Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2249 and GFF2250 are separated by 46 nucleotides GFF2250 and GFF2251 overlap by 4 nucleotides GFF2251 and GFF2252 overlap by 4 nucleotides GFF2252 and GFF2253 are separated by 12 nucleotides
GFF2249 - Putative merR family bacterial regulatory protein, at 40,872 to 41,162
GFF2249
GFF2250 - formate dehydrogenase formation protein FdhE, at 41,209 to 42,138
GFF2250
GFF2251 - Formate dehydrogenase O gamma subunit (EC 1.2.1.2), at 42,135 to 42,770
GFF2251
GFF2252 - Formate dehydrogenase O beta subunit (EC 1.2.1.2), at 42,767 to 43,669
GFF2252
GFF2253 - Formate dehydrogenase O alpha subunit (EC 1.2.1.2) @ selenocysteine-containing, at 43,682 to 46,732
GFF2253
Position (kb) 42
43 Strain fitness (log2 ratio) -1
0
1 at 41.143 kb on + strand at 41.144 kb on - strand at 41.144 kb on - strand at 41.181 kb on + strand at 41.357 kb on + strand, within GFF2250 at 41.357 kb on + strand, within GFF2250 at 41.425 kb on + strand, within GFF2250 at 41.426 kb on - strand, within GFF2250 at 41.494 kb on - strand, within GFF2250 at 41.573 kb on + strand, within GFF2250 at 41.662 kb on - strand, within GFF2250 at 41.798 kb on - strand, within GFF2250 at 41.838 kb on + strand, within GFF2250 at 41.899 kb on - strand, within GFF2250 at 41.933 kb on + strand, within GFF2250 at 42.038 kb on + strand, within GFF2250 at 42.039 kb on - strand, within GFF2250 at 42.125 kb on - strand at 42.236 kb on + strand, within GFF2251 at 42.269 kb on + strand, within GFF2251 at 42.270 kb on - strand, within GFF2251 at 42.270 kb on - strand, within GFF2251 at 42.270 kb on - strand, within GFF2251 at 42.270 kb on - strand, within GFF2251 at 42.291 kb on + strand, within GFF2251 at 42.291 kb on + strand, within GFF2251 at 42.381 kb on + strand, within GFF2251 at 42.582 kb on - strand, within GFF2251 at 42.740 kb on + strand at 42.740 kb on + strand at 42.742 kb on + strand at 42.844 kb on - strand at 42.993 kb on + strand, within GFF2252 at 42.994 kb on - strand, within GFF2252 at 42.994 kb on - strand, within GFF2252 at 42.994 kb on - strand, within GFF2252 at 43.061 kb on + strand, within GFF2252 at 43.189 kb on + strand, within GFF2252 at 43.190 kb on - strand, within GFF2252 at 43.190 kb on - strand, within GFF2252 at 43.190 kb on - strand, within GFF2252 at 43.195 kb on + strand, within GFF2252 at 43.252 kb on + strand, within GFF2252 at 43.253 kb on - strand, within GFF2252 at 43.270 kb on + strand, within GFF2252 at 43.369 kb on + strand, within GFF2252 at 43.370 kb on - strand, within GFF2252 at 43.454 kb on + strand, within GFF2252 at 43.474 kb on - strand, within GFF2252 at 43.670 kb on - strand at 43.670 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 41,143 + -1.0 41,144 - -0.2 41,144 - +0.1 41,181 + +0.4 41,357 + GFF2250 0.16 -0.3 41,357 + GFF2250 0.16 -0.8 41,425 + GFF2250 0.23 -1.6 41,426 - GFF2250 0.23 -0.6 41,494 - GFF2250 0.31 +0.0 41,573 + GFF2250 0.39 -0.3 41,662 - GFF2250 0.49 -0.6 41,798 - GFF2250 0.63 +0.7 41,838 + GFF2250 0.68 -0.4 41,899 - GFF2250 0.74 +0.6 41,933 + GFF2250 0.78 -0.3 42,038 + GFF2250 0.89 -0.7 42,039 - GFF2250 0.89 -0.1 42,125 - +0.2 42,236 + GFF2251 0.16 +0.2 42,269 + GFF2251 0.21 +0.0 42,270 - GFF2251 0.21 +0.1 42,270 - GFF2251 0.21 +0.2 42,270 - GFF2251 0.21 +0.0 42,270 - GFF2251 0.21 -0.1 42,291 + GFF2251 0.25 +0.3 42,291 + GFF2251 0.25 +0.2 42,381 + GFF2251 0.39 +0.2 42,582 - GFF2251 0.70 +0.4 42,740 + +0.1 42,740 + -0.2 42,742 + -0.4 42,844 - -1.0 42,993 + GFF2252 0.25 -0.6 42,994 - GFF2252 0.25 -1.0 42,994 - GFF2252 0.25 -0.5 42,994 - GFF2252 0.25 -1.0 43,061 + GFF2252 0.33 -0.3 43,189 + GFF2252 0.47 -0.3 43,190 - GFF2252 0.47 +0.8 43,190 - GFF2252 0.47 +0.2 43,190 - GFF2252 0.47 -0.3 43,195 + GFF2252 0.47 -0.0 43,252 + GFF2252 0.54 +0.7 43,253 - GFF2252 0.54 -0.4 43,270 + GFF2252 0.56 +0.5 43,369 + GFF2252 0.67 -0.1 43,370 - GFF2252 0.67 +0.0 43,454 + GFF2252 0.76 -0.6 43,474 - GFF2252 0.78 -0.1 43,670 - +0.0 43,670 - +0.2
Or see this region's nucleotide sequence