Experiment: Dictyostellum sp.mixed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF703 and GFF704 are separated by 118 nucleotides GFF704 and GFF705 overlap by 4 nucleotides GFF705 and GFF706 are separated by 143 nucleotides
GFF703 - Potassium efflux system KefA protein / Small-conductance mechanosensitive channel, at 59,465 to 61,687
GFF703
GFF704 - Glutamate transport ATP-binding protein, at 61,806 to 62,528
GFF704
GFF705 - Glutamate transport membrane-spanning protein, at 62,525 to 63,184
GFF705
GFF706 - Glutamine ABC transporter, periplasmic glutamine-binding protein (TC 3.A.1.3.2), at 63,328 to 64,074
GFF706
Position (kb) 61
62
63 Strain fitness (log2 ratio) -2
-1
0
1 at 60.881 kb on - strand, within GFF703 at 61.064 kb on - strand, within GFF703 at 61.088 kb on + strand, within GFF703 at 61.209 kb on - strand, within GFF703 at 61.726 kb on - strand at 61.727 kb on + strand at 61.727 kb on + strand at 61.900 kb on + strand, within GFF704 at 62.384 kb on + strand, within GFF704 at 62.391 kb on - strand, within GFF704 at 62.391 kb on - strand, within GFF704 at 62.397 kb on + strand, within GFF704 at 62.401 kb on - strand, within GFF704 at 62.474 kb on + strand at 62.475 kb on - strand at 62.475 kb on - strand at 62.478 kb on + strand at 62.479 kb on - strand at 62.689 kb on + strand, within GFF705 at 62.714 kb on - strand, within GFF705 at 62.894 kb on - strand, within GFF705 at 63.184 kb on + strand at 63.212 kb on + strand at 63.371 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dictyostellum sp.mixed culture-1 remove 60,881 - GFF703 0.64 +0.3 61,064 - GFF703 0.72 -0.4 61,088 + GFF703 0.73 +0.2 61,209 - GFF703 0.78 +0.4 61,726 - -0.0 61,727 + -2.1 61,727 + +0.3 61,900 + GFF704 0.13 +0.5 62,384 + GFF704 0.80 +0.4 62,391 - GFF704 0.81 +0.3 62,391 - GFF704 0.81 +0.1 62,397 + GFF704 0.82 +0.0 62,401 - GFF704 0.82 +0.3 62,474 + -0.1 62,475 - -0.2 62,475 - -0.4 62,478 + +0.3 62,479 - +0.3 62,689 + GFF705 0.25 +0.1 62,714 - GFF705 0.29 +0.5 62,894 - GFF705 0.56 -0.4 63,184 + +0.2 63,212 + +0.2 63,371 + +0.2
Or see this region's nucleotide sequence