Strain Fitness in Escherichia coli BW25113 around b3350
Experiment: LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
Position | Strand | Gene | LocusTag | Fraction | LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM |
---|---|---|---|---|---|
remove | |||||
3,475,861 | + | +0.6 | |||
3,475,874 | + | -0.6 | |||
3,475,874 | + | +0.8 | |||
3,475,939 | - | -1.9 | |||
3,475,971 | + | -2.0 | |||
3,475,991 | - | slyD | b3349 | 0.10 | +0.6 |
3,475,991 | - | slyD | b3349 | 0.10 | -2.0 |
3,476,000 | - | slyD | b3349 | 0.12 | +0.4 |
3,476,042 | + | +0.4 | |||
3,476,042 | + | slyD | b3349 | 0.19 | -1.0 |
3,476,061 | + | slyD | b3349 | 0.22 | +0.1 |
3,476,061 | + | slyD | b3349 | 0.22 | -2.1 |
3,476,063 | + | slyD | b3349 | 0.23 | -1.8 |
3,476,063 | + | slyD | b3349 | 0.23 | +0.8 |
3,476,089 | - | slyD | b3349 | 0.27 | -1.8 |
3,476,089 | - | slyD | b3349 | 0.27 | -2.0 |
3,476,114 | - | slyD | b3349 | 0.31 | +0.1 |
3,476,143 | + | slyD | b3349 | 0.36 | -0.9 |
3,476,206 | + | slyD | b3349 | 0.47 | +0.9 |
3,476,212 | - | slyD | b3349 | 0.48 | -0.7 |
3,476,242 | + | slyD | b3349 | 0.53 | -1.5 |
3,476,286 | + | slyD | b3349 | 0.60 | -3.0 |
3,476,333 | - | slyD | b3349 | 0.68 | -1.9 |
3,476,378 | - | slyD | b3349 | 0.76 | -2.5 |
3,476,423 | - | slyD | b3349 | 0.84 | -1.8 |
3,476,423 | - | slyD | b3349 | 0.84 | -1.4 |
3,476,465 | + | -0.2 | |||
3,476,473 | - | -1.3 | |||
3,476,473 | - | +0.0 | |||
3,476,479 | + | -0.1 | |||
3,476,479 | + | +0.6 | |||
3,476,485 | - | -0.4 | |||
3,476,485 | - | -1.0 | |||
3,476,522 | - | -1.2 | |||
3,476,525 | + | -2.4 | |||
3,476,525 | + | -2.1 | |||
3,476,525 | + | -2.9 | |||
3,476,525 | + | -0.6 | |||
3,476,544 | - | -0.6 | |||
3,476,544 | - | -0.4 | |||
3,476,549 | - | +1.0 | |||
3,476,612 | - | +0.9 | |||
3,476,649 | - | yheV | b4551 | 0.17 | -0.0 |
3,476,768 | - | yheV | b4551 | 0.77 | +1.6 |
3,476,810 | + | +1.4 | |||
3,476,853 | - | +1.6 | |||
3,476,917 | - | +1.6 | |||
3,476,917 | - | +1.3 | |||
3,476,933 | + | +1.2 | |||
3,476,941 | - | -1.0 | |||
3,476,941 | - | +1.7 | |||
3,476,941 | - | +1.2 | |||
3,476,972 | - | +1.1 | |||
3,477,015 | - | kefB | b3350 | 0.11 | +0.1 |
3,477,021 | - | +1.8 | |||
3,477,047 | - | kefB | b3350 | 0.12 | +1.3 |
3,477,096 | + | kefB | b3350 | 0.15 | +0.5 |
3,477,096 | + | kefB | b3350 | 0.15 | -0.2 |
3,477,101 | + | kefB | b3350 | 0.15 | +0.8 |
3,477,101 | + | kefB | b3350 | 0.15 | +0.6 |
3,477,109 | - | kefB | b3350 | 0.16 | +2.5 |
3,477,109 | - | kefB | b3350 | 0.16 | +1.9 |
3,477,111 | + | kefB | b3350 | 0.16 | -2.2 |
3,477,111 | + | kefB | b3350 | 0.16 | +1.0 |
3,477,113 | - | kefB | b3350 | 0.16 | -0.6 |
3,477,113 | - | kefB | b3350 | 0.16 | +1.1 |
3,477,129 | - | +1.4 | |||
3,477,129 | - | kefB | b3350 | 0.17 | +0.8 |
3,477,131 | + | kefB | b3350 | 0.17 | +0.5 |
3,477,131 | - | kefB | b3350 | 0.17 | +2.6 |
3,477,131 | - | kefB | b3350 | 0.17 | +1.5 |
3,477,164 | - | kefB | b3350 | 0.19 | +0.6 |
3,477,164 | - | kefB | b3350 | 0.19 | -0.2 |
3,477,189 | + | kefB | b3350 | 0.20 | +0.6 |
