Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2251 and GFF2252 overlap by 4 nucleotides GFF2252 and GFF2253 are separated by 12 nucleotides GFF2253 and GFF2254 are separated by 320 nucleotides
GFF2251 - Formate dehydrogenase O gamma subunit (EC 1.2.1.2), at 42,135 to 42,770
GFF2251
GFF2252 - Formate dehydrogenase O beta subunit (EC 1.2.1.2), at 42,767 to 43,669
GFF2252
GFF2253 - Formate dehydrogenase O alpha subunit (EC 1.2.1.2) @ selenocysteine-containing, at 43,682 to 46,732
GFF2253
GFF2254 - Formate dehydrogenase chain D (EC 1.2.1.2), at 47,053 to 47,763
GFF2254
Position (kb)
43
44
45
46
47 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 42.740 kb on + strand at 42.740 kb on + strand at 42.742 kb on + strand at 42.844 kb on - strand at 42.993 kb on + strand, within GFF2252 at 42.994 kb on - strand, within GFF2252 at 42.994 kb on - strand, within GFF2252 at 42.994 kb on - strand, within GFF2252 at 43.061 kb on + strand, within GFF2252 at 43.189 kb on + strand, within GFF2252 at 43.190 kb on - strand, within GFF2252 at 43.190 kb on - strand, within GFF2252 at 43.190 kb on - strand, within GFF2252 at 43.195 kb on + strand, within GFF2252 at 43.252 kb on + strand, within GFF2252 at 43.253 kb on - strand, within GFF2252 at 43.270 kb on + strand, within GFF2252 at 43.369 kb on + strand, within GFF2252 at 43.370 kb on - strand, within GFF2252 at 43.454 kb on + strand, within GFF2252 at 43.474 kb on - strand, within GFF2252 at 43.670 kb on - strand at 43.670 kb on - strand at 43.861 kb on + strand at 43.891 kb on + strand at 43.891 kb on + strand at 43.892 kb on - strand at 43.893 kb on + strand at 43.893 kb on + strand at 43.945 kb on + strand at 43.946 kb on - strand at 44.181 kb on + strand, within GFF2253 at 44.212 kb on + strand, within GFF2253 at 44.395 kb on - strand, within GFF2253 at 44.636 kb on - strand, within GFF2253 at 44.676 kb on + strand, within GFF2253 at 44.688 kb on + strand, within GFF2253 at 44.742 kb on + strand, within GFF2253 at 44.742 kb on + strand, within GFF2253 at 44.759 kb on + strand, within GFF2253 at 44.760 kb on - strand, within GFF2253 at 44.760 kb on - strand, within GFF2253 at 44.834 kb on + strand, within GFF2253 at 44.835 kb on - strand, within GFF2253 at 45.001 kb on - strand, within GFF2253 at 45.078 kb on + strand, within GFF2253 at 45.122 kb on + strand, within GFF2253 at 45.334 kb on - strand, within GFF2253 at 45.363 kb on - strand, within GFF2253 at 45.461 kb on - strand, within GFF2253 at 45.464 kb on - strand, within GFF2253 at 45.464 kb on - strand, within GFF2253 at 45.556 kb on - strand, within GFF2253 at 45.679 kb on + strand, within GFF2253 at 45.715 kb on - strand, within GFF2253 at 45.715 kb on - strand, within GFF2253 at 45.736 kb on - strand, within GFF2253 at 45.756 kb on + strand, within GFF2253 at 45.757 kb on - strand, within GFF2253 at 45.832 kb on - strand, within GFF2253 at 45.832 kb on - strand, within GFF2253 at 45.864 kb on + strand, within GFF2253 at 45.865 kb on - strand, within GFF2253 at 45.865 kb on - strand, within GFF2253 at 45.908 kb on + strand, within GFF2253 at 46.223 kb on - strand, within GFF2253 at 