Strain Fitness in Escherichia coli BW25113 around b2483

Experiment: LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthyfB and hyfC are separated by 10 nucleotideshyfC and hyfD are separated by 16 nucleotides b2482: hyfB - NADH dehydrogenase subunit N (NCBI), at 2,599,840 to 2,601,858 hyfB b2483: hyfC - hydrogenase 4 membrane subunit (VIMSS), at 2,601,869 to 2,602,816 hyfC b2484: hyfD - hydrogenase 4 membrane subunit (NCBI), at 2,602,833 to 2,604,272 hyfD Position (kb) 2601 2602 2603Strain fitness (log2 ratio) -2 -1 0 1 2at 2600.889 kb on + strand, within hyfBat 2601.194 kb on + strand, within hyfBat 2601.202 kb on - strand, within hyfBat 2601.245 kb on - strand, within hyfBat 2601.245 kb on - strand, within hyfBat 2601.404 kb on - strand, within hyfBat 2601.421 kb on + strand, within hyfBat 2601.434 kb on + strand, within hyfBat 2601.588 kb on + strand, within hyfBat 2601.644 kb on + strand, within hyfBat 2601.644 kb on + strand, within hyfBat 2601.652 kb on - strand, within hyfBat 2601.652 kb on - strand, within hyfBat 2601.701 kb on - strandat 2601.701 kb on - strandat 2601.810 kb on + strandat 2601.925 kb on + strandat 2601.925 kb on + strandat 2601.988 kb on - strand, within hyfCat 2602.127 kb on + strand, within hyfCat 2602.127 kb on + strand, within hyfCat 2602.150 kb on - strand, within hyfCat 2602.199 kb on - strand, within hyfCat 2602.199 kb on - strand, within hyfCat 2602.325 kb on + strand, within hyfCat 2602.350 kb on + strand, within hyfCat 2602.379 kb on + strand, within hyfCat 2602.382 kb on - strand, within hyfCat 2602.432 kb on - strand, within hyfCat 2602.620 kb on - strand, within hyfCat 2602.620 kb on - strand, within hyfCat 2602.813 kb on + strandat 2602.813 kb on + strandat 2603.063 kb on - strand, within hyfDat 2603.095 kb on - strand, within hyfDat 2603.151 kb on + strand, within hyfDat 2603.151 kb on + strand, within hyfDat 2603.293 kb on + strand, within hyfDat 2603.301 kb on - strand, within hyfDat 2603.328 kb on - strand, within hyfDat 2603.369 kb on + strand, within hyfDat 2603.430 kb on + strand, within hyfDat 2603.464 kb on - strand, within hyfDat 2603.464 kb on - strand, within hyfDat 2603.477 kb on - strand, within hyfDat 2603.547 kb on - strand, within hyfDat 2603.570 kb on - strand, within hyfDat 2603.574 kb on + strand, within hyfDat 2603.574 kb on + strand, within hyfDat 2603.613 kb on + strand, within hyfDat 2603.716 kb on + strand, within hyfDat 2603.726 kb on + strand, within hyfDat 2603.726 kb on + strand, within hyfDat 2603.783 kb on + strand, within hyfD

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
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2,600,889 + hyfB b2482 0.52 +0.3
2,601,194 + hyfB b2482 0.67 -0.3
2,601,202 - hyfB b2482 0.67 +2.0
2,601,245 - hyfB b2482 0.70 +0.6
2,601,245 - hyfB b2482 0.70 +2.4
2,601,404 - hyfB b2482 0.77 -1.7
2,601,421 + hyfB b2482 0.78 +2.2
2,601,434 + hyfB b2482 0.79 +0.2
2,601,588 + hyfB b2482 0.87 -0.9
2,601,644 + hyfB b2482 0.89 -2.2
2,601,644 + hyfB b2482 0.89 -1.2
2,601,652 - hyfB b2482 0.90 +0.3
2,601,652 - hyfB b2482 0.90 -0.5
2,601,701 - +0.0
2,601,701 - +0.3
2,601,810 + -1.4
2,601,925 + -2.2
2,601,925 + -1.3
2,601,988 - hyfC b2483 0.13 +0.7
2,602,127 + hyfC b2483 0.27 -1.2
2,602,127 + hyfC b2483 0.27 -2.0
2,602,150 - hyfC b2483 0.30 -1.2
2,602,199 - hyfC b2483 0.35 +1.0
2,602,199 - hyfC b2483 0.35 -0.7
2,602,325 + hyfC b2483 0.48 -1.5
2,602,350 + hyfC b2483 0.51 -0.4
2,602,379 + hyfC b2483 0.54 -0.2
2,602,382 - hyfC b2483 0.54 -1.2
2,602,432 - hyfC b2483 0.59 -0.4
2,602,620 - hyfC b2483 0.79 -1.3
2,602,620 - hyfC b2483 0.79 -1.2
2,602,813 + -2.6
2,602,813 + -0.4
2,603,063 - hyfD b2484 0.16 +0.3
2,603,095 - hyfD b2484 0.18 -1.4
2,603,151 + hyfD b2484 0.22 +0.0
2,603,151 + hyfD b2484 0.22 +1.5
2,603,293 + hyfD b2484 0.32 -0.2
2,603,301 - hyfD b2484 0.33 +1.3
2,603,328 - hyfD b2484 0.34 +2.5
2,603,369 + hyfD b2484 0.37 -2.6
2,603,430 + hyfD b2484 0.41 -0.9
2,603,464 - hyfD b2484 0.44 -2.0
2,603,464 - hyfD b2484 0.44 -1.2
2,603,477 - hyfD b2484 0.45 +1.3
2,603,547 - hyfD b2484 0.50 -1.5
2,603,570 - hyfD b2484 0.51 +1.5
2,603,574 + hyfD b2484 0.51 +2.1
2,603,574 + hyfD b2484 0.51 -1.7
2,603,613 + hyfD b2484 0.54 -0.5
2,603,716 + hyfD b2484 0.61 -0.5
2,603,726 + hyfD b2484 0.62 -0.2
2,603,726 + hyfD b2484 0.62 +0.5
2,603,783 + hyfD b2484 0.66 +0.3

Or see this region's nucleotide sequence