Strain Fitness in Escherichia coli BW25113 around b1653

Experiment: LB Aerobic with Novobiocin 0.0000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgloA and rnt are separated by 102 nucleotidesrnt and lhr are separated by 92 nucleotideslhr and ydhD are separated by 50 nucleotidesydhD and ydhO are separated by 333 nucleotides b1651: gloA - glyoxalase I, Ni-dependent (NCBI), at 1,725,861 to 1,726,268 gloA b1652: rnt - ribonuclease T (NCBI), at 1,726,371 to 1,727,018 rnt b1653: lhr - predicted ATP-dependent helicase (NCBI), at 1,727,111 to 1,731,727 lhr b1654: ydhD - hypothetical protein (NCBI), at 1,731,778 to 1,732,125 ydhD b1655: ydhO - predicted lipoprotein (NCBI), at 1,732,459 to 1,733,274 ydhO Position (kb) 1727 1728 1729 1730 1731 1732Strain fitness (log2 ratio) -1 0 1 2at 1726.185 kb on - strand, within gloAat 1726.185 kb on - strand, within gloAat 1727.342 kb on + strandat 1727.625 kb on - strand, within lhrat 1727.670 kb on + strand, within lhrat 1727.670 kb on + strand, within lhrat 1727.696 kb on + strand, within lhrat 1727.696 kb on + strand, within lhrat 1727.730 kb on - strand, within lhrat 1727.735 kb on - strand, within lhrat 1727.739 kb on + strand, within lhrat 1727.739 kb on + strand, within lhrat 1727.925 kb on + strand, within lhrat 1728.073 kb on - strand, within lhrat 1728.073 kb on - strand, within lhrat 1728.670 kb on + strand, within lhrat 1728.761 kb on - strand, within lhrat 1728.761 kb on - strand, within lhrat 1728.763 kb on - strand, within lhrat 1728.777 kb on + strand, within lhrat 1728.777 kb on + strand, within lhrat 1728.963 kb on + strand, within lhrat 1728.982 kb on - strand, within lhrat 1729.610 kb on - strand, within lhrat 1729.777 kb on - strand, within lhrat 1729.782 kb on + strand, within lhrat 1729.998 kb on + strand, within lhrat 1730.110 kb on + strand, within lhrat 1730.221 kb on + strand, within lhrat 1730.226 kb on + strand, within lhrat 1730.429 kb on - strand, within lhrat 1731.154 kb on + strand, within lhrat 1731.316 kb on - strandat 1731.640 kb on - strandat 1731.728 kb on - strandat 1732.385 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Novobiocin 0.0000625 mM
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1,726,185 - gloA b1651 0.79 +2.2
1,726,185 - gloA b1651 0.79 +1.8
1,727,342 + +0.7
1,727,625 - lhr b1653 0.11 +0.6
1,727,670 + lhr b1653 0.12 -0.2
1,727,670 + lhr b1653 0.12 +0.0
1,727,696 + lhr b1653 0.13 +0.8
1,727,696 + lhr b1653 0.13 +1.8
1,727,730 - lhr b1653 0.13 +0.0
1,727,735 - lhr b1653 0.14 +0.1
1,727,739 + lhr b1653 0.14 -0.2
1,727,739 + lhr b1653 0.14 +1.7
1,727,925 + lhr b1653 0.18 +0.5
1,728,073 - lhr b1653 0.21 -0.1
1,728,073 - lhr b1653 0.21 -0.4
1,728,670 + lhr b1653 0.34 -0.1
1,728,761 - lhr b1653 0.36 -1.4
1,728,761 - lhr b1653 0.36 -0.9
1,728,763 - lhr b1653 0.36 +0.6
1,728,777 + lhr b1653 0.36 -0.8
1,728,777 + lhr b1653 0.36 -0.6
1,728,963 + lhr b1653 0.40 -0.5
1,728,982 - lhr b1653 0.41 +0.5
1,729,610 - lhr b1653 0.54 +0.4
1,729,777 - lhr b1653 0.58 -0.2
1,729,782 + lhr b1653 0.58 +0.4
1,729,998 + lhr b1653 0.63 +0.2
1,730,110 + lhr b1653 0.65 +0.6
1,730,221 + lhr b1653 0.67 -0.4
1,730,226 + lhr b1653 0.67 +0.8
1,730,429 - lhr b1653 0.72 +1.8
1,731,154 + lhr b1653 0.88 -0.1
1,731,316 - -0.2
1,731,640 - -0.7
1,731,728 - -0.8
1,732,385 - +0.1

Or see this region's nucleotide sequence