Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF189 and GFF190 overlap by 4 nucleotides GFF190 and GFF191 are separated by 2 nucleotides GFF191 and GFF192 are separated by 10 nucleotides GFF192 and GFF193 are separated by 105 nucleotides
GFF189 - 4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262), at 184,620 to 185,609
GFF189
GFF190 - SSU rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase (EC 2.1.1.182), at 185,606 to 186,427
GFF190
GFF191 - ApaG protein, at 186,430 to 186,807
GFF191
GFF192 - 'Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41)' transl_table=11, at 186,818 to 187,666
GFF192
GFF193 - Dihydrofolate reductase (EC 1.5.1.3), at 187,772 to 188,251
GFF193
Position (kb) 186
187 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 185.438 kb on - strand, within GFF189 at 185.867 kb on + strand, within GFF190 at 185.957 kb on - strand, within GFF190 at 185.990 kb on + strand, within GFF190 at 185.991 kb on - strand, within GFF190 at 185.992 kb on + strand, within GFF190 at 186.020 kb on - strand, within GFF190 at 186.074 kb on + strand, within GFF190 at 186.074 kb on + strand, within GFF190 at 186.105 kb on - strand, within GFF190 at 186.392 kb on + strand at 186.585 kb on + strand, within GFF191 at 186.811 kb on + strand at 186.855 kb on - strand at 186.892 kb on + strand at 186.892 kb on + strand at 186.963 kb on + strand, within GFF192 at 186.964 kb on - strand, within GFF192 at 186.969 kb on - strand, within GFF192 at 186.989 kb on - strand, within GFF192 at 187.064 kb on - strand, within GFF192 at 187.183 kb on + strand, within GFF192 at 187.336 kb on - strand, within GFF192 at 187.346 kb on - strand, within GFF192 at 187.346 kb on - strand, within GFF192 at 187.348 kb on + strand, within GFF192 at 187.400 kb on + strand, within GFF192 at 187.478 kb on + strand, within GFF192 at 187.479 kb on - strand, within GFF192 at 187.479 kb on - strand, within GFF192 at 187.605 kb on - strand at 187.605 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 185,438 - GFF189 0.83 -0.5 185,867 + GFF190 0.32 +2.2 185,957 - GFF190 0.43 -2.3 185,990 + GFF190 0.47 -0.1 185,991 - GFF190 0.47 -0.5 185,992 + GFF190 0.47 -1.8 186,020 - GFF190 0.50 +0.5 186,074 + GFF190 0.57 +0.6 186,074 + GFF190 0.57 -0.5 186,105 - GFF190 0.61 -1.3 186,392 + -0.3 186,585 + GFF191 0.41 -0.8 186,811 + -2.3 186,855 - -3.4 186,892 + -2.1 186,892 + -2.1 186,963 + GFF192 0.17 +1.0 186,964 - GFF192 0.17 -3.8 186,969 - GFF192 0.18 -3.1 186,989 - GFF192 0.20 -1.7 187,064 - GFF192 0.29 -0.4 187,183 + GFF192 0.43 -0.7 187,336 - GFF192 0.61 -1.9 187,346 - GFF192 0.62 -1.6 187,346 - GFF192 0.62 -1.9 187,348 + GFF192 0.62 -1.7 187,400 + GFF192 0.69 +0.2 187,478 + GFF192 0.78 -2.2 187,479 - GFF192 0.78 -3.0 187,479 - GFF192 0.78 -1.1 187,605 - +0.4 187,605 - +0.3
Or see this region's nucleotide sequence