Experiment: LB Aerobic with Doxycycline hyclate 0.00000625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mltF and tadA overlap by 4 nucleotides tadA and yfhB are separated by 57 nucleotides yfhB and yfhH are separated by 208 nucleotides
b2558: mltF - predicted periplasmic binding protein/transglycosylase (RefSeq), at 2,693,823 to 2,695,379
mltF
b2559: tadA - tRNA-specific adenosine deaminase (RefSeq), at 2,695,376 to 2,695,879
tadA
b2560: yfhB - orf, hypothetical protein (VIMSS), at 2,695,937 to 2,696,572
yfhB
b2561: yfhH - orf, hypothetical protein (VIMSS), at 2,696,781 to 2,697,629
yfhH
Position (kb)
2695
2696
2697 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2694.969 kb on + strand, within mltF at 2694.971 kb on + strand, within mltF at 2694.976 kb on + strand, within mltF at 2695.086 kb on + strand, within mltF at 2695.109 kb on + strand, within mltF at 2695.125 kb on + strand, within mltF at 2695.138 kb on - strand, within mltF at 2695.203 kb on - strand, within mltF at 2695.203 kb on - strand, within mltF at 2695.252 kb on + strand at 2695.252 kb on + strand at 2695.256 kb on - strand at 2695.260 kb on - strand at 2695.328 kb on + strand at 2695.365 kb on - strand at 2695.945 kb on - strand at 2695.945 kb on - strand at 2695.966 kb on + strand at 2695.966 kb on + strand at 2695.968 kb on + strand at 2695.968 kb on + strand at 2696.004 kb on + strand, within yfhB at 2696.004 kb on + strand, within yfhB at 2696.014 kb on + strand, within yfhB at 2696.014 kb on + strand, within yfhB at 2696.024 kb on + strand, within yfhB at 2696.044 kb on + strand, within yfhB at 2696.044 kb on - strand, within yfhB at 2696.050 kb on - strand, within yfhB at 2696.077 kb on - strand, within yfhB at 2696.077 kb on - strand, within yfhB at 2696.089 kb on + strand, within yfhB at 2696.089 kb on + strand, within yfhB at 2696.164 kb on - strand, within yfhB at 2696.477 kb on + strand, within yfhB at 2696.479 kb on + strand, within yfhB at 2696.479 kb on + strand, within yfhB at 2696.526 kb on + strand at 2696.543 kb on - strand at 2696.543 kb on - strand at 2696.571 kb on + strand at 2696.579 kb on - strand at 2696.582 kb on - strand at 2696.673 kb on - strand at 2696.673 kb on - strand at 2696.739 kb on + strand at 2696.828 kb on + strand at 2696.830 kb on + strand at 2696.916 kb on - strand, within yfhH at 2697.042 kb on - strand, within yfhH at 2697.102 kb on + strand, within yfhH at 2697.162 kb on + strand, within yfhH at 2697.181 kb on + strand, within yfhH at 2697.290 kb on + strand, within yfhH at 2697.290 kb on + strand, within yfhH at 2697.290 kb on + strand, within yfhH at 2697.320 kb on - strand, within yfhH at 2697.326 kb on + strand, within yfhH at 2697.326 kb on + strand, within yfhH at 2697.334 kb on - strand, within yfhH at 2697.355 kb on + strand, within yfhH at 2697.446 kb on + strand, within yfhH at 2697.472 kb on - strand, within yfhH at 2697.514 kb on + strand, within yfhH
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Doxycycline hyclate 0.00000625 mM remove 2,694,969 + mltF b2558 0.74 -0.6 2,694,971 + mltF b2558 0.74 -1.1 2,694,976 + mltF b2558 0.74 -0.6 2,695,086 + mltF b2558 0.81 -0.5 2,695,109 + mltF b2558 0.83 +0.2 2,695,125 + mltF b2558 0.84 -1.4 2,695,138 - mltF b2558 0.84 -1.6 2,695,203 - mltF b2558 0.89 -0.2 2,695,203 - mltF b2558 0.89 -0.1 2,695,252 + +0.9 2,695,252 + +1.2 2,695,256 - -0.5 2,695,260 - +1.3 2,695,328 + +0.4 2,695,365 - -0.1 2,695,945 - +1.5 2,695,945 - -1.2 2,695,966 + +0.9 2,695,966 + +0.7 2,695,968 + -0.7 2,695,968 + +1.9 2,696,004 + yfhB b2560 0.11 +0.9 2,696,004 + yfhB b2560 0.11 +0.4 2,696,014 + yfhB b2560 0.12 +0.1 2,696,014 + yfhB b2560 0.12 +0.1 2,696,024 + yfhB b2560 0.14 -0.8 2,696,044 + yfhB b2560 0.17 +0.5 2,696,044 - yfhB b2560 0.17 -0.3 2,696,050 - yfhB b2560 0.18 -0.2 2,696,077 - yfhB b2560 0.22 +1.5 2,696,077 - yfhB b2560 0.22 -1.4 2,696,089 + yfhB b2560 0.24 -1.1 2,696,089 + yfhB b2560 0.24 +0.1 2,696,164 - yfhB b2560 0.36 -0.2 2,696,477 + yfhB b2560 0.85 -0.2 2,696,479 + yfhB b2560 0.85 +1.2 2,696,479 + yfhB b2560 0.85 -2.1 2,696,526 + +0.9 2,696,543 - +0.5 2,696,543 - -1.1 2,696,571 + +0.8 2,696,579 - -0.0 2,696,582 - -0.8 2,696,673 - -0.3 2,696,673 - -0.4 2,696,739 + +0.8 2,696,828 + -0.4 2,696,830 + -0.0 2,696,916 - yfhH b2561 0.16 +0.4 2,697,042 - yfhH b2561 0.31 -1.7 2,697,102 + yfhH b2561 0.38 -0.0 2,697,162 + yfhH b2561 0.45 +1.3 2,697,181 + yfhH b2561 0.47 -0.5 2,697,290 + yfhH b2561 0.60 +0.2 2,697,290 + yfhH b2561 0.60 +2.9 2,697,290 + yfhH b2561 0.60 -0.1 2,697,320 - yfhH b2561 0.63 +0.2 2,697,326 + yfhH b2561 0.64 -0.4 2,697,326 + yfhH b2561 0.64 -0.6 2,697,334 - yfhH b2561 0.65 +0.3 2,697,355 + yfhH b2561 0.68 +0.6 2,697,446 + yfhH b2561 0.78 -0.9 2,697,472 - yfhH b2561 0.81 +0.4 2,697,514 + yfhH b2561 0.86 -1.7
Or see this region's nucleotide sequence