Experiment: LB Aerobic with Doxycycline hyclate 0.00000625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisF and hisI overlap by 7 nucleotides hisI and wzzB are separated by 95 nucleotides wzzB and ugd are separated by 145 nucleotides
b2025: hisF - imidazole glycerol phosphate synthase subunit HisF (NCBI), at 2,093,868 to 2,094,644
hisF
b2026: hisI - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein (NCBI), at 2,094,638 to 2,095,249
hisI
b2027: wzzB - regulator of length of O-antigen component of lipopolysaccharide chains (VIMSS), at 2,095,345 to 2,096,325
wzzB
b2028: ugd - UDP-glucose 6-dehydrogenase (NCBI), at 2,096,471 to 2,097,637
ugd
Position (kb)
2095
2096
2097 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2094.596 kb on + strand at 2094.596 kb on + strand at 2094.647 kb on + strand at 2094.712 kb on - strand, within hisI at 2094.712 kb on - strand, within hisI at 2094.716 kb on + strand, within hisI at 2094.869 kb on + strand, within hisI at 2094.907 kb on - strand, within hisI at 2094.907 kb on - strand, within hisI at 2095.032 kb on + strand, within hisI at 2095.033 kb on + strand, within hisI at 2095.033 kb on + strand, within hisI at 2095.124 kb on + strand, within hisI at 2095.167 kb on + strand, within hisI at 2095.202 kb on - strand at 2095.231 kb on + strand at 2095.386 kb on - strand at 2095.490 kb on + strand, within wzzB at 2095.494 kb on + strand, within wzzB at 2095.586 kb on - strand, within wzzB at 2095.586 kb on - strand, within wzzB at 2095.606 kb on - strand, within wzzB at 2095.719 kb on - strand, within wzzB at 2095.719 kb on - strand, within wzzB at 2095.729 kb on - strand, within wzzB at 2095.819 kb on + strand, within wzzB at 2095.819 kb on + strand, within wzzB at 2095.900 kb on + strand, within wzzB at 2095.900 kb on + strand, within wzzB at 2096.075 kb on + strand, within wzzB at 2096.075 kb on + strand, within wzzB at 2096.102 kb on + strand, within wzzB at 2096.163 kb on - strand, within wzzB at 2096.458 kb on + strand at 2096.458 kb on + strand at 2096.467 kb on + strand at 2096.480 kb on + strand at 2096.480 kb on + strand at 2096.486 kb on - strand at 2096.496 kb on + strand at 2096.498 kb on + strand at 2096.498 kb on + strand at 2096.519 kb on + strand at 2096.633 kb on + strand, within ugd at 2096.634 kb on + strand, within ugd at 2096.634 kb on + strand, within ugd at 2096.638 kb on - strand, within ugd at 2096.638 kb on - strand, within ugd at 2096.641 kb on - strand, within ugd at 2096.641 kb on - strand, within ugd at 2096.669 kb on - strand, within ugd at 2096.683 kb on + strand, within ugd at 2096.746 kb on + strand, within ugd at 2096.846 kb on - strand, within ugd at 2096.846 kb on - strand, within ugd at 2096.850 kb on + strand, within ugd at 2096.858 kb on - strand, within ugd at 2096.861 kb on - strand, within ugd at 2096.862 kb on - strand, within ugd at 2096.862 kb on - strand, within ugd at 2096.880 kb on + strand, within ugd at 2096.909 kb on + strand, within ugd at 2096.909 kb on + strand, within ugd at 2096.917 kb on - strand, within ugd at 2096.917 kb on - strand, within ugd at 2096.918 kb on - strand, within ugd at 2096.918 kb on - strand, within ugd at 2096.922 kb on + strand, within ugd at 2096.922 kb on + strand, within ugd at 2096.998 kb on - strand, within ugd at 2097.030 kb on - strand, within ugd at 2097.035 kb on - strand, within ugd at 2097.058 kb on - strand, within ugd at 2097.074 kb on + strand, within ugd at 2097.111 kb on + strand, within ugd at 2097.111 kb on + strand, within ugd at 2097.111 kb on + strand, within ugd at 2097.113 kb on + strand, within ugd at 2097.119 kb on - strand, within ugd at 2097.121 kb on + strand, within ugd at 2097.121 kb on - strand, within ugd at 2097.129 kb on - strand, within ugd at 2097.141 kb on + strand, within ugd at 2097.166 kb on - strand, within ugd at 2097.166 kb on - strand, within ugd at 2097.195 kb on + strand, within ugd at 2097.195 kb on + strand, within ugd at 2097.271 kb on + strand, within ugd at 2097.278 kb on + strand, within ugd at 2097.297 kb on + strand, within ugd
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Doxycycline hyclate 0.00000625 mM remove 2,094,596 + +0.8 2,094,596 + -0.2 2,094,647 + -0.5 2,094,712 - hisI b2026 0.12 +0.4 2,094,712 - hisI b2026 0.12 -1.2 2,094,716 + hisI b2026 0.13 -1.2 2,094,869 + hisI b2026 0.38 -2.1 2,094,907 - hisI b2026 0.44 +0.3 2,094,907 - hisI b2026 0.44 +0.8 2,095,032 + hisI b2026 0.64 -0.3 2,095,033 + hisI b2026 0.65 +0.2 2,095,033 + hisI b2026 0.65 -1.4 2,095,124 + hisI b2026 0.79 +0.1 2,095,167 + hisI b2026 0.86 -0.3 2,095,202 - -0.9 2,095,231 + -0.8 2,095,386 - +0.2 2,095,490 + wzzB b2027 0.15 -0.8 2,095,494 + wzzB b2027 0.15 +0.8 2,095,586 - wzzB b2027 0.25 -0.6 2,095,586 - wzzB b2027 0.25 -0.4 2,095,606 - wzzB b2027 0.27 -2.6 2,095,719 - wzzB b2027 0.38 -2.1 2,095,719 - wzzB b2027 0.38 -0.8 2,095,729 - wzzB b2027 0.39 +0.1 2,095,819 + wzzB b2027 0.48 -0.8 2,095,819 + wzzB b2027 0.48 -2.0 2,095,900 + wzzB b2027 0.57 -0.2 2,095,900 + wzzB b2027 0.57 +0.8 2,096,075 + wzzB b2027 0.74 -1.5 2,096,075 + wzzB b2027 0.74 +0.8 2,096,102 + wzzB b2027 0.77 -1.0 2,096,163 - wzzB b2027 0.83 +0.2 2,096,458 + -1.2 2,096,458 + -0.2 2,096,467 + +0.8 2,096,480 + -0.2 2,096,480 + -1.5 2,096,486 - +0.7 2,096,496 + +0.8 2,096,498 + -0.2 2,096,498 + -1.5 2,096,519 + -0.4 2,096,633 + ugd b2028 0.14 -0.5 2,096,634 + ugd b2028 0.14 -0.2 2,096,634 + ugd b2028 0.14 +2.4 2,096,638 - ugd b2028 0.14 -0.3 2,096,638 - ugd b2028 0.14 +1.4 2,096,641 - ugd b2028 0.15 -0.8 2,096,641 - ugd b2028 0.15 +1.2 2,096,669 - ugd b2028 0.17 -0.5 2,096,683 + ugd b2028 0.18 -0.8 2,096,746 + ugd b2028 0.24 -0.8 2,096,846 - ugd b2028 0.32 +1.1 2,096,846 - ugd b2028 0.32 +0.2 2,096,850 + ugd b2028 0.32 -0.9 2,096,858 - ugd b2028 0.33 +0.5 2,096,861 - ugd b2028 0.33 -0.4 2,096,862 - ugd b2028 0.34 -0.3 2,096,862 - ugd b2028 0.34 -0.3 2,096,880 + ugd b2028 0.35 -0.3 2,096,909 + ugd b2028 0.38 -0.2 2,096,909 + ugd b2028 0.38 +0.3 2,096,917 - ugd b2028 0.38 +0.2 2,096,917 - ugd b2028 0.38 -0.2 2,096,918 - ugd b2028 0.38 +0.8 2,096,918 - ugd b2028 0.38 +2.8 2,096,922 + ugd b2028 0.39 -0.2 2,096,922 + ugd b2028 0.39 -1.0 2,096,998 - ugd b2028 0.45 -0.2 2,097,030 - ugd b2028 0.48 +0.8 2,097,035 - ugd b2028 0.48 -1.6 2,097,058 - ugd b2028 0.50 -2.3 2,097,074 + ugd b2028 0.52 -0.2 2,097,111 + ugd b2028 0.55 +0.8 2,097,111 + ugd b2028 0.55 -0.5 2,097,111 + ugd b2028 0.55 -0.4 2,097,113 + ugd b2028 0.55 +1.5 2,097,119 - ugd b2028 0.56 +0.8 2,097,121 + ugd b2028 0.56 -0.8 2,097,121 - ugd b2028 0.56 -0.2 2,097,129 - ugd b2028 0.56 +1.8 2,097,141 + ugd b2028 0.57 -0.8 2,097,166 - ugd b2028 0.60 +0.8 2,097,166 - ugd b2028 0.60 +0.8 2,097,195 + ugd b2028 0.62 -0.8 2,097,195 + ugd b2028 0.62 -1.8 2,097,271 + ugd b2028 0.69 -0.5 2,097,278 + ugd b2028 0.69 +0.4 2,097,297 + ugd b2028 0.71 -0.1
Or see this region's nucleotide sequence