Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1455 and GFF1456 overlap by 4 nucleotides GFF1456 and GFF1457 overlap by 8 nucleotides GFF1457 and GFF1458 overlap by 4 nucleotides GFF1458 and GFF1459 are separated by 139 nucleotides GFF1459 and GFF1460 are separated by 76 nucleotides
GFF1455 - NrfC protein, at 118,346 to 119,017
GFF1455
GFF1456 - NrfD protein, at 119,014 to 119,970
GFF1456
GFF1457 - Cytochrome c heme lyase subunit CcmF, at 119,963 to 122,185
GFF1457
GFF1458 - Cytochrome c-type heme lyase subunit nrfG, nitrite reductase complex assembly, at 122,182 to 122,802
GFF1458
GFF1459 - hypothetical protein, at 122,942 to 123,070
GFF1459
GFF1460 - Glutamate-aspartate carrier protein, at 123,147 to 124,457
GFF1460
Position (kb)
119
120
121
122
123 Strain fitness (log2 ratio)
-1
0
1 at 118.994 kb on - strand at 118.997 kb on + strand at 118.998 kb on - strand at 119.075 kb on - strand at 119.164 kb on - strand, within GFF1456 at 119.164 kb on - strand, within GFF1456 at 119.275 kb on + strand, within GFF1456 at 119.323 kb on + strand, within GFF1456 at 119.330 kb on + strand, within GFF1456 at 119.330 kb on + strand, within GFF1456 at 119.331 kb on - strand, within GFF1456 at 119.466 kb on - strand, within GFF1456 at 119.497 kb on + strand, within GFF1456 at 119.545 kb on - strand, within GFF1456 at 119.631 kb on + strand, within GFF1456 at 119.632 kb on - strand, within GFF1456 at 119.632 kb on - strand, within GFF1456 at 119.663 kb on + strand, within GFF1456 at 119.687 kb on + strand, within GFF1456 at 119.687 kb on + strand, within GFF1456 at 119.688 kb on - strand, within GFF1456 at 119.688 kb on - strand, within GFF1456 at 119.866 kb on - strand, within GFF1456 at 120.155 kb on - strand at 120.377 kb on - strand, within GFF1457 at 120.397 kb on + strand, within GFF1457 at 120.398 kb on - strand, within GFF1457 at 120.493 kb on - strand, within GFF1457 at 120.669 kb on - strand, within GFF1457 at 120.767 kb on + strand, within GFF1457 at 120.767 kb on + strand, within GFF1457 at 120.768 kb on - strand, within GFF1457 at 120.925 kb on + strand, within GFF1457 at 120.966 kb on - strand, within GFF1457 at 121.104 kb on - strand, within GFF1457 at 121.104 kb on - strand, within GFF1457 at 121.189 kb on - strand, within GFF1457 at 121.269 kb on + strand, within GFF1457 at 121.270 kb on - strand, within GFF1457 at 121.270 kb on - strand, within GFF1457 at 121.270 kb on - strand, within GFF1457 at 121.364 kb on + strand, within GFF1457 at 121.365 kb on - strand, within GFF1457 at 121.410 kb on - strand, within GFF1457 at 121.410 kb on - strand, within GFF1457 at 121.410 kb on - strand, within GFF1457 at 121.410 kb on - strand, within GFF1457 at 121.539 kb on + strand, within GFF1457 at 121.599 kb on + strand, within GFF1457 at 121.601 kb on + strand, within GFF1457 at 121.601 kb on + strand, within GFF1457 at 121.601 kb on + strand, within GFF1457 at 121.602 kb on - strand, within GFF1457 at 121.602 kb