Strain Fitness in Escherichia coli BW25113 around b4251

Experiment: LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyjgH and yjgI are separated by 130 nucleotidesyjgI and yjgJ are separated by 70 nucleotidesyjgJ and yjgK are separated by 144 nucleotidesyjgK and yjgL are separated by 122 nucleotides b4248: yjgH - predicted mRNA endoribonuclease (NCBI), at 4,470,837 to 4,471,232 yjgH b4249: yjgI - predicted oxidoreductase with NAD(P)-binding Rossmann-fold domain (NCBI), at 4,471,363 to 4,472,076 yjgI b4251: yjgJ - orf, hypothetical protein (VIMSS), at 4,472,147 to 4,472,740 yjgJ b4252: yjgK - orf, hypothetical protein (VIMSS), at 4,472,885 to 4,473,337 yjgK b4253: yjgL - orf, hypothetical protein (VIMSS), at 4,473,460 to 4,475,274 yjgL Position (kb) 4472 4473Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4471.176 kb on - strand, within yjgHat 4471.354 kb on - strandat 4471.362 kb on + strandat 4471.367 kb on + strandat 4471.378 kb on - strandat 4471.388 kb on - strandat 4471.388 kb on - strandat 4471.667 kb on + strand, within yjgIat 4471.680 kb on - strand, within yjgIat 4471.719 kb on + strand, within yjgIat 4471.719 kb on + strand, within yjgIat 4471.851 kb on - strand, within yjgIat 4471.851 kb on - strand, within yjgIat 4471.862 kb on - strand, within yjgIat 4471.869 kb on - strand, within yjgIat 4471.869 kb on - strand, within yjgIat 4471.909 kb on - strand, within yjgIat 4471.909 kb on - strand, within yjgIat 4471.932 kb on - strand, within yjgIat 4471.982 kb on - strand, within yjgIat 4472.190 kb on - strandat 4472.196 kb on + strandat 4472.253 kb on + strand, within yjgJat 4472.341 kb on - strand, within yjgJat 4472.352 kb on + strand, within yjgJat 4472.411 kb on + strand, within yjgJat 4472.415 kb on - strand, within yjgJat 4472.419 kb on + strand, within yjgJat 4472.419 kb on + strand, within yjgJat 4472.421 kb on + strand, within yjgJat 4472.432 kb on + strand, within yjgJat 4472.432 kb on + strand, within yjgJat 4472.454 kb on + strand, within yjgJat 4472.489 kb on + strand, within yjgJat 4472.499 kb on + strand, within yjgJat 4472.502 kb on - strand, within yjgJat 4472.568 kb on + strand, within yjgJat 4472.568 kb on + strand, within yjgJat 4472.576 kb on - strand, within yjgJat 4472.612 kb on - strand, within yjgJat 4472.645 kb on - strand, within yjgJat 4472.645 kb on - strand, within yjgJat 4472.664 kb on - strand, within yjgJat 4472.673 kb on + strand, within yjgJat 4472.675 kb on + strand, within yjgJat 4472.679 kb on - strand, within yjgJat 4472.679 kb on - strand, within yjgJat 4472.681 kb on - strandat 4472.681 kb on - strandat 4472.681 kb on - strandat 4472.681 kb on - strandat 4472.681 kb on - strandat 4472.683 kb on + strandat 4472.692 kb on - strandat 4472.702 kb on + strandat 4472.702 kb on + strandat 4472.704 kb on + strandat 4472.727 kb on + strandat 4472.727 kb on + strandat 4472.729 kb on + strandat 4472.737 kb on + strandat 4472.737 kb on + strandat 4472.739 kb on + strandat 4472.783 kb on + strandat 4472.783 kb on + strandat 4472.838 kb on + strandat 4472.838 kb on + strandat 4472.862 kb on + strandat 4472.924 kb on + strandat 4472.945 kb on + strand, within yjgKat 4472.958 kb