46.331 kb on - strand, within GFF2253 at 46.376 kb on - strand, within GFF2253 at 46.429 kb on + strand at 46.511 kb on - strand at 46.620 kb on + strand at 46.877 kb on - strand at 46.885 kb on - strand at 46.911 kb on - strand at 46.911 kb on - strand at 46.911 kb on - strand at 46.911 kb on - strand at 46.912 kb on + strand at 46.913 kb on - strand at 46.935 kb on + strand at 46.935 kb on + strand at 46.936 kb on - strand at 46.937 kb on + strand at 46.956 kb on - strand at 47.065 kb on + strand at 47.066 kb on - strand at 47.066 kb on - strand at 47.066 kb on - strand at 47.294 kb on + strand, within GFF2254 at 47.294 kb on + strand, within GFF2254 at 47.295 kb on - strand, within GFF2254 at 47.325 kb on + strand, within GFF2254 at 47.433 kb on + strand, within GFF2254 at 47.514 kb on + strand, within GFF2254 at 47.591 kb on - strand, within GFF2254 at 47.709 kb on + strand at 47.731 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 42,740 + +0.1 42,740 + -0.2 42,742 + -0.4 42,844 - -1.0 42,993 + GFF2252 0.25 -0.6 42,994 - GFF2252 0.25 -1.0 42,994 - GFF2252 0.25 -0.5 42,994 - GFF2252 0.25 -1.0 43,061 + GFF2252 0.33 -0.3 43,189 + GFF2252 0.47 -0.3 43,190 - GFF2252 0.47 +0.8 43,190 - GFF2252 0.47 +0.2 43,190 - GFF2252 0.47 -0.3 43,195 + GFF2252 0.47 -0.0 43,252 + GFF2252 0.54 +0.7 43,253 - GFF2252 0.54 -0.4 43,270 + GFF2252 0.56 +0.5 43,369 + GFF2252 0.67 -0.1 43,370 - GFF2252 0.67 +0.0 43,454 + GFF2252 0.76 -0.6 43,474 - GFF2252 0.78 -0.1 43,670 - +0.0 43,670 - +0.2 43,861 + -0.2 43,891 + +0.9 43,891 + -0.5 43,892 - -0.4 43,893 + +0.5 43,893 + -1.1 43,945 + +0.0 43,946 - +0.0 44,181 + GFF2253 0.16 -0.0 44,212 + GFF2253 0.17 +0.7 44,395 - GFF2253 0.23 +0.4 44,636 - GFF2253 0.31 +0.2 44,676 + GFF2253 0.33 -0.2 44,688 + GFF2253 0.33 -0.8 44,742 + GFF2253 0.35 +0.1 44,742 + GFF2253 0.35 +0.9 44,759 + GFF2253 0.35 -0.8 44,760 - GFF2253 0.35 -0.5 44,760 - GFF2253 0.35 -0.0 44,834 + GFF2253 0.38 +0.1 44,835 - GFF2253 0.38 +0.2 45,001 - GFF2253 0.43 +0.6 45,078 + GFF2253 0.46 -0.0 45,122 + GFF2253 0.47 -0.1 45,334 - GFF2253 0.54 -2.4 45,363 - GFF2253 0.55 +0.3 45,461 - GFF2253 0.58 +0.0 45,464 - GFF2253 0.58 -0.1 45,464 - GFF2253 0.58 -0.8 45,556 - GFF2253 0.61 -0.0 45,679 + GFF2253 0.65 -0.1 45,715 - GFF2253 0.67 -0.0 45,715 - GFF2253 0.67 +0.3 45,736 - GFF2253 0.67 -2.5 45,756 + GFF2253 0.68 +0.4 45,757 - GFF2253 0.68 -0.1 45,832 - GFF2253 0.70 +0.1 45,832 - GFF2253 0.70 +0.6 45,864 + GFF2253 0.72 -0.0 45,865 - GFF2253 0.72 -0.3 45,865 - GFF2253 0.72 +0.0 45,908 + GFF2253 0.73 +0.2 46,223 - GFF2253 0.83 +0.2 46,331 - GFF2253 0.87 +1.9 46,376 - GFF2253 0.88 +0.5 46,429 + -0.0 46,511 - +0.4 46,620 + -0.9 46,877 - -0.5 46,885 - +0.1 46,911 - +0.0 46,911 - -0.5 46,911 - +0.1 46,911 - +0.2 46,912 + -0.4 46,913 - -0.2 46,935 + -0.2 46,935 + -0.2 46,936 - -0.4 46,937 + +0.1 46,956 - -0.3 47,065 + -0.0 47,066 - -1.1 47,066 - -0.2 47,066 - -0.6 47,294 + GFF2254 0.34 -0.3 47,294 + GFF2254 0.34 -1.6 47,295 - GFF2254 0.34 +0.4 47,325 + GFF2254 0.38 -0.2 47,433 + GFF2254 0.53 +2.6 47,514 + GFF2254 0.65 -0.9 47,591 - GFF2254 0.76 -1.3 47,709 + +0.1 47,731 + -0.4
Or see this region's nucleotide sequence