on - strand, within GFF1457 at 121.804 kb on - strand, within GFF1457 at 122.003 kb on - strand at 122.229 kb on - strand at 122.452 kb on + strand, within GFF1458 at 122.452 kb on + strand, within GFF1458 at 122.452 kb on + strand, within GFF1458 at 122.577 kb on + strand, within GFF1458 at 122.577 kb on + strand, within GFF1458 at 122.577 kb on + strand, within GFF1458 at 122.578 kb on - strand, within GFF1458 at 122.578 kb on - strand, within GFF1458 at 122.578 kb on - strand, within GFF1458 at 122.607 kb on + strand, within GFF1458 at 122.607 kb on + strand, within GFF1458 at 122.607 kb on + strand, within GFF1458 at 122.608 kb on - strand, within GFF1458 at 123.028 kb on - strand, within GFF1459 at 123.035 kb on - strand, within GFF1459 at 123.088 kb on + strand at 123.089 kb on - strand at 123.092 kb on + strand at 123.093 kb on - strand at 123.093 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 118,994 - -0.1 118,997 + -0.4 118,998 - -0.5 119,075 - -0.3 119,164 - GFF1456 0.16 +0.1 119,164 - GFF1456 0.16 -0.3 119,275 + GFF1456 0.27 -0.1 119,323 + GFF1456 0.32 +0.6 119,330 + GFF1456 0.33 -0.3 119,330 + GFF1456 0.33 +1.6 119,331 - GFF1456 0.33 -0.9 119,466 - GFF1456 0.47 -0.1 119,497 + GFF1456 0.50 +0.3 119,545 - GFF1456 0.55 -0.0 119,631 + GFF1456 0.64 -0.3 119,632 - GFF1456 0.65 +0.1 119,632 - GFF1456 0.65 +1.1 119,663 + GFF1456 0.68 -0.1 119,687 + GFF1456 0.70 +0.3 119,687 + GFF1456 0.70 +0.3 119,688 - GFF1456 0.70 -0.2 119,688 - GFF1456 0.70 -0.6 119,866 - GFF1456 0.89 -0.0 120,155 - -0.3 120,377 - GFF1457 0.19 -0.3 120,397 + GFF1457 0.20 +0.2 120,398 - GFF1457 0.20 -0.0 120,493 - GFF1457 0.24 +1.6 120,669 - GFF1457 0.32 +0.3 120,767 + GFF1457 0.36 +0.5 120,767 + GFF1457 0.36 -0.9 120,768 - GFF1457 0.36 -0.1 120,925 + GFF1457 0.43 -1.3 120,966 - GFF1457 0.45 -0.4 121,104 - GFF1457 0.51 +0.1 121,104 - GFF1457 0.51 -0.4 121,189 - GFF1457 0.55 +0.5 121,269 + GFF1457 0.59 +1.2 121,270 - GFF1457 0.59 +0.3 121,270 - GFF1457 0.59 -0.0 121,270 - GFF1457 0.59 +0.6 121,364 + GFF1457 0.63 -1.0 121,365 - GFF1457 0.63 +0.2 121,410 - GFF1457 0.65 +0.5 121,410 - GFF1457 0.65 +0.1 121,410 - GFF1457 0.65 +0.6 121,410 - GFF1457 0.65 +0.1 121,539 + GFF1457 0.71 -0.4 121,599 + GFF1457 0.74 +0.2 121,601 + GFF1457 0.74 +0.2 121,601 + GFF1457 0.74 +0.1 121,601 + GFF1457 0.74 +0.3 121,602 - GFF1457 0.74 +0.1 121,602 - GFF1457 0.74 +0.2 121,804 - GFF1457 0.83 +0.1 122,003 - -0.2 122,229 - +0.1 122,452 + GFF1458 0.43 +0.0 122,452 + GFF1458 0.43 -0.2 122,452 + GFF1458 0.43 +0.3 122,577 + GFF1458 0.64 +0.2 122,577 + GFF1458 0.64 +0.5 122,577 + GFF1458 0.64 +0.2 122,578 - GFF1458 0.64 +0.2 122,578 - GFF1458 0.64 +0.3 122,578 - GFF1458 0.64 +0.3 122,607 + GFF1458 0.68 -0.3 122,607 + GFF1458 0.68 +0.2 122,607 + GFF1458 0.68 +1.3 122,608 - GFF1458 0.69 +0.0 123,028 - GFF1459 0.67 -0.9 123,035 - GFF1459 0.72 -0.1 123,088 + +0.8 123,089 - -0.5 123,092 + -0.1 123,093 - +1.1 123,093 - +0.3
Or see this region's nucleotide sequence