on + strand, within yjgKat 4472.958 kb on + strand, within yjgKat 4472.958 kb on + strand, within yjgKat 4472.960 kb on + strand, within yjgKat 4472.960 kb on + strand, within yjgKat 4472.960 kb on + strand, within yjgKat 4472.999 kb on + strand, within yjgKat 4473.001 kb on - strand, within yjgKat 4473.016 kb on + strand, within yjgKat 4473.016 kb on + strand, within yjgKat 4473.034 kb on + strand, within yjgKat 4473.034 kb on + strand, within yjgKat 4473.042 kb on - strand, within yjgKat 4473.042 kb on - strand, within yjgKat 4473.049 kb on + strand, within yjgKat 4473.049 kb on + strand, within yjgKat 4473.057 kb on - strand, within yjgKat 4473.057 kb on - strand, within yjgKat 4473.057 kb on - strand, within yjgKat 4473.057 kb on - strand, within yjgKat 4473.061 kb on + strand, within yjgKat 4473.061 kb on + strand, within yjgKat 4473.070 kb on + strand, within yjgKat 4473.077 kb on - strand, within yjgKat 4473.077 kb on - strand, within yjgKat 4473.078 kb on - strand, within yjgKat 4473.127 kb on - strand, within yjgKat 4473.136 kb on + strand, within yjgKat 4473.136 kb on + strand, within yjgKat 4473.139 kb on + strand, within yjgKat 4473.139 kb on + strand, within yjgKat 4473.147 kb on + strand, within yjgKat 4473.147 kb on + strand, within yjgKat 4473.147 kb on - strand, within yjgKat 4473.163 kb on + strand, within yjgKat 4473.263 kb on + strand, within yjgKat 4473.263 kb on + strand, within yjgKat 4473.265 kb on - strand, within yjgKat 4473.273 kb on - strand, within yjgKat 4473.282 kb on + strand, within yjgKat 4473.284 kb on + strand, within yjgKat 4473.284 kb on + strand, within yjgKat 4473.286 kb on + strand, within yjgKat 4473.286 kb on + strand, within yjgKat 4473.291 kb on + strand, within yjgKat 4473.291 kb on + strand, within yjgKat 4473.296 kb on + strandat 4473.299 kb on - strandat 4473.304 kb on + strandat 4473.304 kb on + strandat 4473.311 kb on + strandat 4473.314 kb on + strandat 4473.314 kb on + strandat 4473.314 kb on + strandat 4473.426 kb on + strandat 4473.426 kb on + strandat 4473.437 kb on + strandat 4473.445 kb on + strandat 4473.583 kb on + strandat 4473.588 kb on + strandat 4473.613 kb on - strandat 4473.649 kb on + strand, within yjgLat 4473.695 kb on + strand, within yjgLat 4473.695 kb on + strand, within yjgLat 4473.695 kb on + strand, within yjgLat 4473.710 kb on + strand, within yjgLat 4473.733 kb on + strand, within yjgL

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
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4,471,176 - yjgH b4248 0.86 +0.4
4,471,354 - -1.2
4,471,362 + +1.2
4,471,367 + +0.5
4,471,378 - -0.1
4,471,388 - +0.5
4,471,388 - +1.9
4,471,667 + yjgI b4249 0.43 +0.4
4,471,680 - yjgI b4249 0.44 -0.1
4,471,719 + yjgI b4249 0.50 -2.5
4,471,719 + yjgI b4249 0.50 +1.8
4,471,851 - yjgI b4249 0.68 -0.0
4,471,851 - yjgI b4249 0.68 +0.6
4,471,862 - yjgI b4249 0.70 -0.2
4,471,869 - yjgI b4249 0.71 +0.7
4,471,869 - yjgI b4249 0.71 +1.2
4,471,909 - yjgI b4249 0.76 -1.4
4,471,909 - yjgI b4249 0.76 +0.9
4,471,932 - yjgI b4249 0.80 +0.4
4,471,982 - yjgI b4249 0.87 -1.2
4,472,190 - +0.7
4,472,196 + +0.6
4,472,253 + yjgJ b4251 0.18 +0.0
4,472,341 - yjgJ b4251 0.33 +0.8
