Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2332 and GFF2333 are separated by 11 nucleotides GFF2333 and GFF2334 are separated by 10 nucleotides
GFF2332 - Glycerol dehydrogenase (EC 1.1.1.6), at 121,627 to 122,730
GFF2332
GFF2333 - Transaldolase (EC 2.2.1.2), at 122,742 to 123,404
GFF2333
GFF2334 - Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9), at 123,415 to 125,904
GFF2334
Position (kb) 122
123
124 Strain fitness (log2 ratio) -1
0
1 at 121.788 kb on + strand, within GFF2332 at 121.789 kb on - strand, within GFF2332 at 121.923 kb on - strand, within GFF2332 at 121.926 kb on - strand, within GFF2332 at 121.926 kb on - strand, within GFF2332 at 122.109 kb on + strand, within GFF2332 at 122.111 kb on + strand, within GFF2332 at 122.112 kb on - strand, within GFF2332 at 122.117 kb on - strand, within GFF2332 at 122.145 kb on + strand, within GFF2332 at 122.340 kb on - strand, within GFF2332 at 122.344 kb on - strand, within GFF2332 at 122.374 kb on - strand, within GFF2332 at 122.554 kb on + strand, within GFF2332 at 122.661 kb on - strand at 122.698 kb on - strand at 122.699 kb on + strand at 122.700 kb on - strand at 122.703 kb on + strand at 122.731 kb on - strand at 122.743 kb on + strand at 122.743 kb on + strand at 122.793 kb on + strand at 122.806 kb on - strand at 122.816 kb on + strand, within GFF2333 at 122.817 kb on - strand, within GFF2333 at 122.836 kb on - strand, within GFF2333 at 122.841 kb on + strand, within GFF2333 at 122.842 kb on - strand, within GFF2333 at 122.853 kb on - strand, within GFF2333 at 122.903 kb on + strand, within GFF2333 at 122.919 kb on + strand, within GFF2333 at 122.973 kb on - strand, within GFF2333 at 123.237 kb on + strand, within GFF2333 at 123.237 kb on + strand, within GFF2333 at 123.291 kb on - strand, within GFF2333 at 123.404 kb on + strand at 123.416 kb on + strand at 123.534 kb on + strand at 123.534 kb on + strand at 123.534 kb on + strand at 123.534 kb on + strand at 123.561 kb on + strand at 123.573 kb on + strand at 123.574 kb on - strand at 123.574 kb on - strand at 123.652 kb on - strand at 124.033 kb on + strand, within GFF2334 at 124.224 kb on + strand, within GFF2334 at 124.230 kb on + strand, within GFF2334 at 124.231 kb on - strand, within GFF2334 at 124.297 kb on - strand, within GFF2334 at 124.329 kb on + strand, within GFF2334 at 124.330 kb on - strand, within GFF2334 at 124.330 kb on - strand, within GFF2334 at 124.396 kb on - strand, within GFF2334
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 121,788 + GFF2332 0.15 +0.3 121,789 - GFF2332 0.15 +0.1 121,923 - GFF2332 0.27 -0.4 121,926 - GFF2332 0.27 -0.3 121,926 - GFF2332 0.27 +0.1 122,109 + GFF2332 0.44 +0.9 122,111 + GFF2332 0.44 -0.3 122,112 - GFF2332 0.44 -0.3 122,117 - GFF2332 0.44 -0.6 122,145 + GFF2332 0.47 +0.1 122,340 - GFF2332 0.65 -0.4 122,344 - GFF2332 0.65 -1.0 122,374 - GFF2332 0.68 +1.6 122,554 + GFF2332 0.84 +0.2 122,661 - +0.6 122,698 - +0.0 122,699 + -1.1 122,700 - +0.2 122,703 + -0.3 122,731 - -0.2 122,743 + +0.1 122,743 + -0.2 122,793 + -0.6 122,806 - -0.2 122,816 + GFF2333 0.11 +1.0 122,817 - GFF2333 0.11 -0.1 122,836 - GFF2333 0.14 -0.5 122,841 + GFF2333 0.15 +1.4 122,842 - GFF2333 0.15 +0.1 122,853 - GFF2333 0.17 +0.2 122,903 + GFF2333 0.24 -0.1 122,919 + GFF2333 0.27 -0.4 122,973 - GFF2333 0.35 +0.1 123,237 + GFF2333 0.75 +0.7 123,237 + GFF2333 0.75 +0.3 123,291 - GFF2333 0.83 +0.8 123,404 + +0.1 123,416 + +0.3 123,534 + -0.5 123,534 + +0.8 123,534 + +0.4 123,534 + +0.1 123,561 + -0.0 123,573 + -0.1 123,574 - -0.4 123,574 - -0.9 123,652 - -0.4 124,033 + GFF2334 0.25 -1.0 124,224 + GFF2334 0.32 +0.2 124,230 + GFF2334 0.33 -0.3 124,231 - GFF2334 0.33 -1.2 124,297 - GFF2334 0.35 -0.9 124,329 + GFF2334 0.37 -0.3 124,330 - GFF2334 0.37 -0.1 124,330 - GFF2334 0.37 -0.0 124,396 - GFF2334 0.39 -0.5
Or see this region's nucleotide sequence