3,477,189 | + | kefB | b3350 | 0.20 | +0.2 |
3,477,197 | - | kefB | b3350 | 0.21 | +0.5 |
3,477,197 | - | kefB | b3350 | 0.21 | +1.4 |
3,477,201 | + | kefB | b3350 | 0.21 | -0.2 |
3,477,218 | - | kefB | b3350 | 0.22 | +1.5 |
3,477,219 | - | kefB | b3350 | 0.22 | +1.4 |
3,477,256 | + | kefB | b3350 | 0.24 | +0.5 |
3,477,256 | + | kefB | b3350 | 0.24 | -0.5 |
3,477,271 | - | kefB | b3350 | 0.25 | +1.2 |
3,477,271 | - | kefB | b3350 | 0.25 | -0.3 |
3,477,313 | - | kefB | b3350 | 0.27 | +1.4 |
3,477,313 | - | kefB | b3350 | 0.27 | +1.9 |
3,477,326 | + | kefB | b3350 | 0.28 | +0.8 |
3,477,337 | - | kefB | b3350 | 0.28 | +1.2 |
3,477,337 | - | kefB | b3350 | 0.28 | +2.4 |
3,477,350 | - | kefB | b3350 | 0.29 | +1.0 |
3,477,388 | + | kefB | b3350 | 0.31 | -0.1 |
3,477,417 | - | kefB | b3350 | 0.33 | -0.2 |
3,477,506 | + | kefB | b3350 | 0.38 | +1.4 |
3,477,515 | - | kefB | b3350 | 0.38 | +1.0 |
3,477,574 | + | kefB | b3350 | 0.42 | -0.0 |
3,477,579 | + | kefB | b3350 | 0.42 | +0.3 |
3,477,617 | + | kefB | b3350 | 0.44 | +0.1 |
3,477,617 | + | kefB | b3350 | 0.44 | -1.0 |
3,477,634 | - | kefB | b3350 | 0.45 | +1.2 |
3,477,636 | + | kefB | b3350 | 0.45 | +0.6 |
3,477,668 | + | kefB | b3350 | 0.47 | +0.7 |
3,477,678 | + | kefB | b3350 | 0.47 | +0.3 |
3,477,688 | - | kefB | b3350 | 0.48 | +0.7 |
3,477,724 | + | kefB | b3350 | 0.50 | +1.2 |
3,477,727 | + | kefB | b3350 | 0.50 | +0.7 |
3,477,735 | - | kefB | b3350 | 0.50 | +1.2 |
3,477,752 | - | kefB | b3350 | 0.51 | +2.4 |
3,477,757 | - | kefB | b3350 | 0.52 | +0.1 |
3,477,757 | - | kefB | b3350 | 0.52 | +1.4 |
3,477,757 | - | kefB | b3350 | 0.52 | +2.4 |
3,477,762 | - | kefB | b3350 | 0.52 | +0.4 |
3,477,764 | + | kefB | b3350 | 0.52 | -0.4 |
3,477,764 | + | kefB | b3350 | 0.52 | +0.8 |
3,477,764 | + | kefB | b3350 | 0.52 | +0.4 |
3,477,772 | - | kefB | b3350 | 0.52 | +0.7 |
3,477,772 | - | kefB | b3350 | 0.52 | +0.3 |
3,477,772 | - | kefB | b3350 | 0.52 | +1.1 |
3,477,772 | - | kefB | b3350 | 0.52 | +0.5 |
3,477,827 | + | kefB | b3350 | 0.56 | +0.7 |
3,477,827 | + | kefB | b3350 | 0.56 | +0.9 |
3,477,827 | + | kefB | b3350 | 0.56 | -0.4 |
3,477,835 | + | kefB | b3350 | 0.56 | +1.4 |
3,477,863 | - | kefB | b3350 | 0.58 | -0.1 |
3,477,863 | - | kefB | b3350 | 0.58 | +0.8 |
3,477,921 | + | kefB | b3350 | 0.61 | +0.7 |
3,477,956 | + | kefB | b3350 | 0.63 | +0.0 |
3,477,982 | + | kefB | b3350 | 0.64 | -1.4 |
3,477,982 | + | kefB | b3350 | 0.64 | -1.8 |
3,477,993 | + | kefB | b3350 | 0.65 | +0.1 |
3,478,001 | - | kefB | b3350 | 0.65 | +0.9 |
3,478,001 | - | kefB | b3350 | 0.65 | +1.4 |
3,478,001 | - | kefB | b3350 | 0.65 | -2.1 |
3,478,062 | - | kefB | b3350 | 0.69 | +1.2 |
3,478,062 | - | kefB | b3350 | 0.69 | +1.4 |
3,478,071 | + | kefB | b3350 | 0.69 | +0.6 |
3,478,079 | - | kefB | b3350 | 0.69 | +0.9 |
3,478,082 | + | kefB | b3350 | 0.70 | +0.9 |
3,478,099 | - | kefB | b3350 | 0.71 | +0.2 |
3,478,118 | + | kefB | b3350 | 0.72 | +1.4 |
3,478,130 | + | kefB | b3350 | 0.72 | -0.1 |
3,478,135 | + | kefB | b3350 | 0.73 | -1.8 |
