Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF165 and GFF166 are separated by 2 nucleotides GFF166 and GFF167 are separated by 10 nucleotides GFF167 and GFF168 are separated by 20 nucleotides GFF168 and GFF169 are separated by 201 nucleotides
GFF165 - 3-isopropylmalate dehydrogenase (EC 1.1.1.85), at 156,923 to 158,014
GFF165
GFF166 - 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33), at 158,017 to 159,417
GFF166
GFF167 - 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33), at 159,428 to 160,033
GFF167
GFF168 - hypothetical protein, at 160,054 to 160,167
GFF168
GFF169 - Sugar-phosphate stress protein SgrT (embedded in SgrS), at 160,369 to 160,491
GFF169
Position (kb) 158
159
160 Strain fitness (log2 ratio) -1
0
1
2
3 at 157.028 kb on - strand at 157.033 kb on + strand, within GFF165 at 157.033 kb on + strand, within GFF165 at 157.034 kb on - strand, within GFF165 at 157.034 kb on - strand, within GFF165 at 157.069 kb on + strand, within GFF165 at 157.498 kb on + strand, within GFF165 at 157.515 kb on + strand, within GFF165 at 157.516 kb on - strand, within GFF165 at 157.595 kb on + strand, within GFF165 at 157.595 kb on + strand, within GFF165 at 157.595 kb on + strand, within GFF165 at 157.595 kb on + strand, within GFF165 at 157.595 kb on + strand, within GFF165 at 157.595 kb on + strand, within GFF165 at 157.596 kb on - strand, within GFF165 at 157.596 kb on - strand, within GFF165 at 157.596 kb on - strand, within GFF165 at 157.596 kb on - strand, within GFF165 at 157.597 kb on + strand, within GFF165 at 157.598 kb on - strand, within GFF165 at 157.648 kb on - strand, within GFF165 at 157.648 kb on - strand, within GFF165 at 157.854 kb on - strand, within GFF165 at 157.982 kb on - strand at 158.012 kb on + strand at 158.032 kb on + strand at 158.092 kb on + strand at 158.232 kb on + strand, within GFF166 at 158.278 kb on - strand, within GFF166 at 158.278 kb on - strand, within GFF166 at 158.309 kb on - strand, within GFF166 at 158.326 kb on + strand, within GFF166 at 158.472 kb on - strand, within GFF166 at 158.653 kb on - strand, within GFF166 at 158.740 kb on + strand, within GFF166 at 158.744 kb on + strand, within GFF166 at 158.744 kb on + strand, within GFF166 at 158.744 kb on + strand, within GFF166 at 158.745 kb on - strand, within GFF166 at 158.839 kb on - strand, within GFF166 at 158.989 kb on + strand, within GFF166 at 158.989 kb on + strand, within GFF166 at 158.990 kb on - strand, within GFF166 at 159.416 kb on - strand at 159.424 kb on + strand at 159.443 kb on - strand at 159.502 kb on + strand, within GFF167 at 159.508 kb on + strand, within GFF167 at 159.646 kb on - strand, within GFF167 at 159.721 kb on + strand, within GFF167 at 159.722 kb on - strand, within GFF167 at 159.759 kb on - strand, within GFF167 at 160.024 kb on + strand at 160.295 kb on - strand at 160.317 kb on + strand at 160.317 kb on + strand at 160.337 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 157,028 - -0.1 157,033 + GFF165 0.10 -0.4 157,033 + GFF165 0.10 +0.1 157,034 - GFF165 0.10 +0.2 157,034 - GFF165 0.10 +0.4 157,069 + GFF165 0.13 +1.9 157,498 + GFF165 0.53 +0.2 157,515 + GFF165 0.54 -0.3 157,516 - GFF165 0.54 +0.7 157,595 + GFF165 0.62 +0.7 157,595 + GFF165 0.62 +0.2 157,595 + GFF165 0.62 +0.3 157,595 + GFF165 0.62 -0.4 157,595 + GFF165 0.62 -0.4 157,595 + GFF165 0.62 -0.4 157,596 - GFF165 0.62 -1.1 157,596 - GFF165 0.62 +0.5 157,596 - GFF165 0.62 +0.8 157,596 - GFF165 0.62 +1.0 157,597 + GFF165 0.62 -0.1 157,598 - GFF165 0.62 +0.5 157,648 - GFF165 0.66 +0.2 157,648 - GFF165 0.66 -0.1 157,854 - GFF165 0.85 +0.1 157,982 - +0.6 158,012 + +0.2 158,032 + +0.7 158,092 + +0.9 158,232 + GFF166 0.15 -0.2 158,278 - GFF166 0.19 +0.2 158,278 - GFF166 0.19 -0.1 158,309 - GFF166 0.21 +0.2 158,326 + GFF166 0.22 +0.1 158,472 - GFF166 0.32 -0.2 158,653 - GFF166 0.45 +0.2 158,740 + GFF166 0.52 +0.2 158,744 + GFF166 0.52 -0.0 158,744 + GFF166 0.52 +0.0 158,744 + GFF166 0.52 +0.7 158,745 - GFF166 0.52 -0.0 158,839 - GFF166 0.59 +1.2 158,989 + GFF166 0.69 +0.4 158,989 + GFF166 0.69 +0.1 158,990 - GFF166 0.69 +1.2 159,416 - +0.3 159,424 + +0.4 159,443 - +0.1 159,502 + GFF167 0.12 +0.2 159,508 + GFF167 0.13 +0.3 159,646 - GFF167 0.36 -0.0 159,721 + GFF167 0.48 -0.1 159,722 - GFF167 0.49 -0.3 159,759 - GFF167 0.55 -0.7 160,024 + +1.6 160,295 - -0.3 160,317 + -0.5 160,317 + -1.6 160,337 + +3.3
Or see this region's nucleotide sequence