Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1741 and GFF1742 overlap by 1 nucleotides GFF1742 and GFF1743 are separated by 157 nucleotides GFF1743 and GFF1744 overlap by 32 nucleotides
GFF1741 - FIG00553873: hypothetical protein, at 42,150 to 42,734
GFF1741
GFF1742 - FIG004212: hypothetical protein, at 42,734 to 43,489
GFF1742
GFF1743 - Putative LysR-family transcriptional regulator YidZ, at 43,647 to 44,606
GFF1743
GFF1744 - Putative transport protein, at 44,575 to 45,762
GFF1744
Position (kb) 43
44
45 Strain fitness (log2 ratio) -1
0
1 at 42.693 kb on - strand at 42.716 kb on + strand at 42.717 kb on - strand at 42.717 kb on - strand at 42.717 kb on - strand at 42.736 kb on - strand at 42.754 kb on - strand at 42.908 kb on + strand, within GFF1742 at 43.035 kb on - strand, within GFF1742 at 43.103 kb on + strand, within GFF1742 at 43.176 kb on - strand, within GFF1742 at 43.239 kb on + strand, within GFF1742 at 43.240 kb on - strand, within GFF1742 at 43.240 kb on - strand, within GFF1742 at 43.330 kb on - strand, within GFF1742 at 43.330 kb on - strand, within GFF1742 at 43.336 kb on + strand, within GFF1742 at 43.459 kb on + strand at 43.460 kb on - strand at 43.460 kb on - strand at 43.531 kb on - strand at 43.615 kb on + strand at 43.686 kb on + strand at 43.687 kb on - strand at 43.706 kb on + strand at 43.715 kb on + strand at 43.716 kb on - strand at 43.728 kb on + strand at 43.731 kb on + strand at 43.731 kb on + strand at 43.832 kb on - strand, within GFF1743 at 43.964 kb on + strand, within GFF1743 at 43.991 kb on + strand, within GFF1743 at 43.991 kb on + strand, within GFF1743 at 44.070 kb on + strand, within GFF1743 at 44.086 kb on - strand, within GFF1743 at 44.276 kb on + strand, within GFF1743 at 44.277 kb on - strand, within GFF1743 at 44.397 kb on - strand, within GFF1743 at 44.506 kb on - strand, within GFF1743 at 44.553 kb on + strand at 44.553 kb on + strand at 44.560 kb on - strand at 44.560 kb on - strand at 44.565 kb on + strand at 44.576 kb on + strand at 44.659 kb on + strand at 44.664 kb on + strand at 44.665 kb on - strand at 44.665 kb on - strand at 44.691 kb on + strand at 44.691 kb on + strand at 44.856 kb on + strand, within GFF1744 at 44.948 kb on - strand, within GFF1744 at 44.948 kb on - strand, within GFF1744 at 45.191 kb on - strand, within GFF1744 at 45.258 kb on + strand, within GFF1744 at 45.279 kb on + strand, within GFF1744 at 45.281 kb on + strand, within GFF1744 at 45.313 kb on - strand, within GFF1744 at 45.357 kb on + strand, within GFF1744 at 45.437 kb on + strand, within GFF1744 at 45.438 kb on - strand, within GFF1744 at 45.528 kb on + strand, within GFF1744
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 42,693 - -0.2 42,716 + -0.1 42,717 - +0.0 42,717 - +0.4 42,717 - +0.1 42,736 - -0.1 42,754 - -0.3 42,908 + GFF1742 0.23 +0.3 43,035 - GFF1742 0.40 -0.2 43,103 + GFF1742 0.49 -0.9 43,176 - GFF1742 0.58 -0.0 43,239 + GFF1742 0.67 -0.0 43,240 - GFF1742 0.67 -0.4 43,240 - GFF1742 0.67 -0.9 43,330 - GFF1742 0.79 -0.2 43,330 - GFF1742 0.79 -0.9 43,336 + GFF1742 0.80 +0.2 43,459 + -0.1 43,460 - -0.0 43,460 - -0.1 43,531 - -0.0 43,615 + -0.1 43,686 + +0.4 43,687 - +0.2 43,706 + +0.2 43,715 + -0.3 43,716 - -0.4 43,728 + +0.5 43,731 + +0.3 43,731 + +0.3 43,832 - GFF1743 0.19 +0.4 43,964 + GFF1743 0.33 +0.2 43,991 + GFF1743 0.36 -0.5 43,991 + GFF1743 0.36 -0.0 44,070 + GFF1743 0.44 +0.3 44,086 - GFF1743 0.46 +0.6 44,276 + GFF1743 0.66 +0.0 44,277 - GFF1743 0.66 -0.4 44,397 - GFF1743 0.78 +0.8 44,506 - GFF1743 0.89 +0.3 44,553 + +0.1 44,553 + +1.7 44,560 - -1.0 44,560 - +0.6 44,565 + -0.1 44,576 + -0.2 44,659 + +0.6 44,664 + -0.2 44,665 - -0.1 44,665 - +0.0 44,691 + -0.2 44,691 + -0.0 44,856 + GFF1744 0.24 +0.1 44,948 - GFF1744 0.31 -0.0 44,948 - GFF1744 0.31 -0.1 45,191 - GFF1744 0.52 -0.9 45,258 + GFF1744 0.57 +1.0 45,279 + GFF1744 0.59 -0.3 45,281 + GFF1744 0.59 -0.3 45,313 - GFF1744 0.62 +0.6 45,357 + GFF1744 0.66 +0.4 45,437 + GFF1744 0.73 +1.6 45,438 - GFF1744 0.73 -1.1 45,528 + GFF1744 0.80 -0.5
Or see this region's nucleotide sequence