Experiment: Dictyostellum sp.mixed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF355 and GFF356 are separated by 5 nucleotides GFF356 and GFF357 are separated by 4 nucleotides GFF357 and GFF358 are separated by 18 nucleotides GFF358 and GFF359 are separated by 54 nucleotides
GFF355 - ATP-dependent DNA helicase RecG (EC 3.6.1.-), at 74,116 to 76,197
GFF355
GFF356 - 'tRNA (guanosine(18)-2'-O)-methyltransferase (EC 2.1.1.34)' transl_table=11, at 76,203 to 76,892
GFF356
GFF357 - 'GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)' transl_table=11, at 76,897 to 79,008
GFF357
GFF358 - DNA-directed RNA polymerase omega subunit (EC 2.7.7.6), at 79,027 to 79,302
GFF358
GFF359 - Guanylate kinase (EC 2.7.4.8), at 79,357 to 79,980
GFF359
Position (kb) 76
77
78
79
80 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 76.128 kb on + strand at 76.418 kb on - strand, within GFF356 at 76.593 kb on - strand, within GFF356 at 76.899 kb on - strand at 76.970 kb on - strand at 76.987 kb on - strand at 77.002 kb on - strand at 77.005 kb on - strand at 77.062 kb on - strand at 77.202 kb on + strand, within GFF357 at 77.203 kb on - strand, within GFF357 at 79.064 kb on - strand, within GFF358 at 79.303 kb on - strand at 79.307 kb on - strand at 79.414 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dictyostellum sp.mixed culture-2 remove 76,128 + -1.8 76,418 - GFF356 0.31 -1.4 76,593 - GFF356 0.57 -0.1 76,899 - -0.3 76,970 - -1.8 76,987 - -0.2 77,002 - -0.3 77,005 - -0.3 77,062 - +0.1 77,202 + GFF357 0.14 -2.9 77,203 - GFF357 0.14 -1.4 79,064 - GFF358 0.13 -1.6 79,303 - -0.7 79,307 - -0.6 79,414 - -1.0
Or see this region's nucleotide sequence