Experiment: Dictyostellum sp.mixed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4735 and GFF4736 overlap by 44 nucleotides GFF4736 and GFF4737 overlap by 8 nucleotides GFF4737 and GFF4738 overlap by 4 nucleotides GFF4738 and GFF4739 are separated by 81 nucleotides GFF4739 and GFF4740 are separated by 24 nucleotides
GFF4735 - OrgB protein, associated with InvC ATPase of type III secretion system, at 43,571 to 44,251
GFF4735
GFF4736 - Oxygen-regulated invasion protein OrgA, at 44,208 to 44,786
GFF4736
GFF4737 - Type III secretion bridge between inner and outermembrane lipoprotein (YscJ,HrcJ,EscJ, PscJ), at 44,779 to 45,537
GFF4737
GFF4738 - Type III secretion system protein, at 45,534 to 45,776
GFF4738
GFF4739 - PrgI protein, at 45,858 to 46,100
GFF4739
GFF4740 - MxiG protein; Pathogenicity 1 island effector protein, at 46,125 to 47,189
GFF4740
Position (kb)
44
45
46 Strain fitness (log2 ratio)
-1
0
1 at 43.784 kb on - strand, within GFF4735 at 44.014 kb on + strand, within GFF4735 at 44.122 kb on + strand, within GFF4735 at 44.188 kb on + strand at 44.251 kb on + strand at 44.252 kb on - strand at 44.426 kb on + strand, within GFF4736 at 44.440 kb on - strand, within GFF4736 at 44.548 kb on + strand, within GFF4736 at 44.649 kb on + strand, within GFF4736 at 44.728 kb on + strand, within GFF4736 at 44.729 kb on - strand at 44.729 kb on - strand at 44.781 kb on - strand at 44.850 kb on - strand at 45.000 kb on - strand, within GFF4737 at 45.078 kb on - strand, within GFF4737 at 45.079 kb on + strand, within GFF4737 at 45.080 kb on - strand, within GFF4737 at 45.144 kb on - strand, within GFF4737 at 45.147 kb on + strand, within GFF4737 at 45.147 kb on + strand, within GFF4737 at 45.148 kb on - strand, within GFF4737 at 45.223 kb on + strand, within GFF4737 at 45.314 kb on + strand, within GFF4737 at 45.329 kb on + strand, within GFF4737 at 45.370 kb on - strand, within GFF4737 at 45.408 kb on + strand, within GFF4737 at 45.442 kb on - strand, within GFF4737 at 45.518 kb on + strand at 45.520 kb on + strand at 45.521 kb on - strand at 45.544 kb on - strand at 45.611 kb on - strand, within GFF4738 at 45.686 kb on + strand, within GFF4738 at 45.825 kb on - strand at 45.840 kb on - strand at 45.851 kb on + strand at 45.891 kb on - strand, within GFF4739 at 45.907 kb on - strand, within GFF4739 at 46.021 kb on - strand, within GFF4739 at 46.032 kb on + strand, within GFF4739 at 46.033 kb on - strand, within GFF4739 at 46.116 kb on + strand at 46.145 kb on - strand at 46.244 kb on - strand, within GFF4740 at 46.244 kb on - strand, within GFF4740 at 46.267 kb on + strand, within GFF4740 at 46.267 kb on + strand, within GFF4740 at 46.268 kb on - strand, within GFF4740 at 46.272 kb on - strand, within GFF4740 at 46.302 kb on + strand, within GFF4740 at 46.346 kb on + strand, within GFF4740 at 46.364 kb on - strand, within GFF4740
Per-strain Table
Position Strand Gene LocusTag Fraction Dictyostellum sp.mixed culture-2 remove 43,784 - GFF4735 0.31 -0.1 44,014 + GFF4735 0.65 -0.0 44,122 + GFF4735 0.81 -0.1 44,188 + +0.2 44,251 + +0.2 44,252 - -0.3 44,426 + GFF4736 0.38 -0.7 44,440 - GFF4736 0.40 -1.0 44,548 + GFF4736 0.59 +0.1 44,649 + GFF4736 0.76 -0.2 44,728 + GFF4736 0.90 -0.2 44,729 - -0.1 44,729 - +0.1 44,781 - -0.3 44,850 - -0.1 45,000 - GFF4737 0.29 +0.2 45,078 - GFF4737 0.39 +0.2 45,079 + GFF4737 0.40 -0.6 45,080 - GFF4737 0.40 -0.3 45,144 - GFF4737 0.48 -0.3 45,147 + GFF4737 0.48 +0.0 45,147 + GFF4737 0.48 +0.4 45,148 - GFF4737 0.49 -1.7 45,223 + GFF4737 0.58 -0.5 45,314 + GFF4737 0.70 -0.2 45,329 + GFF4737 0.72 -0.7 45,370 - GFF4737 0.78 -0.7 45,408 + GFF4737 0.83 +0.3 45,442 - GFF4737 0.87 -0.0 45,518 + -0.2 45,520 + +0.5 45,521 - -0.3 45,544 - -0.2 45,611 - GFF4738 0.32 -1.3 45,686 + GFF4738 0.63 +0.3 45,825 - -0.4 45,840 - -0.4 45,851 + -1.0 45,891 - GFF4739 0.14 +0.1 45,907 - GFF4739 0.20 -0.3 46,021 - GFF4739 0.67 -0.1 46,032 + GFF4739 0.72 -0.1 46,033 - GFF4739 0.72 -0.1 46,116 + -0.5 46,145 - -0.2 46,244 - GFF4740 0.11 -0.7 46,244 - GFF4740 0.11 -0.1 46,267 + GFF4740 0.13 +0.0 46,267 + GFF4740 0.13 -0.2 46,268 - GFF4740 0.13 -0.2 46,272 - GFF4740 0.14 +0.0 46,302 + GFF4740 0.17 -0.6 46,346 + GFF4740 0.21 -0.1 46,364 - GFF4740 0.22 -0.1
Or see this region's nucleotide sequence