Strain Fitness in Escherichia coli BW25113 around b1248

Experiment: LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntoppF and b1248 are separated by 52 nucleotidesb1248 and cls are separated by 34 nucleotides b1247: oppF - oligopeptide transporter subunit (NCBI), at 1,303,788 to 1,304,792 oppF b1248: b1248 - orf, hypothetical protein (VIMSS), at 1,304,845 to 1,305,174 b1248 b1249: cls - cardiolipin synthetase (NCBI), at 1,305,209 to 1,306,669 cls Position (kb) 1304 1305 1306Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 1303.902 kb on + strand, within oppFat 1303.914 kb on - strand, within oppFat 1303.981 kb on + strand, within oppFat 1303.981 kb on + strand, within oppFat 1304.158 kb on + strand, within oppFat 1304.294 kb on + strand, within oppFat 1304.294 kb on + strand, within oppFat 1304.367 kb on + strand, within oppFat 1304.720 kb on + strandat 1304.780 kb on - strandat 1304.780 kb on - strandat 1305.078 kb on - strand, within b1248at 1305.078 kb on - strand, within b1248at 1305.176 kb on + strandat 1305.186 kb on + strandat 1305.539 kb on + strand, within clsat 1305.539 kb on + strand, within clsat 1305.576 kb on - strand, within clsat 1305.588 kb on - strand, within clsat 1305.588 kb on - strand, within clsat 1305.625 kb on - strand, within clsat 1305.641 kb on + strand, within clsat 1305.641 kb on + strand, within clsat 1305.899 kb on + strand, within clsat 1305.959 kb on + strand, within clsat 1306.053 kb on - strand, within clsat 1306.071 kb on - strand, within cls

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
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1,303,902 + oppF b1247 0.11 +0.2
1,303,914 - oppF b1247 0.13 -1.3
1,303,981 + oppF b1247 0.19 -0.5
1,303,981 + oppF b1247 0.19 +0.7
1,304,158 + oppF b1247 0.37 +1.5
1,304,294 + oppF b1247 0.50 -1.1
1,304,294 + oppF b1247 0.50 +0.9
1,304,367 + oppF b1247 0.58 -0.3
1,304,720 + -0.6
1,304,780 - -0.9
1,304,780 - -0.9
1,305,078 - b1248 b1248 0.71 +1.1
1,305,078 - b1248 b1248 0.71 -0.6
1,305,176 + -2.1
1,305,186 + +1.3
1,305,539 + cls b1249 0.23 -0.3
1,305,539 + cls b1249 0.23 -1.3
1,305,576 - cls b1249 0.25 -1.6
1,305,588 - cls b1249 0.26 -3.2
1,305,588 - cls b1249 0.26 -0.9
1,305,625 - cls b1249 0.28 -2.5
1,305,641 + cls b1249 0.30 -0.3
1,305,641 + cls b1249 0.30 -0.9
1,305,899 + cls b1249 0.47 -1.3
1,305,959 + cls b1249 0.51 -0.9
1,306,053 - cls b1249 0.58 -5.1
1,306,071 - cls b1249 0.59 -3.2

Or see this region's nucleotide sequence