Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12215 and PS417_12220 are separated by 98 nucleotides PS417_12220 and PS417_12225 are separated by 65 nucleotides PS417_12225 and PS417_12230 are separated by 30 nucleotides PS417_12230 and PS417_12235 are separated by 64 nucleotides
PS417_12215: PS417_12215 - lytic transglycosylase, at 2,655,400 to 2,656,815
_12215
PS417_12220: PS417_12220 - hydroxyacylglutathione hydrolase, at 2,656,914 to 2,657,681
_12220
PS417_12225: PS417_12225 - SAM-dependent methyltransferase, at 2,657,747 to 2,658,505
_12225
PS417_12230: PS417_12230 - ribonuclease H, at 2,658,536 to 2,658,988
_12230
PS417_12235: PS417_12235 - DNA polymerase III subunit epsilon, at 2,659,053 to 2,659,799
_12235
Position (kb)
2657
2658
2659 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2656.894 kb on - strand at 2657.323 kb on + strand, within PS417_12220 at 2657.360 kb on + strand, within PS417_12220 at 2657.360 kb on + strand, within PS417_12220 at 2657.362 kb on + strand, within PS417_12220 at 2657.628 kb on + strand at 2657.642 kb on - strand at 2657.642 kb on - strand at 2657.676 kb on + strand at 2657.677 kb on - strand at 2657.677 kb on - strand at 2657.718 kb on + strand at 2657.718 kb on + strand at 2659.004 kb on + strand at 2659.007 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 2,656,894 - -0.1 2,657,323 + PS417_12220 0.53 -2.3 2,657,360 + PS417_12220 0.58 -0.8 2,657,360 + PS417_12220 0.58 -0.7 2,657,362 + PS417_12220 0.58 -0.1 2,657,628 + +1.8 2,657,642 - -0.5 2,657,642 - -2.1 2,657,676 + +0.3 2,657,677 - -0.6 2,657,677 - -0.8 2,657,718 + +0.6 2,657,718 + +0.8 2,659,004 + +0.7 2,659,007 + -0.7
Or see this region's nucleotide sequence