Strain Fitness in Pseudomonas simiae WCS417 around PS417_12225

Experiment: 2'-Deoxyinosine 5 mM (C)

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntPS417_12215 and PS417_12220 are separated by 98 nucleotidesPS417_12220 and PS417_12225 are separated by 65 nucleotidesPS417_12225 and PS417_12230 are separated by 30 nucleotidesPS417_12230 and PS417_12235 are separated by 64 nucleotides PS417_12215: PS417_12215 - lytic transglycosylase, at 2,655,400 to 2,656,815 _12215 PS417_12220: PS417_12220 - hydroxyacylglutathione hydrolase, at 2,656,914 to 2,657,681 _12220 PS417_12225: PS417_12225 - SAM-dependent methyltransferase, at 2,657,747 to 2,658,505 _12225 PS417_12230: PS417_12230 - ribonuclease H, at 2,658,536 to 2,658,988 _12230 PS417_12235: PS417_12235 - DNA polymerase III subunit epsilon, at 2,659,053 to 2,659,799 _12235 Position (kb) 2657 2658 2659Strain fitness (log2 ratio) -2 -1 0 1 2at 2656.894 kb on - strandat 2657.323 kb on + strand, within PS417_12220at 2657.360 kb on + strand, within PS417_12220at 2657.360 kb on + strand, within PS417_12220at 2657.362 kb on + strand, within PS417_12220at 2657.628 kb on + strandat 2657.642 kb on - strandat 2657.642 kb on - strandat 2657.676 kb on + strandat 2657.677 kb on - strandat 2657.677 kb on - strandat 2657.718 kb on + strandat 2657.718 kb on + strandat 2659.004 kb on + strandat 2659.007 kb on + strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C)
remove
2,656,894 - -0.1
2,657,323 + PS417_12220 0.53 -2.3
2,657,360 + PS417_12220 0.58 -0.8
2,657,360 + PS417_12220 0.58 -0.7
2,657,362 + PS417_12220 0.58 -0.1
2,657,628 + +1.8
2,657,642 - -0.5
2,657,642 - -2.1
2,657,676 + +0.3
2,657,677 - -0.6
2,657,677 - -0.8
2,657,718 + +0.6
2,657,718 + +0.8
2,659,004 + +0.7
2,659,007 + -0.7

Or see this region's nucleotide sequence