4,472,352 + yjgJ b4251 0.35 +0.0
4,472,411 + yjgJ b4251 0.44 +1.2
4,472,415 - yjgJ b4251 0.45 -0.4
4,472,419 + yjgJ b4251 0.46 -0.9
4,472,419 + yjgJ b4251 0.46 +0.5
4,472,421 + yjgJ b4251 0.46 -0.8
4,472,432 + yjgJ b4251 0.48 -2.4
4,472,432 + yjgJ b4251 0.48 -0.4
4,472,454 + yjgJ b4251 0.52 -0.7
4,472,489 + yjgJ b4251 0.58 +0.6
4,472,499 + yjgJ b4251 0.59 -0.4
4,472,502 - yjgJ b4251 0.60 -1.6
4,472,568 + yjgJ b4251 0.71 -0.8
4,472,568 + yjgJ b4251 0.71 -0.4
4,472,576 - yjgJ b4251 0.72 -1.5
4,472,612 - yjgJ b4251 0.78 +1.0
4,472,645 - yjgJ b4251 0.84 -1.4
4,472,645 - yjgJ b4251 0.84 -0.4
4,472,664 - yjgJ b4251 0.87 -0.4
4,472,673 + yjgJ b4251 0.89 -0.6
4,472,675 + yjgJ b4251 0.89 -0.0
4,472,679 - yjgJ b4251 0.90 -0.4
4,472,679 - yjgJ b4251 0.90 -1.1
4,472,681 - +2.8
4,472,681 - +0.8
4,472,681 - +0.2
4,472,681 - +1.2
4,472,681 - -0.1
4,472,683 + +1.5
4,472,692 - -0.4
4,472,702 + -2.5
4,472,702 + -0.5
4,472,704 + +0.3
4,472,727 + +0.0
4,472,727 + -0.5
4,472,729 + +0.3
4,472,737 + +1.2
4,472,737 + -0.3
4,472,739 + +0.1
4,472,783 + +0.6
4,472,783 + -0.5
4,472,838 + +0.1
4,472,838 + +0.8
4,472,862 + -0.1
4,472,924 + -0.1
4,472,945 + yjgK b4252 0.13 +0.4
4,472,958 + yjgK b4252 0.16 -0.5
4,472,958 + yjgK b4252 0.16 +0.4
4,472,958 + yjgK b4252 0.16 +0.2
4,472,960 + yjgK b4252 0.17 +0.2
4,472,960 + yjgK b4252 0.17 -0.4
4,472,960 + yjgK b4252 0.17 +1.7
4,472,999 + yjgK b4252 0.25 +0.3
4,473,001 - yjgK b4252 0.26 -0.0
4,473,016 + yjgK b4252 0.29 -0.6
4,473,016 + yjgK b4252 0.29 -0.6
4,473,034 + yjgK b4252 0.33 -1.1
4,473,034 + yjgK b4252 0.33 -1.8
4,473,042 - yjgK b4252 0.35 +0.2
4,473,042 - yjgK b4252 0.35 +0.2
4,473,049 + yjgK b4252 0.36 -1.9
4,473,049 + yjgK b4252 0.36 -0.0
4,473,057 - yjgK b4252 0.38 +0.2
4,473,057 - yjgK b4252 0.38 +1.6
4,473,057 - yjgK b4252 0.38 -0.2
4,473,057 - yjgK b4252 0.38 -0.2
4,473,061 + yjgK b4252 0.39 +1.2
4,473,061 + yjgK b4252 0.39 -1.4
4,473,070 + yjgK b4252 0.41 -0.0
4,473,077 - yjgK b4252 0.42 -1.8
4,473,077 - yjgK b4252 0.42 +1.2
4,473,078 - yjgK b4252 0.43 -0.8
4,473,127 - yjgK b4252 0.53 +1.2
4,473,136 + yjgK b4252 0.55 -0.0
4,473,136 + yjgK b4252 0.55 +0.2
4,473,139 + yjgK b4252 0.56 +0.8
4,473,139 + yjgK b4252 0.56 +0.3
4,473,147 + yjgK b4252 0.58 +0.8
4,473,147 + yjgK b4252 0.58 -0.2
4,473,147 - yjgK b4252 0.58 +0.8
4,473,163 + yjgK b4252 0.61 -0.4
4,473,263 + yjgK b4252 0.83 -0.1
4,473,263 + yjgK b4252 0.83 +0.1
4,473,265 - yjgK b4252 0.84 +1.2
4,473,273 - yjgK b4252 0.86 +1.2
4,473,282 + yjgK b4252 0.88 +0.4
4,473,284 + yjgK b4252 0.88 +0.1
4,473,284 + yjgK b4252 0.88 +1.2
4,473,286 + yjgK b4252 0.89 +0.4
4,473,286 + yjgK b4252 0.89 -0.4
4,473,291 + yjgK b4252 0.90 -0.0
4,473,291 + yjgK b4252 0.90 -0.6
4,473,296 + -0.4
4,473,299 - +1.2
4,473,304 + +0.2
4,473,304 + +0.5
4,473,311 + +0.4
4,473,314 + -0.4
4,473,314 + +0.3
4,473,314 + +0.0
4,473,426 + +0.2
4,473,426 + +0.3
4,473,437 + +0.1
4,473,445 + -0.0
4,473,583 + +1.2
4,473,588 + +1.2
4,473,613 - +1.2
4,473,649 + yjgL b4253 0.10 +1.2
4,473,695 + yjgL b4253 0.13 +1.2
4,473,695 + yjgL b4253 0.13 +1.2
4,473,695 + yjgL b4253 0.13 +1.2
4,473,710 + yjgL b4253 0.14 +1.2
4,473,733 + yjgL b4253 0.15 -0.4

Or see this region's nucleotide sequence