Strain Fitness in Escherichia coli BW25113 around b1119

Experiment: LB Aerobic with Doxycycline hyclate 0.00000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntlolE and nagK are separated by 28 nucleotidesnagK and cobB are separated by 126 nucleotidescobB and ycfZ are separated by 119 nucleotides b1118: lolE - outer membrane-specific lipoprotein transporter subunit (NCBI), at 1,176,543 to 1,177,787 lolE b1119: nagK - N-acetyl-D-glucosamine kinase (NCBI), at 1,177,816 to 1,178,727 nagK b1120: cobB - deacetylase of acs and cheY, regulates chemotaxis (NCBI), at 1,178,854 to 1,179,582 cobB b1121: ycfZ - predicted inner membrane protein (NCBI), at 1,179,702 to 1,180,490 ycfZ Position (kb) 1177 1178 1179Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1177.788 kb on + strandat 1177.791 kb on - strandat 1177.988 kb on - strand, within nagKat 1177.988 kb on - strand, within nagKat 1178.081 kb on + strand, within nagKat 1178.086 kb on + strand, within nagKat 1178.100 kb on + strand, within nagKat 1178.411 kb on - strand, within nagKat 1178.423 kb on + strand, within nagKat 1178.423 kb on + strand, within nagKat 1178.483 kb on + strand, within nagKat 1178.486 kb on - strand, within nagKat 1178.567 kb on - strand, within nagKat 1178.692 kb on - strandat 1178.693 kb on + strandat 1178.708 kb on + strandat 1178.708 kb on + strandat 1178.766 kb on + strandat 1178.766 kb on + strandat 1178.875 kb on + strandat 1178.875 kb on + strandat 1178.880 kb on - strandat 1178.880 kb on - strandat 1179.036 kb on - strand, within cobBat 1179.142 kb on + strand, within cobBat 1179.161 kb on + strand, within cobBat 1179.161 kb on + strand, within cobBat 1179.195 kb on - strand, within cobBat 1179.319 kb on + strand, within cobBat 1179.396 kb on + strand, within cobBat 1179.413 kb on + strand, within cobBat 1179.413 kb on + strand, within cobBat 1179.421 kb on - strand, within cobBat 1179.526 kb on + strandat 1179.552 kb on - strandat 1179.584 kb on + strandat 1179.584 kb on + strandat 1179.584 kb on + strandat 1179.594 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Doxycycline hyclate 0.00000625 mM
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1,177,788 + -0.4
1,177,791 - +0.0
1,177,988 - nagK b1119 0.19 -0.2
1,177,988 - nagK b1119 0.19 -0.2
1,178,081 + nagK b1119 0.29 -0.1
1,178,086 + nagK b1119 0.30 +0.1
1,178,100 + nagK b1119 0.31 -0.8
1,178,411 - nagK b1119 0.65 -0.8
1,178,423 + nagK b1119 0.67 -0.8
1,178,423 + nagK b1119 0.67 -2.4
1,178,483 + nagK b1119 0.73 -1.2
1,178,486 - nagK b1119 0.73 -0.6
1,178,567 - nagK b1119 0.82 +0.1
1,178,692 - -0.3
1,178,693 + -1.2
1,178,708 + -1.0
1,178,708 + +0.2
1,178,766 + +0.8
1,178,766 + -1.3
1,178,875 + +0.2
1,178,875 + +0.3
1,178,880 - +0.8
1,178,880 - -0.2
1,179,036 - cobB b1120 0.25 -0.8
1,179,142 + cobB b1120 0.40 +1.0
1,179,161 + cobB b1120 0.42 -1.1
1,179,161 + cobB b1120 0.42 -1.0
1,179,195 - cobB b1120 0.47 +3.1
1,179,319 + cobB b1120 0.64 -1.0
1,179,396 + cobB b1120 0.74 +0.3
1,179,413 + cobB b1120 0.77 +0.1
1,179,413 + cobB b1120 0.77 +1.2
1,179,421 - cobB b1120 0.78 -0.2
1,179,526 + -1.8
1,179,552 - +0.8
1,179,584 + +1.6
1,179,584 + +1.0
1,179,584 + -0.0
1,179,594 + -0.6

Or see this region's nucleotide sequence