Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1650 and GFF1651 are separated by 40 nucleotides GFF1651 and GFF1652 overlap by 4 nucleotides GFF1652 and GFF1653 are separated by 235 nucleotides
GFF1650 - FIG01046209: inner membrane protein, at 35,063 to 35,458
GFF1650
GFF1651 - tRNA (uridine-5-oxyacetic acid methyl ester) 34 synthase, at 35,499 to 36,242
GFF1651
GFF1652 - tRNA (5-methoxyuridine) 34 synthase, at 36,239 to 37,210
GFF1652
GFF1653 - Cytoplasmic copper homeostasis protein CutC, at 37,446 to 38,192
GFF1653
Position (kb) 36
37
38 Strain fitness (log2 ratio) -1
0
1 at 35.288 kb on - strand, within GFF1650 at 35.424 kb on - strand at 35.424 kb on - strand at 35.424 kb on - strand at 35.426 kb on - strand at 35.498 kb on + strand at 35.498 kb on + strand at 35.499 kb on - strand at 35.614 kb on - strand, within GFF1651 at 35.644 kb on + strand, within GFF1651 at 35.679 kb on + strand, within GFF1651 at 35.727 kb on - strand, within GFF1651 at 35.765 kb on + strand, within GFF1651 at 35.811 kb on + strand, within GFF1651 at 35.822 kb on + strand, within GFF1651 at 36.066 kb on + strand, within GFF1651 at 36.479 kb on - strand, within GFF1652 at 36.539 kb on - strand, within GFF1652 at 36.641 kb on + strand, within GFF1652 at 36.946 kb on - strand, within GFF1652 at 36.992 kb on - strand, within GFF1652 at 37.051 kb on + strand, within GFF1652 at 37.231 kb on - strand at 37.234 kb on + strand at 37.234 kb on + strand at 37.234 kb on + strand at 37.234 kb on + strand at 37.255 kb on + strand at 37.302 kb on + strand at 37.302 kb on + strand at 37.310 kb on + strand at 37.418 kb on - strand at 37.503 kb on + strand at 37.509 kb on - strand at 37.509 kb on - strand at 37.519 kb on + strand at 37.710 kb on - strand, within GFF1653 at 37.886 kb on + strand, within GFF1653 at 38.031 kb on + strand, within GFF1653 at 38.042 kb on + strand, within GFF1653 at 38.046 kb on - strand, within GFF1653 at 38.065 kb on - strand, within GFF1653 at 38.185 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 35,288 - GFF1650 0.57 -0.2 35,424 - -0.7 35,424 - +0.1 35,424 - -0.2 35,426 - -1.4 35,498 + +0.7 35,498 + -0.4 35,499 - +0.6 35,614 - GFF1651 0.15 +0.4 35,644 + GFF1651 0.19 -0.4 35,679 + GFF1651 0.24 +0.7 35,727 - GFF1651 0.31 -0.1 35,765 + GFF1651 0.36 +0.1 35,811 + GFF1651 0.42 -0.4 35,822 + GFF1651 0.43 -0.2 36,066 + GFF1651 0.76 -1.6 36,479 - GFF1652 0.25 -0.6 36,539 - GFF1652 0.31 -0.6 36,641 + GFF1652 0.41 +1.0 36,946 - GFF1652 0.73 -0.8 36,992 - GFF1652 0.77 -0.6 37,051 + GFF1652 0.84 -0.7 37,231 - -0.7 37,234 + +0.2 37,234 + +1.2 37,234 + +0.1 37,234 + +0.3 37,255 + +0.1 37,302 + +0.0 37,302 + -0.1 37,310 + +0.6 37,418 - -1.4 37,503 + -0.3 37,509 - -1.4 37,509 - +0.2 37,519 + +0.0 37,710 - GFF1653 0.35 +0.0 37,886 + GFF1653 0.59 +0.2 38,031 + GFF1653 0.78 -0.1 38,042 + GFF1653 0.80 +0.2 38,046 - GFF1653 0.80 +0.1 38,065 - GFF1653 0.83 +0.7 38,185 - +0.1
Or see this region's nucleotide sequence