Experiment: 2'-Deoxyinosine 5 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_10420 and PS417_10425 are separated by 57 nucleotides PS417_10425 and PS417_10430 are separated by 115 nucleotides PS417_10430 and PS417_10435 are separated by 69 nucleotides
PS417_10420: PS417_10420 - shikimate dehydrogenase, at 2,226,422 to 2,227,240
_10420
PS417_10425: PS417_10425 - hypothetical protein, at 2,227,298 to 2,227,945
_10425
PS417_10430: PS417_10430 - hypothetical protein, at 2,228,061 to 2,228,570
_10430
PS417_10435: PS417_10435 - serine/threonine protein kinase, at 2,228,640 to 2,229,860
_10435
Position (kb)
2228
2229 Strain fitness (log2 ratio)
-2
-1
0
1 at 2227.178 kb on + strand at 2227.178 kb on + strand at 2227.179 kb on - strand at 2227.458 kb on + strand, within PS417_10425 at 2227.840 kb on - strand, within PS417_10425 at 2227.957 kb on + strand at 2227.958 kb on - strand at 2227.979 kb on - strand at 2228.066 kb on + strand at 2228.296 kb on - strand, within PS417_10430 at 2228.304 kb on - strand, within PS417_10430 at 2228.554 kb on - strand at 2228.554 kb on - strand at 2228.554 kb on - strand at 2228.569 kb on - strand at 2228.689 kb on - strand at 2228.761 kb on - strand at 2229.004 kb on + strand, within PS417_10435 at 2229.568 kb on - strand, within PS417_10435
Per-strain Table
Position Strand Gene LocusTag Fraction 2'-Deoxyinosine 5 mM (C) remove 2,227,178 + +0.2 2,227,178 + -0.1 2,227,179 - +1.3 2,227,458 + PS417_10425 0.25 -0.0 2,227,840 - PS417_10425 0.84 +1.1 2,227,957 + -0.0 2,227,958 - +0.6 2,227,979 - +0.6 2,228,066 + -0.3 2,228,296 - PS417_10430 0.46 -0.1 2,228,304 - PS417_10430 0.48 -1.9 2,228,554 - -0.1 2,228,554 - +0.7 2,228,554 - -0.5 2,228,569 - +0.0 2,228,689 - -2.7 2,228,761 - -0.3 2,229,004 + PS417_10435 0.30 -1.0 2,229,568 - PS417_10435 0.76 -0.5
Or see this region's nucleotide sequence