Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3116 and GFF3117 are separated by 112 nucleotides GFF3117 and GFF3118 are separated by 6 nucleotides GFF3118 and GFF3119 overlap by 8 nucleotides GFF3119 and GFF3120 are separated by 121 nucleotides
GFF3116 - FIG00553873: hypothetical protein, at 123,715 to 124,599
GFF3116
GFF3117 - Biopolymer transport protein ExbD/TolR, at 124,712 to 125,137
GFF3117
GFF3118 - MotA/TolQ/ExbB proton channel family protein, at 125,144 to 125,878
GFF3118
GFF3119 - hypothetical protein, at 125,871 to 126,008
GFF3119
GFF3120 - Cystathionine beta-lyase (EC 4.4.1.8), at 126,130 to 127,317
GFF3120
Position (kb)
124
125
126 Strain fitness (log2 ratio)
-2
-1
0
1 at 123.725 kb on + strand at 123.726 kb on - strand at 124.075 kb on - strand, within GFF3116 at 124.149 kb on - strand, within GFF3116 at 124.272 kb on + strand, within GFF3116 at 124.339 kb on + strand, within GFF3116 at 124.340 kb on - strand, within GFF3116 at 124.519 kb on + strand at 124.520 kb on - strand at 124.520 kb on - strand at 124.520 kb on - strand at 124.525 kb on + strand at 124.525 kb on + strand at 124.526 kb on - strand at 124.549 kb on + strand at 124.550 kb on - strand at 124.593 kb on - strand at 124.615 kb on - strand at 124.869 kb on - strand, within GFF3117 at 124.912 kb on + strand, within GFF3117 at 124.945 kb on - strand, within GFF3117 at 124.981 kb on + strand, within GFF3117 at 125.175 kb on - strand at 125.232 kb on - strand, within GFF3118 at 125.265 kb on + strand, within GFF3118 at 125.266 kb on - strand, within GFF3118 at 125.359 kb on + strand, within GFF3118 at 125.423 kb on - strand, within GFF3118 at 125.570 kb on - strand, within GFF3118 at 125.570 kb on - strand, within GFF3118 at 125.715 kb on - strand, within GFF3118 at 125.833 kb on - strand at 125.869 kb on + strand at 125.916 kb on + strand, within GFF3119 at 125.917 kb on - strand, within GFF3119 at 125.917 kb on - strand, within GFF3119 at 125.917 kb on - strand, within GFF3119 at 125.920 kb on - strand, within GFF3119 at 125.920 kb on - strand, within GFF3119 at 125.920 kb on - strand, within GFF3119 at 125.960 kb on + strand, within GFF3119 at 125.960 kb on + strand, within GFF3119 at 125.968 kb on + strand, within GFF3119 at 125.982 kb on + strand, within GFF3119 at 126.029 kb on - strand at 126.079 kb on + strand at 126.081 kb on + strand at 126.091 kb on - strand at 126.091 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 123,725 + -1.0 123,726 - -0.2 124,075 - GFF3116 0.41 +0.4 124,149 - GFF3116 0.49 +0.4 124,272 + GFF3116 0.63 +0.6 124,339 + GFF3116 0.71 -0.1 124,340 - GFF3116 0.71 -0.4 124,519 + +0.1 124,520 - +1.7 124,520 - -0.8 124,520 - -0.1 124,525 + +0.1 124,525 + +0.5 124,526 - +0.1 124,549 + +0.2 124,550 - -1.3 124,593 - -0.0 124,615 - +0.7 124,869 - GFF3117 0.37 +1.5 124,912 + GFF3117 0.47 +0.8 124,945 - GFF3117 0.55 +0.2 124,981 + GFF3117 0.63 +0.5 125,175 - -0.7 125,232 - GFF3118 0.12 +0.4 125,265 + GFF3118 0.16 -0.1 125,266 - GFF3118 0.17 -0.2 125,359 + GFF3118 0.29 +0.0 125,423 - GFF3118 0.38 +0.7 125,570 - GFF3118 0.58 -2.3 125,570 - GFF3118 0.58 -0.2 125,715 - GFF3118 0.78 +0.7 125,833 - -0.3 125,869 + -0.5 125,916 + GFF3119 0.33 +0.2 125,917 - GFF3119 0.33 -0.2 125,917 - GFF3119 0.33 +0.3 125,917 - GFF3119 0.33 -0.0 125,920 - GFF3119 0.36 +0.2 125,920 - GFF3119 0.36 -0.0 125,920 - GFF3119 0.36 +0.5 125,960 + GFF3119 0.64 +0.1 125,960 + GFF3119 0.64 +0.4 125,968 + GFF3119 0.70 -0.2 125,982 + GFF3119 0.80 -0.2 126,029 - +0.1 126,079 + -0.1 126,081 + +0.1 126,091 - -0.2 126,091 - +0.3
Or see this region's nucleotide sequence