Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2273 and GFF2274 overlap by 8 nucleotides GFF2274 and GFF2275 are separated by 18 nucleotides GFF2275 and GFF2276 are separated by 328 nucleotides
GFF2273 - Predicted rhamnogalacturonide-specific TRAP-type transporter, large transmembrane component RhiC, at 62,317 to 63,624
GFF2273
GFF2274 - Predicted rhamnogalacturonide-specific TRAP-type transporter, small transmembrane component RhiB, at 63,617 to 64,132
GFF2274
GFF2275 - Predicted rhamnogalacturonide-specific TRAP-type transporter, substrate-binding component RhiA, at 64,151 to 65,134
GFF2275
GFF2276 - Manganese superoxide dismutase (EC 1.15.1.1), at 65,463 to 66,083
GFF2276
Position (kb) 64
65
66 Strain fitness (log2 ratio) -2
-1
0
1 at 63.301 kb on - strand, within GFF2273 at 63.319 kb on - strand, within GFF2273 at 63.321 kb on - strand, within GFF2273 at 63.321 kb on - strand, within GFF2273 at 63.321 kb on - strand, within GFF2273 at 63.592 kb on + strand at 63.592 kb on + strand at 63.598 kb on - strand at 63.613 kb on - strand at 63.705 kb on + strand, within GFF2274 at 63.705 kb on + strand, within GFF2274 at 63.925 kb on + strand, within GFF2274 at 63.926 kb on - strand, within GFF2274 at 63.926 kb on - strand, within GFF2274 at 63.926 kb on - strand, within GFF2274 at 64.104 kb on - strand at 64.148 kb on - strand at 64.286 kb on + strand, within GFF2275 at 64.412 kb on - strand, within GFF2275 at 64.458 kb on + strand, within GFF2275 at 64.459 kb on - strand, within GFF2275 at 64.539 kb on - strand, within GFF2275 at 64.539 kb on - strand, within GFF2275 at 64.546 kb on + strand, within GFF2275 at 64.546 kb on + strand, within GFF2275 at 64.547 kb on - strand, within GFF2275 at 64.547 kb on - strand, within GFF2275 at 64.548 kb on + strand, within GFF2275 at 64.548 kb on + strand, within GFF2275 at 64.549 kb on - strand, within GFF2275 at 64.646 kb on + strand, within GFF2275 at 64.682 kb on - strand, within GFF2275 at 64.715 kb on + strand, within GFF2275 at 64.749 kb on + strand, within GFF2275 at 64.877 kb on - strand, within GFF2275 at 64.951 kb on - strand, within GFF2275 at 64.951 kb on - strand, within GFF2275 at 64.955 kb on + strand, within GFF2275 at 64.956 kb on - strand, within GFF2275 at 65.094 kb on - strand at 65.115 kb on - strand at 65.115 kb on - strand at 65.117 kb on - strand at 65.402 kb on + strand at 65.462 kb on + strand at 65.469 kb on + strand at 65.471 kb on + strand at 65.472 kb on - strand at 65.565 kb on + strand, within GFF2276 at 65.566 kb on - strand, within GFF2276 at 65.985 kb on + strand, within GFF2276 at 65.986 kb on - strand, within GFF2276
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 63,301 - GFF2273 0.75 +0.8 63,319 - GFF2273 0.77 +0.0 63,321 - GFF2273 0.77 +0.4 63,321 - GFF2273 0.77 +0.1 63,321 - GFF2273 0.77 +0.0 63,592 + +0.4 63,592 + +0.1 63,598 - -0.6 63,613 - +0.0 63,705 + GFF2274 0.17 +0.9 63,705 + GFF2274 0.17 +0.0 63,925 + GFF2274 0.60 +0.0 63,926 - GFF2274 0.60 -2.6 63,926 - GFF2274 0.60 -1.1 63,926 - GFF2274 0.60 +0.3 64,104 - +0.2 64,148 - -0.2 64,286 + GFF2275 0.14 +0.0 64,412 - GFF2275 0.27 -1.0 64,458 + GFF2275 0.31 -0.1 64,459 - GFF2275 0.31 +0.4 64,539 - GFF2275 0.39 +0.1 64,539 - GFF2275 0.39 -0.1 64,546 + GFF2275 0.40 +0.1 64,546 + GFF2275 0.40 +0.1 64,547 - GFF2275 0.40 +0.4 64,547 - GFF2275 0.40 +0.1 64,548 + GFF2275 0.40 +0.3 64,548 + GFF2275 0.40 +0.2 64,549 - GFF2275 0.40 -0.1 64,646 + GFF2275 0.50 +0.5 64,682 - GFF2275 0.54 -0.6 64,715 + GFF2275 0.57 +0.4 64,749 + GFF2275 0.61 -0.3 64,877 - GFF2275 0.74 +0.4 64,951 - GFF2275 0.81 +0.0 64,951 - GFF2275 0.81 +0.7 64,955 + GFF2275 0.82 -0.0 64,956 - GFF2275 0.82 +1.2 65,094 - +0.8 65,115 - +0.7 65,115 - -0.1 65,117 - -0.1 65,402 + +0.4 65,462 + -0.5 65,469 + +0.4 65,471 + +0.1 65,472 - -0.3 65,565 + GFF2276 0.16 -1.2 65,566 - GFF2276 0.17 -1.0 65,985 + GFF2276 0.84 -0.9 65,986 - GFF2276 0.84 -0.7
Or see this region's nucleotide sequence