Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1597 and GFF1598 are separated by 76 nucleotides GFF1598 and GFF1599 overlap by 4 nucleotides GFF1599 and GFF1600 are separated by 82 nucleotides GFF1600 and GFF1601 are separated by 126 nucleotides GFF1601 and GFF1602 are separated by 193 nucleotides
GFF1597 - Glutamate--cysteine ligase (EC 6.3.2.2), at 55,117 to 56,673
GFF1597
GFF1598 - Putative membrane protein, at 56,750 to 57,175
GFF1598
GFF1599 - Putative phosphatase YqaB, at 57,172 to 57,738
GFF1599
GFF1600 - HAD-superfamily hydrolase, subfamily IA, at 57,821 to 57,982
GFF1600
GFF1601 - tRNA-Arg-ACG, at 58,109 to 58,185
GFF1601
GFF1602 - tRNA-Arg-ACG, at 58,379 to 58,455
GFF1602
Position (kb) 57
58 Strain fitness (log2 ratio) -1
0
1 at 56.227 kb on + strand, within GFF1597 at 56.333 kb on - strand, within GFF1597 at 56.355 kb on - strand, within GFF1597 at 56.355 kb on - strand, within GFF1597 at 56.364 kb on + strand, within GFF1597 at 56.365 kb on - strand, within GFF1597 at 56.407 kb on - strand, within GFF1597 at 56.427 kb on + strand, within GFF1597 at 56.428 kb on - strand, within GFF1597 at 56.440 kb on - strand, within GFF1597 at 56.440 kb on - strand, within GFF1597 at 56.440 kb on - strand, within GFF1597 at 56.530 kb on + strand at 56.706 kb on - strand at 56.711 kb on + strand at 56.712 kb on - strand at 56.718 kb on - strand at 56.730 kb on - strand at 56.748 kb on + strand at 56.909 kb on + strand, within GFF1598 at 56.910 kb on - strand, within GFF1598 at 56.910 kb on - strand, within GFF1598 at 57.004 kb on + strand, within GFF1598 at 57.005 kb on - strand, within GFF1598 at 57.005 kb on - strand, within GFF1598 at 57.005 kb on - strand, within GFF1598 at 57.023 kb on - strand, within GFF1598 at 57.047 kb on + strand, within GFF1598 at 57.082 kb on + strand, within GFF1598 at 57.083 kb on - strand, within GFF1598 at 57.178 kb on + strand at 57.556 kb on - strand, within GFF1599 at 57.638 kb on + strand, within GFF1599 at 57.706 kb on - strand at 57.726 kb on - strand at 57.735 kb on - strand at 57.803 kb on - strand at 57.816 kb on + strand at 57.817 kb on - strand at 57.817 kb on - strand at 57.854 kb on + strand, within GFF1600 at 57.940 kb on + strand, within GFF1600 at 58.063 kb on + strand at 58.065 kb on + strand at 58.070 kb on - strand at 58.237 kb on - strand at 58.344 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 56,227 + GFF1597 0.71 -0.1 56,333 - GFF1597 0.78 +0.7 56,355 - GFF1597 0.80 +0.4 56,355 - GFF1597 0.80 -0.3 56,364 + GFF1597 0.80 -1.1 56,365 - GFF1597 0.80 +0.5 56,407 - GFF1597 0.83 -0.2 56,427 + GFF1597 0.84 -0.5 56,428 - GFF1597 0.84 +0.7 56,440 - GFF1597 0.85 +0.5 56,440 - GFF1597 0.85 +0.6 56,440 - GFF1597 0.85 -0.5 56,530 + -0.2 56,706 - +0.2 56,711 + +0.3 56,712 - +0.5 56,718 - +0.1 56,730 - +0.1 56,748 + -0.4 56,909 + GFF1598 0.37 -1.7 56,910 - GFF1598 0.38 -0.1 56,910 - GFF1598 0.38 -0.6 57,004 + GFF1598 0.60 +0.3 57,005 - GFF1598 0.60 -0.3 57,005 - GFF1598 0.60 +0.0 57,005 - GFF1598 0.60 -0.7 57,023 - GFF1598 0.64 -0.0 57,047 + GFF1598 0.70 -0.5 57,082 + GFF1598 0.78 +1.3 57,083 - GFF1598 0.78 +0.3 57,178 + +0.1 57,556 - GFF1599 0.68 +0.5 57,638 + GFF1599 0.82 +0.2 57,706 - +0.1 57,726 - -0.0 57,735 - +1.1 57,803 - +0.0 57,816 + -0.1 57,817 - -0.2 57,817 - -0.3 57,854 + GFF1600 0.20 +0.1 57,940 + GFF1600 0.73 -0.5 58,063 + +0.3 58,065 + +0.3 58,070 - -1.2 58,237 - -0.2 58,344 + +0.9
Or see this region's nucleotide sequence