Strain Fitness in Escherichia coli BW25113 around b3243

Experiment: LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntaaeA and yhcR are separated by 7 nucleotidesyhcR and aaeR are separated by 182 nucleotidesaaeR and tldD are separated by 133 nucleotides b3241: aaeA - p-hydroxybenzoic acid efflux system component (NCBI), at 3,386,216 to 3,387,148 aaeA b3242: yhcR - orf, hypothetical protein (VIMSS), at 3,387,156 to 3,387,359 yhcR b3243: aaeR - predicted DNA-binding transcriptional regulator, efflux system (NCBI), at 3,387,542 to 3,388,471 aaeR b3244: tldD - predicted peptidase (NCBI), at 3,388,605 to 3,390,050 tldD Position (kb) 3387 3388 3389Strain fitness (log2 ratio) -2 -1 0 1 2at 3386.569 kb on + strand, within aaeAat 3386.633 kb on + strand, within aaeAat 3386.634 kb on + strand, within aaeAat 3386.637 kb on - strand, within aaeAat 3386.642 kb on - strand, within aaeAat 3386.642 kb on - strand, within aaeAat 3386.655 kb on + strand, within aaeAat 3386.675 kb on + strand, within aaeAat 3386.763 kb on + strand, within aaeAat 3386.763 kb on + strand, within aaeAat 3386.803 kb on - strand, within aaeAat 3386.803 kb on - strand, within aaeAat 3386.803 kb on - strand, within aaeAat 3386.803 kb on - strand, within aaeAat 3386.805 kb on - strand, within aaeAat 3386.858 kb on + strand, within aaeAat 3386.882 kb on - strand, within aaeAat 3386.918 kb on + strand, within aaeAat 3386.958 kb on - strand, within aaeAat 3387.015 kb on + strand, within aaeAat 3387.015 kb on + strand, within aaeAat 3387.023 kb on - strand, within aaeAat 3387.045 kb on - strand, within aaeAat 3387.051 kb on + strand, within aaeAat 3387.051 kb on + strand, within aaeAat 3387.107 kb on + strandat 3387.200 kb on - strand, within yhcRat 3387.200 kb on - strand, within yhcRat 3387.213 kb on - strand, within yhcRat 3387.213 kb on - strand, within yhcRat 3387.213 kb on - strand, within yhcRat 3387.215 kb on - strand, within yhcRat 3387.237 kb on + strand, within yhcRat 3387.237 kb on + strand, within yhcRat 3387.242 kb on - strand, within yhcRat 3387.242 kb on - strand, within yhcRat 3387.245 kb on + strand, within yhcRat 3387.264 kb on + strand, within yhcRat 3387.264 kb on + strand, within yhcRat 3387.334 kb on - strand, within yhcRat 3387.365 kb on - strandat 3387.370 kb on + strandat 3387.372 kb on + strandat 3387.390 kb on - strandat 3387.431 kb on - strandat 3387.493 kb on - strandat 3387.495 kb on - strandat 3387.505 kb on - strandat 3387.571 kb on + strandat 3387.571 kb on + strandat 3387.614 kb on - strandat 3387.705 kb on + strand, within aaeRat 3387.775 kb on - strand, within aaeRat 3387.784 kb on - strand, within aaeRat 3387.784 kb on - strand, within aaeRat 3387.811 kb on + strand, within aaeRat 3387.827 kb on - strand, within aaeRat 3387.843 kb on - strand, within aaeRat 3387.843 kb on - strand, within aaeRat 3388.115 kb on - strand, within aaeRat 3388.115 kb on - strand, within aaeRat 3388.423 kb on + strandat 3388.478 kb on - strandat 3388.483 kb on + strandat 3388.610 kb on - strandat 3388.702 kb on + strandat 3388.702 kb on + strandat 3388.750 kb on - strand, within tldDat 3388.765 kb on + strand, within tldDat 3388.765 kb on + strand, within tldDat 3388.858 kb on + strand, within tldDat 3388.866 kb on - strand, within tldDat 3388.896 kb on + strand, within tldDat 3388.946 kb on + strand, within tldDat 3388.946 kb on + strand, within tldDat 3388.946 kb on + strand, within tldDat 3388.946 kb on + strand, within tldDat 3388.948 kb on + strand, within tldDat 3388.948 kb on + strand, within tldDat 3388.954 kb on - strand, within tldDat 3388.954 kb on - strand, within tldDat 3388.962 kb on - strand, within tldDat 3388.962 kb on - strand, within tldDat 3388.998 kb on - strand, within tldDat 3389.049 kb on - strand, within tldDat 3389.049 kb on - strand, within tldDat 3389.051 kb on + strand, within tldDat 3389.090 kb on - strand, within tldDat 3389.109 kb on - strand, within tldDat 3389.167 kb on + strand, within tldDat 3389.167 kb on + strand, within tldDat 3389.172 kb on - strand, within tldDat 3389.174 kb on + strand, within tldDat 3389.174 kb on + strand, within tldDat 3389.174 kb on + strand, within tldDat 3389.182 kb on - strand, within tldDat 3389.182 kb on - strand, within tldDat 3389.242 kb on + strand, within tldDat 3389.332 kb on + strand, within tldDat 3389.332 kb on + strand, within tldDat 3389.350 kb on + strand, within tldD

