Experiment: Acanthamoeba sp., muxed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1361 and GFF1362 are separated by 46 nucleotides GFF1362 and GFF1363 overlap by 4 nucleotides GFF1363 and GFF1364 are separated by 97 nucleotides
GFF1361 - Nucleoprotein/polynucleotide-associated enzyme, at 15,021 to 15,560
GFF1361
GFF1362 - Ribosomal large subunit pseudouridine synthase F (EC 4.2.1.70), at 15,607 to 16,476
GFF1362
GFF1363 - hypothetical protein, at 16,473 to 16,745
GFF1363
GFF1364 - Sodium-dependent transporter, at 16,843 to 17,784
GFF1364
Position (kb)
16
17 Strain fitness (log2 ratio)
-1
0
1 at 15.559 kb on - strand at 15.559 kb on - strand at 15.583 kb on - strand at 15.606 kb on + strand at 15.640 kb on + strand at 15.640 kb on + strand at 15.640 kb on + strand at 15.642 kb on + strand at 15.806 kb on + strand, within GFF1362 at 15.806 kb on + strand, within GFF1362 at 15.948 kb on - strand, within GFF1362 at 16.107 kb on + strand, within GFF1362 at 16.180 kb on - strand, within GFF1362 at 16.537 kb on + strand, within GFF1363 at 16.601 kb on + strand, within GFF1363 at 16.610 kb on - strand, within GFF1363 at 17.107 kb on - strand, within GFF1364 at 17.151 kb on + strand, within GFF1364 at 17.152 kb on - strand, within GFF1364 at 17.455 kb on + strand, within GFF1364 at 17.456 kb on - strand, within GFF1364 at 17.563 kb on + strand, within GFF1364 at 17.661 kb on + strand, within GFF1364 at 17.662 kb on - strand, within GFF1364 at 17.720 kb on + strand at 17.723 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Acanthamoeba sp., muxed culture-1 remove 15,559 - +0.1 15,559 - +1.6 15,583 - +0.1 15,606 + -0.6 15,640 + -0.3 15,640 + -1.4 15,640 + +1.0 15,642 + +0.6 15,806 + GFF1362 0.23 +0.1 15,806 + GFF1362 0.23 -0.2 15,948 - GFF1362 0.39 +0.4 16,107 + GFF1362 0.57 -0.2 16,180 - GFF1362 0.66 +0.3 16,537 + GFF1363 0.23 +0.9 16,601 + GFF1363 0.47 +0.9 16,610 - GFF1363 0.50 +0.9 17,107 - GFF1364 0.28 -0.5 17,151 + GFF1364 0.33 -0.1 17,152 - GFF1364 0.33 +0.3 17,455 + GFF1364 0.65 -0.1 17,456 - GFF1364 0.65 -0.1 17,563 + GFF1364 0.76 -0.0 17,661 + GFF1364 0.87 +0.6 17,662 - GFF1364 0.87 +0.0 17,720 + -0.3 17,723 + -0.1
Or see this region's nucleotide sequence