Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2186 and GFF2187 are separated by 3 nucleotides GFF2187 and GFF2188 are separated by 573 nucleotides GFF2188 and GFF2189 are separated by 184 nucleotides
GFF2186 - N-succinyl-L,L-diaminopimelate desuccinylase (EC 3.5.1.18), at 32,563 to 33,690
GFF2186
GFF2187 - FIG138056: a glutathione-dependent thiol reductase, at 33,694 to 34,050
GFF2187
GFF2188 - RND efflux system, inner membrane transporter CmeB, at 34,624 to 37,737
GFF2188
GFF2189 - Nitrate/nitrite sensor protein (EC 2.7.3.-), at 37,922 to 39,622
GFF2189
Position (kb) 34
35
36
37
38 Strain fitness (log2 ratio) -1
0
1 at 33.720 kb on - strand at 33.921 kb on + strand, within GFF2187 at 33.940 kb on + strand, within GFF2187 at 34.322 kb on - strand at 34.416 kb on + strand at 34.428 kb on + strand at 34.437 kb on + strand at 34.789 kb on + strand at 34.967 kb on + strand, within GFF2188 at 35.069 kb on + strand, within GFF2188 at 35.288 kb on + strand, within GFF2188 at 35.288 kb on + strand, within GFF2188 at 35.376 kb on - strand, within GFF2188 at 35.376 kb on - strand, within GFF2188 at 35.404 kb on - strand, within GFF2188 at 35.408 kb on + strand, within GFF2188 at 35.429 kb on + strand, within GFF2188 at 35.429 kb on + strand, within GFF2188 at 35.430 kb on - strand, within GFF2188 at 35.430 kb on - strand, within GFF2188 at 35.430 kb on - strand, within GFF2188 at 35.430 kb on - strand, within GFF2188 at 35.458 kb on - strand, within GFF2188 at 35.471 kb on + strand, within GFF2188 at 36.021 kb on + strand, within GFF2188 at 36.021 kb on + strand, within GFF2188 at 36.108 kb on - strand, within GFF2188 at 36.247 kb on + strand, within GFF2188 at 36.248 kb on - strand, within GFF2188 at 36.251 kb on - strand, within GFF2188 at 36.339 kb on - strand, within GFF2188 at 36.524 kb on - strand, within GFF2188 at 36.571 kb on - strand, within GFF2188 at 36.588 kb on - strand, within GFF2188 at 36.614 kb on - strand, within GFF2188 at 36.671 kb on + strand, within GFF2188 at 36.672 kb on - strand, within GFF2188 at 36.672 kb on - strand, within GFF2188 at 36.770 kb on + strand, within GFF2188 at 36.915 kb on - strand, within GFF2188 at 37.035 kb on + strand, within GFF2188 at 37.194 kb on - strand, within GFF2188 at 37.194 kb on - strand, within GFF2188 at 37.194 kb on - strand, within GFF2188 at 37.194 kb on - strand, within GFF2188 at 37.205 kb on + strand, within GFF2188 at 37.294 kb on - strand, within GFF2188 at 37.508 kb on + strand at 37.509 kb on - strand at 37.509 kb on - strand at 37.605 kb on + strand at 37.610 kb on - strand at 37.634 kb on + strand at 37.763 kb on + strand at 37.764 kb on - strand at 37.833 kb on + strand at 37.838 kb on + strand at 37.923 kb on + strand at 37.966 kb on + strand at 38.042 kb on + strand at 38.043 kb on - strand at 38.108 kb on - strand, within GFF2189 at 38.488 kb on - strand, within GFF2189 at 38.496 kb on - strand, within GFF2189 at 38.589 kb on + strand, within GFF2189 at 38.644 kb on + strand, within GFF2189
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 33,720 - -0.5 33,921 + GFF2187 0.64 +0.6 33,940 + GFF2187 0.69 +0.9 34,322 - +0.1 34,416 + +0.0 34,428 + -0.3 34,437 + +1.1 34,789 + -1.6 34,967 + GFF2188 0.11 -0.9 35,069 + GFF2188 0.14 -0.4 35,288 + GFF2188 0.21 -0.7 35,288 + GFF2188 0.21 -0.1 35,376 - GFF2188 0.24 +1.2 35,376 - GFF2188 0.24 +0.9 35,404 - GFF2188 0.25 -0.1 35,408 + GFF2188 0.25 -0.2 35,429 + GFF2188 0.26 +0.4 35,429 + GFF2188 0.26 +0.2 35,430 - GFF2188 0.26 +0.9 35,430 - GFF2188 0.26 +0.6 35,430 - GFF2188 0.26 -1.4 35,430 - GFF2188 0.26 +1.1 35,458 - GFF2188 0.27 +0.4 35,471 + GFF2188 0.27 -0.1 36,021 + GFF2188 0.45 +0.8 36,021 + GFF2188 0.45 -0.7 36,108 - GFF2188 0.48 +0.2 36,247 + GFF2188 0.52 -0.3 36,248 - GFF2188 0.52 -0.0 36,251 - GFF2188 0.52 +1.4 36,339 - GFF2188 0.55 +0.0 36,524 - GFF2188 0.61 -0.7 36,571 - GFF2188 0.63 +0.7 36,588 - GFF2188 0.63 -0.0 36,614 - GFF2188 0.64 -1.4 36,671 + GFF2188 0.66 +1.5 36,672 - GFF2188 0.66 +0.4 36,672 - GFF2188 0.66 -0.1 36,770 + GFF2188 0.69 +0.2 36,915 - GFF2188 0.74 -0.2 37,035 + GFF2188 0.77 +1.2 37,194 - GFF2188 0.83 -0.0 37,194 - GFF2188 0.83 +0.2 37,194 - GFF2188 0.83 -0.1 37,194 - GFF2188 0.83 -0.1 37,205 + GFF2188 0.83 +0.2 37,294 - GFF2188 0.86 +0.0 37,508 + -0.2 37,509 - -0.7 37,509 - +0.6 37,605 + -0.0 37,610 - -1.0 37,634 + -0.2 37,763 + -0.1 37,764 - +0.2 37,833 + +0.2 37,838 + -1.0 37,923 + -1.2 37,966 + +0.1 38,042 + -0.2 38,043 - +0.3 38,108 - GFF2189 0.11 +0.3 38,488 - GFF2189 0.33 -0.3 38,496 - GFF2189 0.34 +0.1 38,589 + GFF2189 0.39 -0.3 38,644 + GFF2189 0.42 -0.3
Or see this region's nucleotide sequence