Strain Fitness in Escherichia coli BW25113 around b0950

Experiment: LB Aerobic with Benzethonium chloride 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntuup and pqiA are separated by 129 nucleotidespqiA and pqiB are separated by 4 nucleotides b0949: uup - fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 1,009,187 to 1,011,094 uup b0950: pqiA - paraquat-inducible membrane protein A (NCBI), at 1,011,224 to 1,012,477 pqiA b0951: pqiB - paraquat-inducible protein B (NCBI), at 1,012,482 to 1,014,122 pqiB Position (kb) 1011 1012 1013Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1010.325 kb on - strand, within uupat 1010.439 kb on + strand, within uupat 1010.589 kb on - strand, within uupat 1010.589 kb on - strand, within uupat 1010.662 kb on - strand, within uupat 1010.752 kb on - strand, within uupat 1010.782 kb on + strand, within uupat 1010.882 kb on - strand, within uupat 1010.882 kb on - strand, within uupat 1011.118 kb on + strandat 1011.607 kb on - strand, within pqiAat 1011.620 kb on - strand, within pqiAat 1011.748 kb on + strand, within pqiAat 1011.794 kb on + strand, within pqiAat 1011.876 kb on + strand, within pqiAat 1011.956 kb on - strand, within pqiAat 1011.982 kb on + strand, within pqiAat 1012.008 kb on - strand, within pqiAat 1012.008 kb on - strand, within pqiAat 1012.011 kb on + strand, within pqiAat 1012.067 kb on + strand, within pqiAat 1012.067 kb on + strand, within pqiAat 1012.131 kb on - strand, within pqiAat 1012.166 kb on - strand, within pqiAat 1012.166 kb on - strand, within pqiAat 1012.181 kb on - strand, within pqiAat 1012.260 kb on - strand, within pqiAat 1012.323 kb on - strand, within pqiAat 1012.323 kb on - strand, within pqiAat 1012.450 kb on + strandat 1012.450 kb on + strandat 1012.460 kb on + strandat 1012.570 kb on - strandat 1012.570 kb on - strandat 1012.625 kb on - strandat 1012.625 kb on - strandat 1012.672 kb on + strand, within pqiBat 1012.805 kb on + strand, within pqiBat 1012.847 kb on + strand, within pqiBat 1012.865 kb on - strand, within pqiBat 1012.865 kb on - strand, within pqiBat 1013.007 kb on + strand, within pqiBat 1013.020 kb on - strand, within pqiBat 1013.020 kb on - strand, within pqiBat 1013.031 kb on - strand, within pqiBat 1013.108 kb on - strand, within pqiBat 1013.119 kb on + strand, within pqiBat 1013.144 kb on + strand, within pqiBat 1013.168 kb on - strand, within pqiBat 1013.178 kb on - strand, within pqiBat 1013.178 kb on - strand, within pqiBat 1013.211 kb on - strand, within pqiBat 1013.329 kb on - strand, within pqiBat 1013.374 kb on + strand, within pqiBat 1013.374 kb on + strand, within pqiBat 1013.389 kb on - strand, within pqiBat 1013.399 kb on + strand, within pqiB

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Benzethonium chloride 0.000015625 mM
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1,010,325 - uup b0949 0.60 -2.4
1,010,439 + uup b0949 0.66 +0.5
1,010,589 - uup b0949 0.73 +1.0
1,010,589 - uup b0949 0.73 -0.2
1,010,662 - uup b0949 0.77 +0.1
1,010,752 - uup b0949 0.82 +1.0
1,010,782 + uup b0949 0.84 +0.5
1,010,882 - uup b0949 0.89 -1.0
1,010,882 - uup b0949 0.89 -1.7
1,011,118 + +0.4
1,011,607 - pqiA b0950 0.31 -1.3
1,011,620 - pqiA b0950 0.32 -2.8
1,011,748 + pqiA b0950 0.42 -2.1
1,011,794 + pqiA b0950 0.45 -0.8
1,011,876 + pqiA b0950 0.52 -1.3
1,011,956 - pqiA b0950 0.58 -1.5
1,011,982 + pqiA b0950 0.60 -1.6
1,012,008 - pqiA b0950 0.63 -2.8
1,012,008 - pqiA b0950 0.63 +0.2
1,012,011 + pqiA b0950 0.63 -3.2
1,012,067 + pqiA b0950 0.67 -1.5
1,012,067 + pqiA b0950 0.67 -2.5
1,012,131 - pqiA b0950 0.72 -1.0
1,012,166 - pqiA b0950 0.75 -1.6
1,012,166 - pqiA b0950 0.75 -1.3
1,012,181 - pqiA b0950 0.76 -4.0
1,012,260 - pqiA b0950 0.83 -0.8
1,012,323 - pqiA b0950 0.88 -2.1
1,012,323 - pqiA b0950 0.88 +0.2
1,012,450 + -1.1
1,012,450 + -0.2
1,012,460 + -0.3
1,012,570 - -0.8
1,012,570 - -2.1
1,012,625 - -0.8
1,012,625 - -2.3
1,012,672 + pqiB b0951 0.12 -3.1
1,012,805 + pqiB b0951 0.20 -3.8
1,012,847 + pqiB b0951 0.22 -1.6
1,012,865 - pqiB b0951 0.23 -1.8
1,012,865 - pqiB b0951 0.23 -1.3
1,013,007 + pqiB b0951 0.32 -4.5
1,013,020 - pqiB b0951 0.33 +0.2
1,013,020 - pqiB b0951 0.33 -1.1
1,013,031 - pqiB b0951 0.33 -2.8
1,013,108 - pqiB b0951 0.38 -1.3
1,013,119 + pqiB b0951 0.39 -1.8
1,013,144 + pqiB b0951 0.40 -0.3
1,013,168 - pqiB b0951 0.42 -2.0
1,013,178 - pqiB b0951 0.42 -2.1
1,013,178 - pqiB b0951 0.42 -1.1
1,013,211 - pqiB b0951 0.44 -3.9
1,013,329 - pqiB b0951 0.52 -1.8
1,013,374 + pqiB b0951 0.54 -1.6
1,013,374 + pqiB b0951 0.54 -1.6
1,013,389 - pqiB b0951 0.55 -1.8
1,013,399 + pqiB b0951 0.56 -2.3

Or see this region's nucleotide sequence