Strain Fitness in Escherichia coli BW25113 around b3165

Experiment: LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpnp and sraG are separated by 56 nucleotidessraG and rpsO are separated by 16 nucleotidesrpsO and truB are separated by 148 nucleotides b3164: pnp - polynucleotide phosphorylase/polyadenylase (RefSeq), at 3,307,055 to 3,309,190 pnp b4449: sraG - unknown RNA (NCBI), at 3,309,247 to 3,309,420 sraG b3165: rpsO - 30S ribosomal protein S15 (NCBI), at 3,309,437 to 3,309,706 rpsO b3166: truB - tRNA pseudouridine synthase B (NCBI), at 3,309,855 to 3,310,799 truB Position (kb) 3309 3310Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3309.216 kb on - strandat 3309.239 kb on - strandat 3309.437 kb on - strandat 3309.823 kb on - strandat 3309.823 kb on - strandat 3309.838 kb on - strandat 3309.987 kb on - strand, within truBat 3309.990 kb on - strand, within truBat 3310.065 kb on + strand, within truBat 3310.085 kb on - strand, within truBat 3310.126 kb on + strand, within truBat 3310.148 kb on + strand, within truBat 3310.148 kb on + strand, within truBat 3310.202 kb on + strand, within truBat 3310.210 kb on - strand, within truBat 3310.242 kb on + strand, within truBat 3310.371 kb on - strand, within truBat 3310.487 kb on + strand, within truBat 3310.523 kb on - strand, within truBat 3310.539 kb on + strand, within truBat 3310.545 kb on - strand, within truBat 3310.562 kb on + strand, within truBat 3310.562 kb on + strand, within truBat 3310.587 kb on + strand, within truBat 3310.655 kb on + strand, within truBat 3310.655 kb on + strand, within truBat 3310.682 kb on + strand, within truBat 3310.695 kb on - strand, within truBat 3310.700 kb on - strand, within truBat 3310.700 kb on - strand, within truB

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
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3,309,216 - +0.1
3,309,239 - +2.4
3,309,437 - -1.6
3,309,823 - +0.1
3,309,823 - -1.5
3,309,838 - -1.8
3,309,987 - truB b3166 0.14 +1.5
3,309,990 - truB b3166 0.14 +1.0
3,310,065 + truB b3166 0.22 +2.7
3,310,085 - truB b3166 0.24 +2.7
3,310,126 + truB b3166 0.29 +2.4
3,310,148 + truB b3166 0.31 -2.1
3,310,148 + truB b3166 0.31 +0.4
3,310,202 + truB b3166 0.37 +1.7
3,310,210 - truB b3166 0.38 +0.8
3,310,242 + truB b3166 0.41 +2.2
3,310,371 - truB b3166 0.55 -1.6
3,310,487 + truB b3166 0.67 +1.9
3,310,523 - truB b3166 0.71 +1.8
3,310,539 + truB b3166 0.72 +0.9
3,310,545 - truB b3166 0.73 +1.4
3,310,562 + truB b3166 0.75 -1.3
3,310,562 + truB b3166 0.75 +1.2
3,310,587 + truB b3166 0.77 -1.1
3,310,655 + truB b3166 0.85 +3.4
3,310,655 + truB b3166 0.85 +2.2
3,310,682 + truB b3166 0.88 +1.1
3,310,695 - truB b3166 0.89 +2.1
3,310,700 - truB b3166 0.89 +1.7
3,310,700 - truB b3166 0.89 +1.4

Or see this region's nucleotide sequence