Experiment: LB 0.5x in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10380 and PS417_10385 are separated by 96 nucleotides PS417_10385 and PS417_10390 are separated by 35 nucleotides PS417_10390 and PS417_10395 are separated by 79 nucleotides PS417_10395 and PS417_10400 are separated by 226 nucleotides
PS417_10380: PS417_10380 - catalase, at 2,220,706 to 2,222,139
_10380
PS417_10385: PS417_10385 - peroxiredoxin, at 2,222,236 to 2,222,652
_10385
PS417_10390: PS417_10390 - RNA polymerase subunit sigma24, at 2,222,688 to 2,223,059
_10390
PS417_10395: PS417_10395 - amidase, at 2,223,139 to 2,223,918
_10395
PS417_10400: PS417_10400 - membrane protein, at 2,224,145 to 2,225,212
_10400
Position (kb)
2223
2224 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2222.215 kb on + strand at 2222.216 kb on - strand at 2222.285 kb on + strand, within PS417_10385 at 2222.523 kb on + strand, within PS417_10385 at 2222.627 kb on + strand at 2222.690 kb on - strand at 2222.690 kb on - strand at 2222.691 kb on + strand at 2222.691 kb on + strand at 2222.691 kb on + strand at 2222.692 kb on - strand at 2222.736 kb on - strand, within PS417_10390 at 2222.763 kb on - strand at 2222.908 kb on + strand, within PS417_10390 at 2222.909 kb on - strand, within PS417_10390 at 2223.192 kb on + strand at 2223.357 kb on + strand, within PS417_10395 at 2223.358 kb on - strand, within PS417_10395 at 2223.545 kb on + strand, within PS417_10395 at 2223.545 kb on + strand, within PS417_10395 at 2223.546 kb on - strand, within PS417_10395 at 2223.546 kb on - strand, within PS417_10395 at 2223.703 kb on + strand, within PS417_10395 at 2223.704 kb on - strand, within PS417_10395 at 2223.910 kb on + strand at 2223.911 kb on - strand at 2224.050 kb on - strand at 2224.050 kb on - strand at 2224.050 kb on - strand at 2224.095 kb on + strand at 2224.109 kb on + strand at 2224.151 kb on - strand at 2224.490 kb on + strand, within PS417_10400 at 2224.652 kb on + strand, within PS417_10400 at 2224.653 kb on - strand, within PS417_10400 at 2224.662 kb on - strand, within PS417_10400 at 2224.697 kb on - strand, within PS417_10400 at 2224.697 kb on - strand, within PS417_10400 at 2224.697 kb on - strand, within PS417_10400
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x in EcoFAB_3.5mL remove 2,222,215 + -0.2 2,222,216 - -0.3 2,222,285 + PS417_10385 0.12 -0.1 2,222,523 + PS417_10385 0.69 -0.8 2,222,627 + +0.9 2,222,690 - +0.5 2,222,690 - -1.3 2,222,691 + -0.0 2,222,691 + +0.3 2,222,691 + -0.3 2,222,692 - -1.3 2,222,736 - PS417_10390 0.13 +0.2 2,222,763 - +0.7 2,222,908 + PS417_10390 0.59 -0.4 2,222,909 - PS417_10390 0.59 +0.3 2,223,192 + -1.3 2,223,357 + PS417_10395 0.28 +0.1 2,223,358 - PS417_10395 0.28 -1.8 2,223,545 + PS417_10395 0.52 -0.3 2,223,545 + PS417_10395 0.52 -0.5 2,223,546 - PS417_10395 0.52 -0.2 2,223,546 - PS417_10395 0.52 -0.2 2,223,703 + PS417_10395 0.72 -1.1 2,223,704 - PS417_10395 0.72 +0.1 2,223,910 + -0.8 2,223,911 - +3.6 2,224,050 - -0.3 2,224,050 - -0.0 2,224,050 - -0.8 2,224,095 + -0.2 2,224,109 + -0.5 2,224,151 - +0.7 2,224,490 + PS417_10400 0.32 +0.0 2,224,652 + PS417_10400 0.47 -0.1 2,224,653 - PS417_10400 0.48 +0.8 2,224,662 - PS417_10400 0.48 +0.6 2,224,697 - PS417_10400 0.52 +3.9 2,224,697 - PS417_10400 0.52 +0.4 2,224,697 - PS417_10400 0.52 +0.0
Or see this region's nucleotide sequence