Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF21 and GFF22 are separated by 7 nucleotides GFF22 and GFF23 are separated by 38 nucleotides GFF23 and GFF24 are separated by 391 nucleotides GFF24 and GFF25 overlap by 37 nucleotides
GFF21 - Methionine ABC transporter ATP-binding protein, at 1,035 to 2,066
GFF21
GFF22 - Methionine ABC transporter permease protein, at 2,074 to 2,712
GFF22
GFF23 - Methionine ABC transporter substrate-binding protein, at 2,751 to 3,566
GFF23
GFF24 - Protein RcsF, at 3,958 to 4,089
GFF24
GFF25 - COG1720: Uncharacterized conserved protein, at 4,053 to 4,793
GFF25
Position (kb)
2
3
4 Strain fitness (log2 ratio)
-1
0
1 at 1.920 kb on - strand, within GFF21 at 1.922 kb on + strand, within GFF21 at 1.923 kb on - strand, within GFF21 at 1.968 kb on - strand at 2.044 kb on + strand at 2.050 kb on + strand at 2.173 kb on + strand, within GFF22 at 2.174 kb on - strand, within GFF22 at 2.197 kb on - strand, within GFF22 at 2.263 kb on - strand, within GFF22 at 2.339 kb on + strand, within GFF22 at 2.376 kb on + strand, within GFF22 at 2.396 kb on + strand, within GFF22 at 2.515 kb on - strand, within GFF22 at 2.594 kb on - strand, within GFF22 at 2.609 kb on - strand, within GFF22 at 2.609 kb on - strand, within GFF22 at 2.628 kb on + strand, within GFF22 at 2.707 kb on - strand at 2.731 kb on + strand at 2.849 kb on + strand, within GFF23 at 2.949 kb on - strand, within GFF23 at 3.363 kb on + strand, within GFF23 at 3.364 kb on - strand, within GFF23 at 3.592 kb on - strand at 3.631 kb on - strand at 3.651 kb on - strand at 3.681 kb on + strand at 3.740 kb on + strand at 3.839 kb on - strand at 3.957 kb on + strand at 3.957 kb on + strand at 3.958 kb on - strand at 4.209 kb on + strand, within GFF25 at 4.405 kb on + strand, within GFF25 at 4.490 kb on + strand, within GFF25
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 1,920 - GFF21 0.86 -0.8 1,922 + GFF21 0.86 +0.5 1,923 - GFF21 0.86 +0.1 1,968 - -0.9 2,044 + +0.3 2,050 + -0.4 2,173 + GFF22 0.15 +0.0 2,174 - GFF22 0.16 +0.0 2,197 - GFF22 0.19 -0.2 2,263 - GFF22 0.30 +0.2 2,339 + GFF22 0.41 +0.5 2,376 + GFF22 0.47 -0.3 2,396 + GFF22 0.50 +0.2 2,515 - GFF22 0.69 +0.0 2,594 - GFF22 0.81 +0.2 2,609 - GFF22 0.84 -0.1 2,609 - GFF22 0.84 -0.0 2,628 + GFF22 0.87 +0.1 2,707 - +0.6 2,731 + -0.1 2,849 + GFF23 0.12 -0.5 2,949 - GFF23 0.24 -0.3 3,363 + GFF23 0.75 -0.3 3,364 - GFF23 0.75 -1.6 3,592 - +0.3 3,631 - -0.4 3,651 - +0.2 3,681 + -0.4 3,740 + -0.8 3,839 - -0.9 3,957 + +0.4 3,957 + +0.0 3,958 - -0.1 4,209 + GFF25 0.21 -0.4 4,405 + GFF25 0.48 +0.2 4,490 + GFF25 0.59 -0.1
Or see this region's nucleotide sequence