Experiment: Dictyostellum sp.mixed culture-2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF353 and GFF354 are separated by 2 nucleotides GFF354 and GFF355 are separated by 2 nucleotides GFF355 and GFF356 are separated by 5 nucleotides GFF356 and GFF357 are separated by 4 nucleotides
GFF353 - Sodium/glutamate symport protein, at 72,054 to 73,259
GFF353
GFF354 - putative cytoplasmic protein, at 73,262 to 74,113
GFF354
GFF355 - ATP-dependent DNA helicase RecG (EC 3.6.1.-), at 74,116 to 76,197
GFF355
GFF356 - 'tRNA (guanosine(18)-2'-O)-methyltransferase (EC 2.1.1.34)' transl_table=11, at 76,203 to 76,892
GFF356
GFF357 - 'GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)' transl_table=11, at 76,897 to 79,008
GFF357
Position (kb) 74
75
76
77 Strain fitness (log2 ratio) -2
-1
0
1
2 at 73.118 kb on + strand, within GFF353 at 73.122 kb on - strand, within GFF353 at 73.158 kb on - strand at 73.440 kb on - strand, within GFF354 at 73.490 kb on + strand, within GFF354 at 73.490 kb on + strand, within GFF354 at 73.593 kb on - strand, within GFF354 at 73.814 kb on + strand, within GFF354 at 73.827 kb on + strand, within GFF354 at 73.891 kb on - strand, within GFF354 at 75.192 kb on + strand, within GFF355 at 75.430 kb on + strand, within GFF355 at 75.699 kb on + strand, within GFF355 at 76.128 kb on + strand at 76.418 kb on - strand, within GFF356 at 76.593 kb on - strand, within GFF356 at 76.899 kb on - strand at 76.970 kb on - strand at 76.987 kb on - strand at 77.002 kb on - strand at 77.005 kb on - strand at 77.062 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dictyostellum sp.mixed culture-2 remove 73,118 + GFF353 0.88 -0.0 73,122 - GFF353 0.89 -1.0 73,158 - +2.1 73,440 - GFF354 0.21 +0.3 73,490 + GFF354 0.27 +0.2 73,490 + GFF354 0.27 +0.4 73,593 - GFF354 0.39 -0.3 73,814 + GFF354 0.65 -0.0 73,827 + GFF354 0.66 +0.1 73,891 - GFF354 0.74 -0.2 75,192 + GFF355 0.52 -0.4 75,430 + GFF355 0.63 -0.9 75,699 + GFF355 0.76 -1.3 76,128 + -1.8 76,418 - GFF356 0.31 -1.4 76,593 - GFF356 0.57 -0.1 76,899 - -0.3 76,970 - -1.8 76,987 - -0.2 77,002 - -0.3 77,005 - -0.3 77,062 - +0.1
Or see this region's nucleotide sequence