Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1453 and GFF1454 are separated by 111 nucleotides GFF1454 and GFF1455 overlap by 4 nucleotides GFF1455 and GFF1456 overlap by 4 nucleotides
GFF1453 - Cytochrome c552 precursor (EC 1.7.2.2), at 116,235 to 117,671
GFF1453
GFF1454 - Cytochrome c-type protein NrfB precursor, at 117,783 to 118,349
GFF1454
GFF1455 - NrfC protein, at 118,346 to 119,017
GFF1455
GFF1456 - NrfD protein, at 119,014 to 119,970
GFF1456
Position (kb)
117
118
119 Strain fitness (log2 ratio)
-1
0
1 at 116.881 kb on - strand, within GFF1453 at 116.909 kb on + strand, within GFF1453 at 116.955 kb on + strand, within GFF1453 at 116.966 kb on + strand, within GFF1453 at 117.137 kb on + strand, within GFF1453 at 117.277 kb on + strand, within GFF1453 at 117.278 kb on - strand, within GFF1453 at 117.278 kb on - strand, within GFF1453 at 117.408 kb on - strand, within GFF1453 at 117.408 kb on - strand, within GFF1453 at 117.474 kb on - strand, within GFF1453 at 117.575 kb on + strand at 117.576 kb on - strand at 117.737 kb on - strand at 117.766 kb on + strand at 117.767 kb on - strand at 117.771 kb on - strand at 117.771 kb on - strand at 117.782 kb on + strand at 117.783 kb on - strand at 117.803 kb on - strand at 117.998 kb on + strand, within GFF1454 at 118.093 kb on - strand, within GFF1454 at 118.093 kb on - strand, within GFF1454 at 118.095 kb on - strand, within GFF1454 at 118.100 kb on - strand, within GFF1454 at 118.121 kb on + strand, within GFF1454 at 118.122 kb on - strand, within GFF1454 at 118.122 kb on - strand, within GFF1454 at 118.441 kb on + strand, within GFF1455 at 118.559 kb on - strand, within GFF1455 at 118.769 kb on - strand, within GFF1455 at 118.795 kb on + strand, within GFF1455 at 118.940 kb on + strand, within GFF1455 at 118.994 kb on - strand at 118.997 kb on + strand at 118.998 kb on - strand at 119.075 kb on - strand at 119.164 kb on - strand, within GFF1456 at 119.164 kb on - strand, within GFF1456 at 119.275 kb on + strand, within GFF1456 at 119.323 kb on + strand, within GFF1456 at 119.330 kb on + strand, within GFF1456 at 119.330 kb on + strand, within GFF1456 at 119.331 kb on - strand, within GFF1456
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 116,881 - GFF1453 0.45 +0.5 116,909 + GFF1453 0.47 -0.4 116,955 + GFF1453 0.50 +1.1 116,966 + GFF1453 0.51 -1.0 117,137 + GFF1453 0.63 +0.4 117,277 + GFF1453 0.73 +0.1 117,278 - GFF1453 0.73 -0.4 117,278 - GFF1453 0.73 -0.1 117,408 - GFF1453 0.82 -0.6 117,408 - GFF1453 0.82 -0.0 117,474 - GFF1453 0.86 -0.2 117,575 + +0.2 117,576 - +0.5 117,737 - +1.1 117,766 + +0.6 117,767 - -0.9 117,771 - -0.7 117,771 - -0.0 117,782 + +1.0 117,783 - -0.1 117,803 - -0.1 117,998 + GFF1454 0.38 +0.4 118,093 - GFF1454 0.55 -0.4 118,093 - GFF1454 0.55 +0.4 118,095 - GFF1454 0.55 +0.7 118,100 - GFF1454 0.56 +0.2 118,121 + GFF1454 0.60 +0.0 118,122 - GFF1454 0.60 +0.5 118,122 - GFF1454 0.60 +0.3 118,441 + GFF1455 0.14 -0.5 118,559 - GFF1455 0.32 -0.0 118,769 - GFF1455 0.63 -0.6 118,795 + GFF1455 0.67 +0.1 118,940 + GFF1455 0.88 -0.1 118,994 - -0.1 118,997 + -0.4 118,998 - -0.5 119,075 - -0.3 119,164 - GFF1456 0.16 +0.1 119,164 - GFF1456 0.16 -0.3 119,275 + GFF1456 0.27 -0.1 119,323 + GFF1456 0.32 +0.6 119,330 + GFF1456 0.33 -0.3 119,330 + GFF1456 0.33 +1.6 119,331 - GFF1456 0.33 -0.9
Or see this region's nucleotide sequence