Strain Fitness in Escherichia coli BW25113 around b1660

Experiment: LB Aerobic with Zinc Pyrithione 0.0000025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydhB and ydhC are separated by 112 nucleotidesydhC and cfa are separated by 290 nucleotides b1659: ydhB - predicted DNA-binding transcriptional regulator (NCBI), at 1,736,890 to 1,737,822 ydhB b1660: ydhC - predicted transporter (NCBI), at 1,737,935 to 1,739,146 ydhC b1661: cfa - cyclopropane fatty acyl phospholipid synthase (unsaturated-phospholipid methyltransferase) (NCBI), at 1,739,437 to 1,740,585 cfa Position (kb) 1737 1738 1739 1740Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1736.965 kb on + strandat 1737.011 kb on + strand, within ydhBat 1737.229 kb on - strand, within ydhBat 1737.332 kb on + strand, within ydhBat 1737.436 kb on - strandat 1737.689 kb on + strand, within ydhBat 1737.719 kb on + strand, within ydhBat 1738.071 kb on + strand, within ydhCat 1738.195 kb on - strand, within ydhCat 1738.195 kb on - strand, within ydhCat 1738.203 kb on + strand, within ydhCat 1738.203 kb on + strand, within ydhCat 1738.723 kb on - strand, within ydhCat 1738.723 kb on - strand, within ydhCat 1738.896 kb on - strand, within ydhCat 1738.945 kb on - strand, within ydhCat 1738.945 kb on - strand, within ydhCat 1738.966 kb on - strand, within ydhCat 1739.263 kb on + strandat 1739.431 kb on - strandat 1739.431 kb on - strandat 1739.458 kb on - strandat 1739.473 kb on - strandat 1739.487 kb on + strandat 1739.797 kb on + strand, within cfa

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Zinc Pyrithione 0.0000025 mM
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1,736,965 + +1.8
1,737,011 + ydhB b1659 0.13 -1.0
1,737,229 - ydhB b1659 0.36 +0.5
1,737,332 + ydhB b1659 0.47 -0.2
1,737,436 - +0.0
1,737,689 + ydhB b1659 0.86 -0.8
1,737,719 + ydhB b1659 0.89 -0.5
1,738,071 + ydhC b1660 0.11 -1.1
1,738,195 - ydhC b1660 0.21 -1.5
1,738,195 - ydhC b1660 0.21 +1.2
1,738,203 + ydhC b1660 0.22 +0.7
1,738,203 + ydhC b1660 0.22 +2.4
1,738,723 - ydhC b1660 0.65 +0.0
1,738,723 - ydhC b1660 0.65 -2.8
1,738,896 - ydhC b1660 0.79 -0.6
1,738,945 - ydhC b1660 0.83 -1.0
1,738,945 - ydhC b1660 0.83 -1.8
1,738,966 - ydhC b1660 0.85 -0.4
1,739,263 + +1.3
1,739,431 - -0.4
1,739,431 - +0.5
1,739,458 - -0.7
1,739,473 - -0.6
1,739,487 + +0.8
1,739,797 + cfa b1661 0.31 -0.6

Or see this region's nucleotide sequence