at 50.365 kb on + strand, within GFF334 at 50.384 kb on + strand, within GFF334 at 50.384 kb on + strand, within GFF334 at 50.384 kb on + strand, within GFF334 at 50.384 kb on + strand, within GFF334 at 50.385 kb on - strand, within GFF334 at 50.416 kb on + strand, within GFF334 at 50.442 kb on - strand, within GFF334 at 50.847 kb on - strand, within GFF334 at 50.850 kb on - strand, within GFF334 at 50.924 kb on + strand, within GFF334 at 50.925 kb on - strand, within GFF334 at 51.045 kb on + strand, within GFF334 at 51.141 kb on - strand, within GFF334 at 51.294 kb on - strand at 51.320 kb on + strand at 51.349 kb on + strand at 51.384 kb on + strand at 51.384 kb on + strand at 51.386 kb on + strand at 51.475 kb on - strand at 51.535 kb on - strand at 51.592 kb on + strand at 51.625 kb on + strand at 51.626 kb on - strand at 51.778 kb on - strand at 51.896 kb on - strand, within GFF335 at 52.000 kb on + strand, within GFF335 at 52.073 kb on - strand, within GFF335 at 52.073 kb on - strand, within GFF335 at 52.076 kb on + strand, within GFF335 at 52.076 kb on + strand, within GFF335 at 52.076 kb on + strand, within GFF335 at 52.077 kb on - strand, within GFF335 at 52.077 kb on - strand, within GFF335 at 52.122 kb on - strand, within GFF335 at 52.131 kb on + strand, within GFF335 at 52.131 kb on + strand, within GFF335 at 52.211 kb on - strand, within GFF335 at 52.211 kb on - strand, within GFF335 at 52.225 kb on - strand, within GFF335 at 52.242 kb on - strand, within GFF335 at 52.265 kb on - strand, within GFF335 at 52.329 kb on - strand at 52.526 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 47,869 - -3.2 47,993 + GFF332 0.12 -0.9 47,993 + GFF332 0.12 -1.2 47,994 - GFF332 0.12 -3.4 48,302 - GFF332 0.57 -2.9 48,341 + GFF332 0.63 -1.7 48,436 + GFF332 0.77 -0.9 48,437 - GFF332 0.77 -2.4 48,626 + GFF333 0.25 -0.4 48,964 + +1.1 49,075 - -0.3 49,199 - GFF334 0.14 -0.7 49,226 + GFF334 0.15 -0.6 49,437 - GFF334 0.23 +0.1 49,472 + GFF334 0.24 -0.5 49,490 + GFF334 0.25 -0.5 49,567 + GFF334 0.28 -0.0 49,713 - GFF334 0.33 -0.4 49,960 + GFF334 0.42 -0.3 49,974 - GFF334 0.43 -1.5 50,008 + GFF334 0.44 -0.4 50,048 + GFF334 0.45 -0.7 50,048 + GFF334 0.45 -0.3 50,049 - GFF334 0.45 -0.5 50,170 + GFF334 0.50 -0.5 50,261 - GFF334 0.53 -0.9 50,261 - GFF334 0.53 -0.2 50,344 - GFF334 0.56 -0.0 50,365 + GFF334 0.57 -0.1 50,384 + GFF334 0.58 -1.3 50,384 + GFF334 0.58 -2.2 50,384 + GFF334 0.58 +0.7 50,384 + GFF334 0.58 -0.0 50,385 - GFF334 0.58 -0.0 50,416 + GFF334 0.59 -0.2 50,442 - GFF334 0.60 -0.9 50,847 - GFF334 0.75 +0.2 50,850 - GFF334 0.75 -0.2 50,924 + GFF334 0.78 -0.9 50,925 - GFF334 0.78 -0.2 51,045 + GFF334 0.82 -0.9 51,141 - GFF334 0.85 -0.0 51,294 - -0.4 51,320 + -0.6 51,349 + -1.0 51,384 + -1.3 51,384 + -0.0 51,386 + -1.2 51,475 - -0.2 51,535 - +0.4 51,592 + +0.1 51,625 + +0.3 51,626 - -0.3 51,778 - +0.8 51,896 - GFF335 0.18 -0.4 52,000 + GFF335 0.37 +0.7 52,073 - GFF335 0.51 -0.2 52,073 - GFF335 0.51 +0.5 52,076 + GFF335 0.52 -0.1 52,076 + GFF335 0.52 +0.0 52,076 + GFF335 0.52 +0.2 52,077 - GFF335 0.52 -0.1 52,077 - GFF335 0.52 +0.7 52,122 - GFF335 0.60 -0.4 52,131 + GFF335 0.62 +0.3 52,131 + GFF335 0.62 -1.5 52,211 - GFF335 0.77 -0.1 52,211 - GFF335 0.77 +0.7 52,225 - GFF335 0.80 +0.3 52,242 - GFF335 0.83 -0.3 52,265 - GFF335 0.88 +0.6 52,329 - +0.4 52,526 - -0.3
Or see this region's nucleotide sequence