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
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3,386,569 + aaeA b3241 0.38 +0.8
3,386,633 + aaeA b3241 0.45 -0.7
3,386,634 + aaeA b3241 0.45 +0.6
3,386,637 - aaeA b3241 0.45 -0.1
3,386,642 - aaeA b3241 0.46 -1.6
3,386,642 - aaeA b3241 0.46 +1.3
3,386,655 + aaeA b3241 0.47 +0.3
3,386,675 + aaeA b3241 0.49 +0.7
3,386,763 + aaeA b3241 0.59 -0.4
3,386,763 + aaeA b3241 0.59 -0.1
3,386,803 - aaeA b3241 0.63 +0.2
3,386,803 - aaeA b3241 0.63 -0.3
3,386,803 - aaeA b3241 0.63 -0.2
3,386,803 - aaeA b3241 0.63 -0.2
3,386,805 - aaeA b3241 0.63 +0.4
3,386,858 + aaeA b3241 0.69 +0.7
3,386,882 - aaeA b3241 0.71 -0.4
3,386,918 + aaeA b3241 0.75 +0.6
3,386,958 - aaeA b3241 0.80 +0.1
3,387,015 + aaeA b3241 0.86 +1.3
3,387,015 + aaeA b3241 0.86 +0.3
3,387,023 - aaeA b3241 0.86 +0.1
3,387,045 - aaeA b3241 0.89 +1.0
3,387,051 + aaeA b3241 0.89 -0.3
3,387,051 + aaeA b3241 0.89 +0.7
3,387,107 + +0.0
3,387,200 - yhcR b3242 0.22 -1.4
3,387,200 - yhcR b3242 0.22 -0.6
3,387,213 - yhcR b3242 0.28 -1.0
3,387,213 - yhcR b3242 0.28 -2.0
3,387,213 - yhcR b3242 0.28 -0.5
3,387,215 - yhcR b3242 0.29 -0.2
3,387,237 + yhcR b3242 0.40 -0.5
3,387,237 + yhcR b3242 0.40 +1.7
3,387,242 - yhcR b3242 0.42 -1.9
3,387,242 - yhcR b3242 0.42 -0.1
3,387,245 + yhcR b3242 0.44 -0.5
3,387,264 + yhcR b3242 0.53 +1.0
3,387,264 + yhcR b3242 0.53 +0.3
3,387,334 - yhcR b3242 0.87 -0.0
3,387,365 - -1.4
3,387,370 + +0.9
3,387,372 + +1.3
3,387,390 - -1.0
3,387,431 - +0.1
3,387,493 - -0.0
3,387,495 - +0.0
3,387,505 - -0.4
3,387,571 + -0.3
3,387,571 + +0.9
3,387,614 - -0.4
3,387,705 + aaeR b3243 0.18 +0.5
3,387,775 - aaeR b3243 0.25 -1.3
3,387,784 - aaeR b3243 0.26 +1.3
3,387,784 - aaeR b3243 0.26 -0.1
3,387,811 + aaeR b3243 0.29 -0.2
3,387,827 - aaeR b3243 0.31 +0.1
3,387,843 - aaeR b3243 0.32 -0.6
3,387,843 - aaeR b3243 0.32 -0.5
3,388,115 - aaeR b3243 0.62 -0.5
3,388,115 - aaeR b3243 0.62 +0.9
3,388,423 + +0.7
3,388,478 - -0.7
3,388,483 + -0.0
3,388,610 - +0.6
3,388,702 + +0.6
3,388,702 + -0.1
3,388,750 - tldD b3244 0.10 +0.7
3,388,765 + tldD b3244 0.11 +2.3
3,388,765 + tldD b3244 0.11 +1.3
3,388,858 + tldD b3244 0.17 +1.3
3,388,866 - tldD b3244 0.18 +0.2
3,388,896 + tldD b3244 0.20 -0.5
3,388,946 + tldD b3244 0.24 +0.3
3,388,946 + tldD b3244 0.24 -0.2
3,388,946 + tldD b3244 0.24 +1.2
3,388,946 + tldD b3244 0.24 +0.3
3,388,948 + tldD b3244 0.24 +0.3
3,388,948 + tldD b3244 0.24 -0.2
3,388,954 - tldD b3244 0.24 -1.0
3,388,954 - tldD b3244 0.24 +0.3
3,388,962 - tldD b3244 0.25 +0.6
3,388,962 - tldD b3244 0.25 +0.1
3,388,998 - tldD b3244 0.27 +0.3
3,389,049 - tldD b3244 0.31 +0.2
3,389,049 - tldD b3244 0.31 -0.0
3,389,051 + tldD b3244 0.31 +0.2
3,389,090 - tldD b3244 0.34 -1.1
3,389,109 - tldD b3244 0.35 +0.5
3,389,167 + tldD b3244 0.39 +0.4
3,389,167 + tldD b3244 0.39 +1.4
3,389,172 - tldD b3244 0.39 -0.7
3,389,174 + tldD b3244 0.39 -0.3
3,389,174 + tldD b3244 0.39 -0.3
3,389,174 + tldD b3244 0.39 -0.2
3,389,182 - tldD b3244 0.40 +0.5
3,389,182 - tldD b3244 0.40 -0.4
3,389,242 + tldD b3244 0.44 +0.0
3,389,332 + tldD b3244 0.50 +1.1
3,389,332 + tldD b3244 0.50 -0.0
3,389,350 + tldD b3244 0.52 +0.3

Or see this region's nucleotide sequence