Experiment: LB in EcoFAB_3.5mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_22165 and PS417_22170 are separated by 98 nucleotides PS417_22170 and PS417_22175 are separated by 5 nucleotides PS417_22175 and PS417_22180 are separated by 178 nucleotides PS417_22180 and PS417_22185 are separated by 139 nucleotides
PS417_22165: PS417_22165 - LysR family transcriptional regulator, at 4,802,344 to 4,803,237
_22165
PS417_22170: PS417_22170 - acetyltransferase, at 4,803,336 to 4,803,737
_22170
PS417_22175: PS417_22175 - amino acid transporter LysE, at 4,803,743 to 4,804,342
_22175
PS417_22180: PS417_22180 - superoxide dismutase, at 4,804,521 to 4,805,117
_22180
PS417_22185: PS417_22185 - hypothetical protein, at 4,805,257 to 4,805,442
_22185
Position (kb) 4803
4804
4805 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4803.226 kb on - strand at 4803.280 kb on - strand at 4803.321 kb on + strand at 4803.322 kb on - strand at 4803.323 kb on + strand at 4803.324 kb on - strand at 4803.324 kb on - strand at 4803.402 kb on + strand, within PS417_22170 at 4803.402 kb on + strand, within PS417_22170 at 4803.403 kb on - strand, within PS417_22170 at 4803.403 kb on - strand, within PS417_22170 at 4803.654 kb on + strand at 4803.706 kb on + strand at 4803.706 kb on + strand at 4803.707 kb on - strand at 4803.736 kb on - strand at 4803.742 kb on + strand at 4803.743 kb on - strand at 4803.820 kb on - strand, within PS417_22175 at 4804.178 kb on + strand, within PS417_22175 at 4804.414 kb on + strand at 4805.116 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL remove 4,803,226 - -0.1 4,803,280 - +0.7 4,803,321 + -0.1 4,803,322 - -0.2 4,803,323 + +2.4 4,803,324 - -0.4 4,803,324 - +0.6 4,803,402 + PS417_22170 0.16 -0.1 4,803,402 + PS417_22170 0.16 -1.2 4,803,403 - PS417_22170 0.17 -0.1 4,803,403 - PS417_22170 0.17 +0.6 4,803,654 + +0.9 4,803,706 + -0.5 4,803,706 + -2.1 4,803,707 - -0.7 4,803,736 - -1.2 4,803,742 + -0.3 4,803,743 - +2.4 4,803,820 - PS417_22175 0.13 +0.4 4,804,178 + PS417_22175 0.72 +2.4 4,804,414 + -0.6 4,805,116 - +0.1
Or see this region's nucleotide sequence