Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1996 and GFF1997 overlap by 8 nucleotides GFF1997 and GFF1998 are separated by 10 nucleotides GFF1998 and GFF1999 are separated by 10 nucleotides GFF1999 and GFF2000 are separated by 111 nucleotides
GFF1996 - Succinate dehydrogenase flavoprotein subunit (EC 1.3.99.1), at 10,702 to 12,492
GFF1996
GFF1997 - Succinate dehydrogenase iron-sulfur protein (EC 1.3.99.1), at 12,485 to 13,219
GFF1997
GFF1998 - Fumarate reductase subunit C, at 13,230 to 13,625
GFF1998
GFF1999 - Fumarate reductase subunit D, at 13,636 to 13,995
GFF1999
GFF2000 - Outer membrane lipoprotein Blc, at 14,107 to 14,640
GFF2000
Position (kb) 12
13
14 Strain fitness (log2 ratio) -1
0
1
2 at 11.548 kb on - strand, within GFF1996 at 11.716 kb on + strand, within GFF1996 at 11.770 kb on + strand, within GFF1996 at 11.771 kb on - strand, within GFF1996 at 11.771 kb on - strand, within GFF1996 at 12.100 kb on + strand, within GFF1996 at 12.100 kb on + strand, within GFF1996 at 12.136 kb on - strand, within GFF1996 at 12.170 kb on + strand, within GFF1996 at 12.171 kb on - strand, within GFF1996 at 12.214 kb on + strand, within GFF1996 at 12.214 kb on + strand, within GFF1996 at 12.215 kb on - strand, within GFF1996 at 12.217 kb on - strand, within GFF1996 at 12.255 kb on + strand, within GFF1996 at 12.255 kb on + strand, within GFF1996 at 12.469 kb on - strand at 12.611 kb on + strand, within GFF1997 at 12.611 kb on + strand, within GFF1997 at 12.738 kb on - strand, within GFF1997 at 12.842 kb on + strand, within GFF1997 at 12.842 kb on + strand, within GFF1997 at 12.847 kb on + strand, within GFF1997 at 12.847 kb on + strand, within GFF1997 at 12.951 kb on - strand, within GFF1997 at 13.238 kb on + strand at 13.251 kb on + strand at 13.251 kb on + strand at 13.252 kb on - strand at 13.252 kb on - strand at 13.305 kb on + strand, within GFF1998 at 13.306 kb on - strand, within GFF1998 at 13.307 kb on + strand, within GFF1998 at 13.308 kb on - strand, within GFF1998 at 13.594 kb on + strand at 13.606 kb on - strand at 13.737 kb on - strand, within GFF1999 at 13.767 kb on - strand, within GFF1999 at 13.874 kb on + strand, within GFF1999 at 13.939 kb on + strand, within GFF1999 at 13.993 kb on + strand at 14.014 kb on + strand at 14.079 kb on + strand at 14.164 kb on - strand, within GFF2000 at 14.199 kb on - strand, within GFF2000
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 11,548 - GFF1996 0.47 +0.5 11,716 + GFF1996 0.57 +1.2 11,770 + GFF1996 0.60 -0.6 11,771 - GFF1996 0.60 -0.1 11,771 - GFF1996 0.60 -0.2 12,100 + GFF1996 0.78 -0.1 12,100 + GFF1996 0.78 -0.0 12,136 - GFF1996 0.80 -0.9 12,170 + GFF1996 0.82 +2.0 12,171 - GFF1996 0.82 -0.4 12,214 + GFF1996 0.84 +0.0 12,214 + GFF1996 0.84 -0.3 12,215 - GFF1996 0.84 -0.4 12,217 - GFF1996 0.85 +0.2 12,255 + GFF1996 0.87 +0.6 12,255 + GFF1996 0.87 -0.1 12,469 - -0.1 12,611 + GFF1997 0.17 -0.2 12,611 + GFF1997 0.17 -0.5 12,738 - GFF1997 0.34 -0.9 12,842 + GFF1997 0.49 -0.2 12,842 + GFF1997 0.49 -0.7 12,847 + GFF1997 0.49 -0.6 12,847 + GFF1997 0.49 -0.0 12,951 - GFF1997 0.63 -0.7 13,238 + -0.6 13,251 + -0.0 13,251 + -0.6 13,252 - -0.7 13,252 - -1.0 13,305 + GFF1998 0.19 -0.3 13,306 - GFF1998 0.19 -0.6 13,307 + GFF1998 0.19 +0.1 13,308 - GFF1998 0.20 -0.4 13,594 + -0.5 13,606 - -1.6 13,737 - GFF1999 0.28 -1.3 13,767 - GFF1999 0.36 -0.3 13,874 + GFF1999 0.66 -0.7 13,939 + GFF1999 0.84 -0.1 13,993 + +0.3 14,014 + -0.2 14,079 + +0.1 14,164 - GFF2000 0.11 +0.3 14,199 - GFF2000 0.17 -0.3
Or see this region's nucleotide sequence