Experiment: Dictyostellum sp.mixed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3414 and GFF3415 overlap by 1 nucleotides GFF3415 and GFF3416 are separated by 123 nucleotides GFF3416 and GFF3417 are separated by 3 nucleotides GFF3417 and GFF3418 are separated by 86 nucleotides
GFF3414 - Sensor protein PhoQ (EC 2.7.13.3), at 179,278 to 180,741
GFF3414
GFF3415 - Transcriptional regulatory protein PhoP, at 180,741 to 181,415
GFF3415
GFF3416 - Adenylosuccinate lyase (EC 4.3.2.2), at 181,539 to 182,909
GFF3416
GFF3417 - FIG002903: a protein of unknown function perhaps involved in purine metabolism, at 182,913 to 183,554
GFF3417
GFF3418 - tRNA-specific 2-thiouridylase MnmA, at 183,641 to 184,747
GFF3418
Position (kb) 181
182
183 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 180.561 kb on + strand, within GFF3414 at 180.736 kb on - strand at 180.849 kb on - strand, within GFF3415 at 180.877 kb on + strand, within GFF3415 at 181.079 kb on + strand, within GFF3415 at 181.257 kb on + strand, within GFF3415 at 181.298 kb on - strand, within GFF3415 at 181.405 kb on - strand at 181.483 kb on + strand at 181.541 kb on - strand at 181.545 kb on - strand at 182.982 kb on - strand, within GFF3417 at 183.143 kb on - strand, within GFF3417 at 183.166 kb on - strand, within GFF3417 at 183.234 kb on - strand, within GFF3417 at 183.291 kb on - strand, within GFF3417 at 183.291 kb on - strand, within GFF3417 at 183.405 kb on - strand, within GFF3417 at 183.572 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Dictyostellum sp.mixed culture-1 remove 180,561 + GFF3414 0.88 -3.5 180,736 - -1.7 180,849 - GFF3415 0.16 -2.8 180,877 + GFF3415 0.20 -0.6 181,079 + GFF3415 0.50 -3.0 181,257 + GFF3415 0.76 -2.9 181,298 - GFF3415 0.83 -1.4 181,405 - -2.6 181,483 + -0.3 181,541 - +0.1 181,545 - +0.1 182,982 - GFF3417 0.11 -0.3 183,143 - GFF3417 0.36 +0.5 183,166 - GFF3417 0.39 -0.3 183,234 - GFF3417 0.50 -0.1 183,291 - GFF3417 0.59 -0.6 183,291 - GFF3417 0.59 +0.3 183,405 - GFF3417 0.77 +0.4 183,572 - +0.2
Or see this region's nucleotide sequence