3,478,135 | + | kefB | b3350 | 0.73 | +0.5 |
3,478,138 | - | kefB | b3350 | 0.73 | -2.7 |
3,478,223 | + | kefB | b3350 | 0.77 | +0.0 |
3,478,223 | + | kefB | b3350 | 0.77 | -0.1 |
3,478,247 | + | kefB | b3350 | 0.79 | -0.3 |
3,478,255 | - | kefB | b3350 | 0.79 | +1.7 |
3,478,255 | - | kefB | b3350 | 0.79 | +1.2 |
3,478,272 | - | kefB | b3350 | 0.80 | +0.1 |
3,478,279 | - | kefB | b3350 | 0.81 | +1.6 |
3,478,279 | - | kefB | b3350 | 0.81 | +1.5 |
3,478,291 | - | kefB | b3350 | 0.81 | +0.0 |
3,478,291 | - | kefB | b3350 | 0.81 | -0.6 |
3,478,408 | - | kefB | b3350 | 0.88 | +0.4 |
3,478,427 | - | kefB | b3350 | 0.89 | -0.2 |
3,478,440 | - | kefB | b3350 | 0.89 | +0.4 |
3,478,470 | - | +0.8 | |||
3,478,494 | - | +0.5 | |||
3,478,528 | + | +1.4 | |||
3,478,547 | - | +0.7 | |||
3,478,576 | + | +0.4 | |||
3,478,578 | - | +0.7 | |||
3,478,578 | - | -0.1 | |||
3,478,637 | + | -0.3 | |||
3,478,639 | + | +1.2 | |||
3,478,639 | + | +0.8 | |||
3,478,669 | + | -0.1 | |||
3,478,680 | + | +0.4 | |||
3,478,694 | + | kefG | b3351 | 0.12 | -0.2 |
3,478,694 | + | kefG | b3351 | 0.12 | +0.4 |
3,478,736 | + | kefG | b3351 | 0.19 | -0.6 |
3,478,755 | + | kefG | b3351 | 0.23 | -0.6 |
3,478,755 | + | kefG | b3351 | 0.23 | -0.1 |
3,478,780 | + | kefG | b3351 | 0.27 | -0.8 |
3,478,780 | + | kefG | b3351 | 0.27 | +1.5 |
3,478,780 | - | kefG | b3351 | 0.27 | -0.5 |
3,478,780 | - | kefG | b3351 | 0.27 | -0.4 |
3,478,794 | + | kefG | b3351 | 0.30 | -1.3 |
3,478,794 | + | kefG | b3351 | 0.30 | -2.3 |
3,478,819 | + | kefG | b3351 | 0.34 | +0.3 |
3,478,845 | + | kefG | b3351 | 0.39 | +0.7 |
3,478,869 | - | kefG | b3351 | 0.43 | +1.0 |
3,478,937 | - | kefG | b3351 | 0.55 | +0.0 |
3,478,941 | + | kefG | b3351 | 0.56 | -0.2 |
3,478,944 | + | kefG | b3351 | 0.57 | +0.9 |
3,478,965 | + | kefG | b3351 | 0.61 | +0.4 |
3,478,965 | + | kefG | b3351 | 0.61 | -2.1 |
3,478,966 | + | kefG | b3351 | 0.61 | -0.3 |
3,478,966 | + | kefG | b3351 | 0.61 | +0.0 |
3,478,988 | + | kefG | b3351 | 0.65 | +1.1 |
3,478,988 | + | kefG | b3351 | 0.65 | -0.2 |
3,479,017 | - | kefG | b3351 | 0.70 | -1.9 |
3,479,017 | - | kefG | b3351 | 0.70 | -1.5 |
3,479,057 | + | kefG | b3351 | 0.77 | -2.2 |
3,479,057 | + | kefG | b3351 | 0.77 | +0.6 |
3,479,080 | + | kefG | b3351 | 0.81 | -0.2 |
3,479,085 | + | kefG | b3351 | 0.82 | +0.4 |
3,479,088 | - | kefG | b3351 | 0.83 | +1.1 |
3,479,098 | + | kefG | b3351 | 0.85 | -1.2 |
3,479,108 | + | kefG | b3351 | 0.86 | -1.5 |
3,479,154 | - | -0.3 | |||
3,479,170 | + | -0.2 | |||
3,479,178 | - | +0.0 | |||
3,479,178 | - | -0.0 | |||
3,479,205 | + | -0.3 | |||
3,479,205 | + | -0.1 | |||
3,479,205 | + | +0.4 | |||
3,479,305 | - | -0.1 | |||
3,479,305 | - | +1.0 | |||
3,479,437 | - | +0.1 | |||
3,479,437 | - | +1.6 | |||
3,479,445 | - | +0.7 | |||
3,479,445 | - | +0.9 | |||
3,479,486 | + | +0.6 | |||
3,479,599 | + | yheS | b3352 | 0.15 | +0.6 |
3,479,607 | - | yheS | b3352 | 0.15 | -0.4 |
3,479,607 | - | yheS | b3352 | 0.15 | -0.8 |
3,479,628 | - | yheS | b3352 | 0.17 | -1.0 |
Or see this region's nucleotide sequence