Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2821 and GFF2822 are separated by 71 nucleotides GFF2822 and GFF2823 are separated by 57 nucleotides GFF2823 and GFF2824 are separated by 130 nucleotides GFF2824 and GFF2825 are separated by 5 nucleotides GFF2825 and GFF2826 are separated by 80 nucleotides
GFF2821 - Starvation lipoprotein Slp paralog, at 342,381 to 342,962
GFF2821
GFF2822 - TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA, at 343,034 to 343,729
GFF2822
GFF2823 - DinG family ATP-dependent helicase YoaA, at 343,787 to 345,697
GFF2823
GFF2824 - RidA/YER057c/UK114 superfamily, group 2, YoaB-like protein, at 345,828 to 346,172
GFF2824
GFF2825 - FIG00638134: hypothetical protein, at 346,178 to 346,357
GFF2825
GFF2826 - Para-aminobenzoate synthase, aminase component (EC 2.6.1.85), at 346,438 to 347,802
GFF2826
Position (kb) 343
344
345
346 Strain fitness (log2 ratio) -1
0
1 at 342.812 kb on - strand, within GFF2821 at 342.939 kb on + strand at 342.940 kb on - strand at 343.026 kb on - strand at 343.744 kb on - strand at 343.769 kb on - strand at 344.072 kb on - strand, within GFF2823 at 344.107 kb on + strand, within GFF2823 at 344.414 kb on - strand, within GFF2823 at 344.415 kb on + strand, within GFF2823 at 344.415 kb on + strand, within GFF2823 at 344.617 kb on - strand, within GFF2823 at 344.652 kb on - strand, within GFF2823 at 344.674 kb on - strand, within GFF2823 at 344.676 kb on - strand, within GFF2823 at 344.772 kb on - strand, within GFF2823 at 345.099 kb on - strand, within GFF2823 at 345.305 kb on + strand, within GFF2823 at 345.333 kb on - strand, within GFF2823 at 345.534 kb on + strand at 345.535 kb on - strand at 345.535 kb on - strand at 345.540 kb on + strand at 345.540 kb on + strand at 345.541 kb on - strand at 345.726 kb on + strand at 345.727 kb on - strand at 345.746 kb on + strand at 345.822 kb on + strand at 345.822 kb on + strand at 345.823 kb on - strand at 345.826 kb on + strand at 345.826 kb on + strand at 345.827 kb on - strand at 345.880 kb on - strand, within GFF2824 at 345.884 kb on + strand, within GFF2824 at 346.112 kb on - strand, within GFF2824 at 346.276 kb on + strand, within GFF2825 at 346.276 kb on + strand, within GFF2825 at 346.362 kb on - strand at 346.372 kb on - strand at 346.390 kb on - strand at 346.417 kb on + strand at 346.638 kb on - strand, within GFF2826 at 346.641 kb on + strand, within GFF2826
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 342,812 - GFF2821 0.74 -0.1 342,939 + +0.1 342,940 - +1.1 343,026 - +0.2 343,744 - -1.7 343,769 - +0.1 344,072 - GFF2823 0.15 +0.1 344,107 + GFF2823 0.17 +1.3 344,414 - GFF2823 0.33 +0.4 344,415 + GFF2823 0.33 +0.5 344,415 + GFF2823 0.33 -0.9 344,617 - GFF2823 0.43 +0.7 344,652 - GFF2823 0.45 -0.6 344,674 - GFF2823 0.46 +0.5 344,676 - GFF2823 0.47 -1.6 344,772 - GFF2823 0.52 +0.5 345,099 - GFF2823 0.69 +1.1 345,305 + GFF2823 0.79 +0.0 345,333 - GFF2823 0.81 -0.0 345,534 + +0.6 345,535 - -1.1 345,535 - +0.4 345,540 + +0.7 345,540 + +0.1 345,541 - +1.7 345,726 + -0.2 345,727 - -0.3 345,746 + -0.0 345,822 + -0.4 345,822 + -0.1 345,823 - +0.4 345,826 + +0.6 345,826 + -0.9 345,827 - -0.1 345,880 - GFF2824 0.15 +0.5 345,884 + GFF2824 0.16 +0.1 346,112 - GFF2824 0.82 -0.7 346,276 + GFF2825 0.54 -1.3 346,276 + GFF2825 0.54 +0.1 346,362 - -0.1 346,372 - -0.1 346,390 - -0.3 346,417 + -0.1 346,638 - GFF2826 0.15 -1.5 346,641 + GFF2826 0.15 +0.5
Or see this region's nucleotide sequence