Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_15125 and PS417_15135 are separated by 281 nucleotides PS417_15135 and PS417_15140 are separated by 156 nucleotides PS417_15140 and PS417_15145 overlap by 14 nucleotides PS417_15145 and PS417_15150 are separated by 136 nucleotides
PS417_15125: PS417_15125 - glutathione S-transferase, at 3,311,105 to 3,311,698
_15125
PS417_15135: PS417_15135 - hypothetical protein, at 3,311,980 to 3,312,159
_15135
PS417_15140: PS417_15140 - 3-carboxymuconate cyclase, at 3,312,316 to 3,313,491
_15140
PS417_15145: PS417_15145 - hypothetical protein, at 3,313,478 to 3,313,768
_15145
PS417_15150: PS417_15150 - hypothetical protein, at 3,313,905 to 3,318,815
_15150
Position (kb)
3312
3313
3314 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3311.349 kb on + strand, within PS417_15125 at 3311.409 kb on - strand, within PS417_15125 at 3311.526 kb on - strand, within PS417_15125 at 3311.696 kb on + strand at 3311.784 kb on - strand at 3312.047 kb on + strand, within PS417_15135 at 3312.048 kb on - strand, within PS417_15135 at 3312.267 kb on + strand at 3312.268 kb on - strand at 3312.590 kb on + strand, within PS417_15140 at 3312.591 kb on - strand, within PS417_15140 at 3312.861 kb on - strand at 3312.909 kb on - strand, within PS417_15140 at 3313.012 kb on + strand, within PS417_15140 at 3313.077 kb on - strand, within PS417_15140 at 3313.077 kb on - strand, within PS417_15140 at 3313.172 kb on + strand, within PS417_15140 at 3313.193 kb on + strand, within PS417_15140 at 3313.193 kb on + strand, within PS417_15140 at 3313.388 kb on + strand at 3313.409 kb on + strand at 3313.600 kb on + strand, within PS417_15145 at 3313.794 kb on - strand at 3314.130 kb on + strand at 3314.184 kb on + strand at 3314.188 kb on + strand at 3314.287 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 3,311,349 + PS417_15125 0.41 -1.8 3,311,409 - PS417_15125 0.51 -1.4 3,311,526 - PS417_15125 0.71 -0.6 3,311,696 + -1.1 3,311,784 - -0.9 3,312,047 + PS417_15135 0.37 +1.0 3,312,048 - PS417_15135 0.38 -2.9 3,312,267 + -0.4 3,312,268 - +1.6 3,312,590 + PS417_15140 0.23 +0.9 3,312,591 - PS417_15140 0.23 -2.1 3,312,861 - -0.6 3,312,909 - PS417_15140 0.50 -0.3 3,313,012 + PS417_15140 0.59 -0.7 3,313,077 - PS417_15140 0.65 -1.4 3,313,077 - PS417_15140 0.65 -0.3 3,313,172 + PS417_15140 0.73 +0.4 3,313,193 + PS417_15140 0.75 -1.7 3,313,193 + PS417_15140 0.75 -1.5 3,313,388 + -1.4 3,313,409 + -0.8 3,313,600 + PS417_15145 0.42 +0.8 3,313,794 - +0.6 3,314,130 + +0.4 3,314,184 + +0.6 3,314,188 + -3.0 3,314,287 + -2.8
Or see this region's nucleotide sequence