Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2485 and GFF2486 are separated by 40 nucleotides GFF2486 and GFF2487 are separated by 35 nucleotides GFF2487 and GFF2488 are separated by 152 nucleotides GFF2488 and GFF2489 are separated by 121 nucleotides
GFF2485 - Hypothetical UPF0257 lipoprotein ynfC precursor, at 5,609 to 6,286
GFF2485
GFF2486 - Spermidine N1-acetyltransferase (EC 2.3.1.57), at 6,327 to 6,887
GFF2486
GFF2487 - putative secreted protein, at 6,923 to 7,264
GFF2487
GFF2488 - Protein of unknown function UPF0060, at 7,417 to 7,743
GFF2488
GFF2489 - Starvation sensing protein RspA, at 7,865 to 9,079
GFF2489
Position (kb) 6
7
8 Strain fitness (log2 ratio) -1
0
1 at 6.052 kb on - strand, within GFF2485 at 6.064 kb on + strand, within GFF2485 at 6.116 kb on + strand, within GFF2485 at 6.254 kb on + strand at 6.255 kb on - strand at 6.255 kb on - strand at 6.526 kb on + strand, within GFF2486 at 6.527 kb on - strand, within GFF2486 at 6.527 kb on - strand, within GFF2486 at 6.608 kb on - strand, within GFF2486 at 6.620 kb on + strand, within GFF2486 at 6.815 kb on + strand, within GFF2486 at 6.816 kb on - strand, within GFF2486 at 6.816 kb on - strand, within GFF2486 at 6.875 kb on - strand at 6.888 kb on - strand at 6.888 kb on - strand at 6.911 kb on - strand at 6.914 kb on + strand at 7.201 kb on - strand, within GFF2487 at 7.202 kb on + strand, within GFF2487 at 7.202 kb on + strand, within GFF2487 at 7.202 kb on + strand, within GFF2487 at 7.202 kb on + strand, within GFF2487 at 7.255 kb on + strand at 7.256 kb on - strand at 7.595 kb on - strand, within GFF2488 at 7.614 kb on - strand, within GFF2488 at 7.614 kb on - strand, within GFF2488 at 7.615 kb on + strand, within GFF2488 at 7.615 kb on + strand, within GFF2488 at 7.615 kb on + strand, within GFF2488 at 7.615 kb on + strand, within GFF2488 at 7.616 kb on - strand, within GFF2488 at 7.616 kb on - strand, within GFF2488 at 7.616 kb on - strand, within GFF2488 at 7.616 kb on - strand, within GFF2488 at 7.616 kb on - strand, within GFF2488 at 7.737 kb on + strand at 7.738 kb on - strand at 7.741 kb on + strand at 7.742 kb on - strand at 7.909 kb on + strand at 8.001 kb on - strand, within GFF2489 at 8.222 kb on + strand, within GFF2489 at 8.223 kb on - strand, within GFF2489
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 6,052 - GFF2485 0.65 -0.1 6,064 + GFF2485 0.67 -0.0 6,116 + GFF2485 0.75 +0.6 6,254 + -0.8 6,255 - -0.0 6,255 - -0.1 6,526 + GFF2486 0.35 +0.7 6,527 - GFF2486 0.36 +0.3 6,527 - GFF2486 0.36 +0.1 6,608 - GFF2486 0.50 -0.2 6,620 + GFF2486 0.52 +0.2 6,815 + GFF2486 0.87 -0.1 6,816 - GFF2486 0.87 -0.5 6,816 - GFF2486 0.87 +0.7 6,875 - +0.3 6,888 - -0.2 6,888 - +0.3 6,911 - +0.3 6,914 + +0.8 7,201 - GFF2487 0.81 -0.5 7,202 + GFF2487 0.82 +0.2 7,202 + GFF2487 0.82 -0.0 7,202 + GFF2487 0.82 -0.8 7,202 + GFF2487 0.82 -0.5 7,255 + +0.3 7,256 - -0.1 7,595 - GFF2488 0.54 +0.3 7,614 - GFF2488 0.60 -0.2 7,614 - GFF2488 0.60 +0.2 7,615 + GFF2488 0.61 +0.4 7,615 + GFF2488 0.61 +0.3 7,615 + GFF2488 0.61 -0.1 7,615 + GFF2488 0.61 -0.4 7,616 - GFF2488 0.61 -0.3 7,616 - GFF2488 0.61 -0.4 7,616 - GFF2488 0.61 +0.9 7,616 - GFF2488 0.61 +0.2 7,616 - GFF2488 0.61 -0.0 7,737 + -0.2 7,738 - -0.2 7,741 + -0.2 7,742 - -1.0 7,909 + +0.3 8,001 - GFF2489 0.11 -0.4 8,222 + GFF2489 0.29 -0.5 8,223 - GFF2489 0.29 +0.2
Or see this region's nucleotide sequence