Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3195 and GFF3196 are separated by 2 nucleotides GFF3196 and GFF3197 overlap by 4 nucleotides GFF3197 and GFF3198 are separated by 144 nucleotides GFF3198 and GFF3199 are separated by 67 nucleotides
GFF3195 - Uncharacterized membrane protein YqjD, at 201,933 to 202,238
GFF3195
GFF3196 - Inner membrane protein YqjE, at 202,241 to 202,639
GFF3196
GFF3197 - Inner membrane protein YqjK, at 202,636 to 202,935
GFF3197
GFF3198 - Inner membrane protein YqjF, at 203,080 to 203,565
GFF3198
GFF3199 - Glutathione S-transferase, omega (EC 2.5.1.18), at 203,633 to 204,619
GFF3199
Position (kb) 203
204 Strain fitness (log2 ratio) -1
0
1 at 202.155 kb on + strand, within GFF3195 at 202.220 kb on - strand at 202.350 kb on + strand, within GFF3196 at 202.767 kb on + strand, within GFF3197 at 202.775 kb on + strand, within GFF3197 at 202.812 kb on - strand, within GFF3197 at 202.842 kb on + strand, within GFF3197 at 202.842 kb on + strand, within GFF3197 at 202.842 kb on + strand, within GFF3197 at 202.843 kb on - strand, within GFF3197 at 202.843 kb on - strand, within GFF3197 at 202.843 kb on - strand, within GFF3197 at 202.843 kb on - strand, within GFF3197 at 203.281 kb on + strand, within GFF3198 at 203.281 kb on + strand, within GFF3198 at 203.282 kb on - strand, within GFF3198 at 203.327 kb on + strand, within GFF3198 at 203.406 kb on + strand, within GFF3198 at 203.453 kb on + strand, within GFF3198 at 203.454 kb on - strand, within GFF3198 at 203.504 kb on + strand, within GFF3198 at 203.573 kb on - strand at 203.574 kb on + strand at 203.583 kb on - strand at 203.735 kb on - strand, within GFF3199 at 203.990 kb on + strand, within GFF3199 at 204.062 kb on - strand, within GFF3199 at 204.062 kb on - strand, within GFF3199 at 204.093 kb on + strand, within GFF3199 at 204.095 kb on - strand, within GFF3199 at 204.140 kb on + strand, within GFF3199 at 204.141 kb on - strand, within GFF3199 at 204.262 kb on - strand, within GFF3199 at 204.270 kb on + strand, within GFF3199 at 204.270 kb on + strand, within GFF3199 at 204.318 kb on - strand, within GFF3199 at 204.460 kb on + strand, within GFF3199 at 204.461 kb on - strand, within GFF3199 at 204.471 kb on + strand, within GFF3199 at 204.506 kb on - strand, within GFF3199
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 202,155 + GFF3195 0.73 -0.1 202,220 - +0.6 202,350 + GFF3196 0.27 -0.5 202,767 + GFF3197 0.44 -0.8 202,775 + GFF3197 0.46 -0.1 202,812 - GFF3197 0.59 -0.4 202,842 + GFF3197 0.69 +0.4 202,842 + GFF3197 0.69 +0.3 202,842 + GFF3197 0.69 +0.2 202,843 - GFF3197 0.69 +0.4 202,843 - GFF3197 0.69 +0.9 202,843 - GFF3197 0.69 -0.1 202,843 - GFF3197 0.69 -0.4 203,281 + GFF3198 0.41 +0.3 203,281 + GFF3198 0.41 +0.7 203,282 - GFF3198 0.42 +0.2 203,327 + GFF3198 0.51 +0.9 203,406 + GFF3198 0.67 +0.5 203,453 + GFF3198 0.77 -0.0 203,454 - GFF3198 0.77 -0.5 203,504 + GFF3198 0.87 +0.7 203,573 - +0.1 203,574 + +0.1 203,583 - -0.2 203,735 - GFF3199 0.10 +0.3 203,990 + GFF3199 0.36 +0.0 204,062 - GFF3199 0.43 -0.9 204,062 - GFF3199 0.43 -0.1 204,093 + GFF3199 0.47 +0.3 204,095 - GFF3199 0.47 +0.6 204,140 + GFF3199 0.51 -0.3 204,141 - GFF3199 0.51 +0.3 204,262 - GFF3199 0.64 +0.2 204,270 + GFF3199 0.65 +0.2 204,270 + GFF3199 0.65 -0.0 204,318 - GFF3199 0.69 +1.7 204,460 + GFF3199 0.84 +0.4 204,461 - GFF3199 0.84 +1.2 204,471 + GFF3199 0.85 +0.2 204,506 - GFF3199 0.88 +0.9
Or see this region's nucleotide sequence