Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3179 and GFF3180 are separated by 234 nucleotides GFF3180 and GFF3181 overlap by 4 nucleotides
GFF3179 - iron-chelator utilization protein, at 184,294 to 185,061
GFF3179
GFF3180 - Transcriptional regulator, PadR family, at 185,296 to 185,940
GFF3180
GFF3181 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 185,937 to 187,502
GFF3181
Position (kb)
185
186 Strain fitness (log2 ratio)
-1
0
1
2 at 184.392 kb on + strand, within GFF3179 at 184.476 kb on - strand, within GFF3179 at 184.476 kb on - strand, within GFF3179 at 184.485 kb on + strand, within GFF3179 at 184.485 kb on + strand, within GFF3179 at 184.486 kb on - strand, within GFF3179 at 184.598 kb on - strand, within GFF3179 at 184.629 kb on - strand, within GFF3179 at 184.833 kb on + strand, within GFF3179 at 184.975 kb on - strand, within GFF3179 at 184.977 kb on + strand, within GFF3179 at 184.978 kb on - strand, within GFF3179 at 184.978 kb on - strand, within GFF3179 at 184.978 kb on - strand, within GFF3179 at 185.044 kb on + strand at 185.337 kb on + strand at 185.552 kb on - strand, within GFF3180 at 185.652 kb on + strand, within GFF3180 at 185.823 kb on + strand, within GFF3180 at 185.997 kb on + strand at 186.108 kb on - strand, within GFF3181 at 186.108 kb on - strand, within GFF3181 at 186.150 kb on + strand, within GFF3181 at 186.189 kb on - strand, within GFF3181 at 186.549 kb on - strand, within GFF3181 at 186.762 kb on + strand, within GFF3181 at 186.763 kb on - strand, within GFF3181 at 186.794 kb on + strand, within GFF3181 at 186.876 kb on - strand, within GFF3181 at 186.877 kb on + strand, within GFF3181 at 186.877 kb on + strand, within GFF3181 at 186.878 kb on - strand, within GFF3181 at 186.878 kb on - strand, within GFF3181 at 186.879 kb on - strand, within GFF3181 at 186.927 kb on + strand, within GFF3181 at 186.927 kb on + strand, within GFF3181
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 184,392 + GFF3179 0.13 -0.2 184,476 - GFF3179 0.24 +1.6 184,476 - GFF3179 0.24 -0.0 184,485 + GFF3179 0.25 +0.3 184,485 + GFF3179 0.25 -1.7 184,486 - GFF3179 0.25 -0.7 184,598 - GFF3179 0.40 -1.4 184,629 - GFF3179 0.44 -0.4 184,833 + GFF3179 0.70 +0.0 184,975 - GFF3179 0.89 +0.7 184,977 + GFF3179 0.89 +0.4 184,978 - GFF3179 0.89 +0.6 184,978 - GFF3179 0.89 +0.1 184,978 - GFF3179 0.89 +1.0 185,044 + +0.2 185,337 + +0.5 185,552 - GFF3180 0.40 -0.8 185,652 + GFF3180 0.55 -0.0 185,823 + GFF3180 0.82 -0.7 185,997 + +2.1 186,108 - GFF3181 0.11 -0.3 186,108 - GFF3181 0.11 +0.4 186,150 + GFF3181 0.14 +0.1 186,189 - GFF3181 0.16 -0.6 186,549 - GFF3181 0.39 +0.1 186,762 + GFF3181 0.53 +0.2 186,763 - GFF3181 0.53 +0.4 186,794 + GFF3181 0.55 -0.5 186,876 - GFF3181 0.60 +0.4 186,877 + GFF3181 0.60 -0.7 186,877 + GFF3181 0.60 +0.7 186,878 - GFF3181 0.60 -0.7 186,878 - GFF3181 0.60 -0.1 186,879 - GFF3181 0.60 -0.3 186,927 + GFF3181 0.63 +0.3 186,927 + GFF3181 0.63 +0.3
Or see this region's nucleotide sequence