Experiment: LB Aerobic with Novobiocin 0.0000625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gloA and rnt are separated by 102 nucleotides rnt and lhr are separated by 92 nucleotides lhr and ydhD are separated by 50 nucleotides ydhD and ydhO are separated by 333 nucleotides
b1651: gloA - glyoxalase I, Ni-dependent (NCBI), at 1,725,861 to 1,726,268
gloA
b1652: rnt - ribonuclease T (NCBI), at 1,726,371 to 1,727,018
rnt
b1653: lhr - predicted ATP-dependent helicase (NCBI), at 1,727,111 to 1,731,727
lhr
b1654: ydhD - hypothetical protein (NCBI), at 1,731,778 to 1,732,125
ydhD
b1655: ydhO - predicted lipoprotein (NCBI), at 1,732,459 to 1,733,274
ydhO
Position (kb)
1727
1728
1729
1730
1731
1732 Strain fitness (log2 ratio)
-1
0
1
2 at 1726.185 kb on - strand, within gloA at 1726.185 kb on - strand, within gloA at 1727.342 kb on + strand at 1727.625 kb on - strand, within lhr at 1727.670 kb on + strand, within lhr at 1727.670 kb on + strand, within lhr at 1727.696 kb on + strand, within lhr at 1727.696 kb on + strand, within lhr at 1727.730 kb on - strand, within lhr at 1727.735 kb on - strand, within lhr at 1727.739 kb on + strand, within lhr at 1727.739 kb on + strand, within lhr at 1727.925 kb on + strand, within lhr at 1728.073 kb on - strand, within lhr at 1728.073 kb on - strand, within lhr at 1728.670 kb on + strand, within lhr at 1728.761 kb on - strand, within lhr at 1728.761 kb on - strand, within lhr at 1728.763 kb on - strand, within lhr at 1728.777 kb on + strand, within lhr at 1728.777 kb on + strand, within lhr at 1728.963 kb on + strand, within lhr at 1728.982 kb on - strand, within lhr at 1729.610 kb on - strand, within lhr at 1729.777 kb on - strand, within lhr at 1729.782 kb on + strand, within lhr at 1729.998 kb on + strand, within lhr at 1730.110 kb on + strand, within lhr at 1730.221 kb on + strand, within lhr at 1730.226 kb on + strand, within lhr at 1730.429 kb on - strand, within lhr at 1731.154 kb on + strand, within lhr at 1731.316 kb on - strand at 1731.640 kb on - strand at 1731.728 kb on - strand at 1732.385 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Novobiocin 0.0000625 mM remove 1,726,185 - gloA b1651 0.79 +2.2 1,726,185 - gloA b1651 0.79 +1.8 1,727,342 + +0.7 1,727,625 - lhr b1653 0.11 +0.6 1,727,670 + lhr b1653 0.12 -0.2 1,727,670 + lhr b1653 0.12 +0.0 1,727,696 + lhr b1653 0.13 +0.8 1,727,696 + lhr b1653 0.13 +1.8 1,727,730 - lhr b1653 0.13 +0.0 1,727,735 - lhr b1653 0.14 +0.1 1,727,739 + lhr b1653 0.14 -0.2 1,727,739 + lhr b1653 0.14 +1.7 1,727,925 + lhr b1653 0.18 +0.5 1,728,073 - lhr b1653 0.21 -0.1 1,728,073 - lhr b1653 0.21 -0.4 1,728,670 + lhr b1653 0.34 -0.1 1,728,761 - lhr b1653 0.36 -1.4 1,728,761 - lhr b1653 0.36 -0.9 1,728,763 - lhr b1653 0.36 +0.6 1,728,777 + lhr b1653 0.36 -0.8 1,728,777 + lhr b1653 0.36 -0.6 1,728,963 + lhr b1653 0.40 -0.5 1,728,982 - lhr b1653 0.41 +0.5 1,729,610 - lhr b1653 0.54 +0.4 1,729,777 - lhr b1653 0.58 -0.2 1,729,782 + lhr b1653 0.58 +0.4 1,729,998 + lhr b1653 0.63 +0.2 1,730,110 + lhr b1653 0.65 +0.6 1,730,221 + lhr b1653 0.67 -0.4 1,730,226 + lhr b1653 0.67 +0.8 1,730,429 - lhr b1653 0.72 +1.8 1,731,154 + lhr b1653 0.88 -0.1 1,731,316 - -0.2 1,731,640 - -0.7 1,731,728 - -0.8 1,732,385 - +0.1
Or see this region's nucleotide sequence